We narrowed to 249 results for: TIR

1. The Scientific Conference Poster Session: Tips for Success

   Type

   Blog Post

   ...gone up to a poster where the presenter spends the entire time talking to the poster, as opposed to engaging...

2. Transferable Skills: Negotiation

   Type

   Blog Post

   ...resolution. Creative negotiation can also decrease or entirely eliminate the feeling of conflict many of us associate...

3. Polymerase Chain Reaction Overview and Applications

   Type

   Blog Post

   ...exponentially replicate the target DNA of interest. This entire reaction used to be done manually in water baths...

4. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   ...the antibodies were able to immunoprecipitate the entire complex in three different cell lines. The Gräslund...

5. The AAVantages of AAV in CRISPR Screens

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   Blog Post

   ...still beats lentivirus. In mice, AAV is almost entirely non-immunogenic — meaning when conducting in vivo...

6. Fluorescent Proteins 101: Fluorescent Protein Timers

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   Blog Post

   .... A special class of FPs, the FP timers, add an entire new dimension to monitoring localization; using...

7. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   ...PEG solution. PEG takes several hours on a heated stir plate to dissolve and requires monitoring so that...

8. Plasmids 101: Optimizing Plasmid Yields

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   Blog Post

   ... a plasmid whose copy number is unknown, it is entirely possible that you are working with a low copy ...

9. Anatomy of a Plasmid Page at Addgene

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   Blog Post

   ...in the depositing lab, although you may have an entirely different use in mind! This section of our plasmid...

10. Light Sheet Fluorescence Microscopy

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    Blog Post

    ...  Furthermore, point-by-point scanning along the entire specimen makes imaging too slow and unwieldy for...

11. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type

    Blog Post

    ..., the drive could eventually spread through the entire wild population with unpredictable ecological effects...

12. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    ...the same mRNA. The term "self-cleaving" is not entirely accurate, as these peptides are thought to function...

13. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    ...tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses and wheat, but we do work...

14. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    Blog Post

    ...about CRISPR alone, and Zimmer himself has written entire books about optogenetics. Defining science and ...

15. Summer SciComm Series: Modes of Communication

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    Blog Post

    ...distribution.  Static. Can be difficult to convey an entire story or context via still images. Some things ...

16. Great Results Start with Great Standard Curves

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    Blog Post

    ...software, don’t give up! Rather than plotting the entire data set, plot only the linear portion. This allows...

17. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post

    ...low rate, there is evidence that zeocin is never entirely detoxified, even when Sh ble is expressed (Trastoy...

18. Getting to Know Expansion Microscopy

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    Blog Post

    ...optimization and careful execution, but they cannot be entirely eliminated. It is recommended that you image samples...

19. Uncovering Ancestral Bias in CRISPR Research

    Type

    Blog Post

    ...racial bias from work being done today, is a nearly entirely separate effort from acknowledging its existence...

20. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    ... Chen co-founded Addgene in 2004. They worked tirelessly to get Addgene from a concept to an up and running...