Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 466 results for: Sele

Showing: 161 - 180 of 466 results

  1. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...shuffling, and peptide displays. These capsids will be selected both for their ability to evade the immune system...specificity through control of gene expression and the selection of specific promoters may also help AAV vectors...

  2. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... Figure 3: ASAP5 for voltage imaging. A) ASAP5 selected for its improved kinetics and responsivity in ...context-dependent responses and molecular patterns of selectivity. With 10 plasmids available as a kit, plus 21...

  3. Pooled Library Amplifications

    Type
    Blog Post
    ...Re-transformation of the SPLINTR Libraries Protocol.   Selecting your strain for amplification Since pooled libraries...your transformation, any of these options work! Selecting your media Lastly, you need to decide between ...

  4. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...affinity for a target – and this serotype can be selected to preferentially target your cell type of interest...applications, the ability to direct your voltage sensor selectively is especially essential. If you want to learn...

  5. PhD Applications After COVID

    Type
    Blog Post
    ...students to do rotations in different labs before selecting an advisor, it is always a good idea to introduce...receiving applications, universities typically invite a select group of students to continue the application process...

  6. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...programs are optimized differently and you may want to select one (or both) depending on the information you ... click the BLAST button, consider the Program Selection parameter, as this will affect the amount of time...

  8. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...Find plasmids from this paper at Addgene. East-Seletsky, Alexandra, et al. "Two distinct RNase activities...Find plasmids from this paper at Addgene. East-Seletsky, Alexandra, et al. "RNA Targeting by Functionally...

  11. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...actuators and sensors at Addgene. Cell marking/selection: Expression constructs like plasmids often include...This can serve as an alternative to antibiotic selection. Plasmids of this type may have the GFP under ...

  12. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    .... The research described here is only a small selection of the increasing number of studies using viral...Amine, Vijay Chandrasekar, and Jean-Luc Dreyer. "Selective lentiviral-mediated suppression of microRNA124a...

  14. Bioinformatics at Addgene

    Type
    Blog Post
    ...several platforms for engineering pipelines, we selected Nextflow, a workflow management system for scientific...for rapid testing and refinements, we specified a select small number of input file names or filtered the...

  15. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... plasmids from this paper at Addgene. 2. East-Seletsky, Alexandra, et al. "Two distinct RNase activities... plasmids from this paper at Addgene. 4. East-Seletsky, Alexandra, et al. "RNA Targeting by Functionally...

  16. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...reasons. Exposure to antibacterial drugs provides a selection pressure, killing off bacteria that are susceptible...stop a treatment early, you may be un-wittingly selecting for less susceptible bacteria that will be more...

  17. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...variables within an experiment, both the design and selection of the positive control plasmids should be highly... Expression Level of Gene X   The design and selection of proper experimental controls is not a trivial...

  18. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...chloramphenicol resistance cassette for positive selection and a p15A origin of replication, making it incompatible...Your strain may require additional antibiotic selection or a special growth media, or if your plasmid ...

  19. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...Thermo Fisher Scientific's Biotinylation Reagent Selection Tool. AviTag and BirA Proteins can also be biotinylated...Photobiotinylation Photobiotin can be used to non-selectively label DNA and RNA. Photobiotin acetate consists...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...intended edit is the biggest determinant when selecting a type of donor. ssDNA templates (referred to ...large insertions such as fluorescent proteins or selection cassettes. This class of template should have ...
Showing: 161 - 180 of 466 results