We narrowed to 232 results for: SPL;

1. Antibodies 101: ChIP

   Type

   Blog Post

   ...choose, you’ll need to reserve a small aliquot, split into two parts to (1) run a DNA gel to confirm fragment...

2. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   ...David Liu Lab similarly described xCas9, which displayed the ability to target multiple PAM sequences (...

3. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   ...is also common. Overlap extension PCR is used to splice together two PCR amplicons based on sequence homology...

4. CRISPR Plasmids - Empty gRNA Vectors

   Type

   Collection

   ...pLSC-5 62889 Mammalian/Lentiviral BsmBI yes, cut (split) S. pyogenes Zhang pICSL01009::AtU6p 46968 Plant...

5. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

   Type

   Blog Post

   ...the Uppalapati and Woolley lab developed a phage display technique using small scaffold proteins to identify...

6. Choosing the B(right)est Fluorescent Protein: Photostability

   Type

   Blog Post

   ...top of photobleaching, fluorescent proteins may display reversible intensity changes (Shaner et al, 2008...

7. Transferable Skills Guide: Public Speaking

   Type

   Blog Post

   ...the nerve to do what I did. Until that panicked, split-second decision, I had always thought I had an obligation...

8. The time and cost required to make a plasmid

   Type

   Blog Post

   ...same time period. For ease of calculations*, let’s split the difference and put scientific inflation at… ...

9. CRISPR 101: Cas9 vs. The Other Cas(s)

   Type

   Blog Post

   ...cleavage and 2). cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less...

10. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type

    Blog Post

    ...SpCas9 N692A/M694A/Q695A/H698A). This new variant displays comparable on-target activity and similar or decreased...

11. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    ...reduced. Third generation packaging systems further split the viral genome by removing the viral Rev gene ...

12. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    ...purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral genes and transgene...

13. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    ...no detectable editing. Additionally, off-target spleen editing was not detected and Nanoblade injection...

14. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    ...chicken beta actin promoter, and rabbit beta-globin splice acceptor, contains sequence that is over 80% GC...

15. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    ...or face shields are required whenever there’s a splash or spray risk in BSL-1. In BSL-2 labs, eye protection...

16. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    ... reliability. Notably, the SpRY variant didn’t display higher off-target effects than SpCas9, a pleasant...

17. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    ...direct evolution, capsid shuffling, and peptide displays. These capsids will be selected both for their...

18. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    ... cleavage. Now, two new studies show that Cpf1 displays lower off-target editing than Cas9, confirming...

19. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    ...happens often because the HDR inserted sequence splits up the guide sequence.) If neither of the above...

20. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    ...efficiency is not known, this is easily achieved by splitting transfected cells into 3 plates at different densities...