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We narrowed to 652 results for: SIM

Showing: 161 - 180 of 652 results

  1. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...techniques and strategies one can employ beyond simply incubating cells with single-fluorophore-conjugated... B cells, and dendritic cells. In this case, we simply need to label the lineage markers of all cell types...selected color will not be CD8+ T cells and we can simply “dump” them by gating on the negative population... new methods of target visualization beyond the simple surface labeling. Let’s briefly walk through the...

  2. Evolution of Lab Techniques

    Type
    Blog Post
    ...next-generation sequencing techniques to answer similar experimental questions (see below). Importantly...location of a given nucleotide, a researcher (or a simple computer program) can then reproduce the DNA sequence...generation DNA sequencing machines (9). Today, similar technologies, sometimes referred to as sequence...Kathleen, and Daniel Nathans. "Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus...

  3. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...reason most people enter postdoctoral training. Similarly, there are several factors to consider when selecting... week planning an experiment, even a relatively simple experiment. However, by taking this time, you can...result? Is there a tractable question or are you simply describing a phenomenon? Exploration can be important...Plus, presentations benefit from practice. If you simply recycle slides from the last time you gave a presentation...

  4. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...cassette (Dickinson et al., 2015) made this step as simple as heat-shocking to induce the expression of a ...my initial direct screening efforts, which were similar to the approach detailed by Paix et al. Although...Addgene. In 2019, Farboud et al. described another similar co-conversion marker (repair of a zen-4(cle10ts...choosing. Tools such as SapTrap Builder greatly simplifies design of these constructs, allowing novice researchers...

  5. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...using the csRNP method is as simple as doing routine PCR assays. Here is a simple to-do-list to follow when...spectrometry to identify the interacting proteins. Similarly, in ChIP-seq assays, chromatin fragments that ...successfully knock-in non-expressed genes with similar efficiency, Dewari et al. 2018). Using the first...

  6. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...Fluorescent proteins (FPs) offer scientists a simple yet powerful way to tag cellular proteins and investigate...fluorescence when illuminated with blue light (1). Similar to PA-GFP, scientists later developed red fluorescent...photoactivatable fluorescent protein for your experiment Similar to performing experiments with standard FPs, before...live imaging of proteins, organelles, and cells. Similar to FPs, PA-FPs allow for non-invasive labelling...

  7. AAV Packaged on Request is Here!

    Type
    Blog Post
    .... To start the AAV packaged on request process, simply navigate to your plasmid of interest. If it’s eligible...cargo you requested at the quality you expect. Similar to our on-demand viral service, we expect titers...There are no quotes or special processes involved; simply request your plasmid of interest in your desired...

  8. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ... used as the first letter of a plasmid name and simply denotes that the object is a ‘plasmid’. “p” is ... empty backbone name in your plasmid name. This simple piece of information can often convey many important...pBACKBONE-Flag-hGene-EGFP or pBACKBONE-Flag-hGene(wt)-EGFP These simple rules allow any scientist to know what is in a ...

  9. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...edited strand. The first iteration of prime editor simply fused the wild-type Moloney Murine Leukemia Virus...or exceed PEmax editing efficiencies for short, simple edits. PE6c and PE6d further evolved the Tf1 and...pegRNAs, but combining PE7 with epegRNAs resulted in similar or reduced editing efficiencies, likely because...Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt...

  10. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ... the Cellosaurus STR similarity search tool (CLASTR) to find cells lines similar to your STR profiles ...CLASTR results by minimal number of markers and similarity score match. This tool’s algorithm can be adjusted...

  11. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...control over neuronal activity. While they serve similar purposes, both techniques offer researchers different...resolution as receptors can be turned on and off simply with the presence or absence of the correct wavelength...flexible as it relies on designer ligands that can be simply introduced by injection. The main challenge in ...

  12. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate sensors, the new dLight1...s lab has created the GRABDA sensor based on a similar strategy: a GFP variant bound to a DA receptor,...cyclase in caudate nucleus of rat brain, and its similarity to the “dopamine receptor”." Proceedings of the...

  14. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...Interspaced Short Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too...into a single strand that folds back on itself, simplifying the CRISPR-Cas9 system for laboratory applications...post on the structural biology of Cas9. Want a simpler guide to keep at your desk? We’ve got a CRISPR ...

  15. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...and cell lines. Although ChIP-seq is a relatively simple and robust protocol it does have limitations. The...change offers a significant improvement and simplification of the original technique, specifically in ...CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids for isolating...

  16. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...provides a combinatorial indexing strategy that simultaneously tracks clonal history and cell identity at ...that cells with common ancestors tended to have similar reprogramming outcomes. For example, cells with...with shared CellTagD13 tags often had similar gene expression pattern changes and reprogramming outcomes...

  18. RUBY-Red Siliques

    Type
    Blog Post
    ...equipment. Reporters for studying gene expression have similar drawbacks, often requiring the destruction of plants... events and am currently planning to use it to simplify my work with an Arabidopsis mutant that can’t ...unfamiliar with these mutant plants. Using RUBY to simplify the identification of these mutants will speed...

  19. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...multifunctional fibers, embedded, and validated simultaneously in the brain and the intestines of mice. The...organisms, including the Anikeeva lab’s work on a similar neural fiber (Park et al., 2019). This provided... To operate both types of implanted fibers simultaneously, a mini-Bluetooth system named NeuroStack was...

  20. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...Viruses are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can... even decide if they’re alive or not. Yet these simple, small particles have quite the outsized impact...
Showing: 161 - 180 of 652 results