Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 167 results for: Tub;

Showing: 161 - 167 of 167 results

  1. Western Blot

    Type
    Protocol
    ... buffer e.g., RIPA lysis buffer Microcentrifuge tubes BCA Assay, Thermo Fisher 23227 β-mercaptoethanol...1 mL of 1X PBS and transfer to a microcentrifuge tube. Centrifuge for 5 min at 100 x g . Carefully remove... Transfer supernatant to a clean microcentrifuge tube. Use the lysate immediately or store at -80 °C until...

  2. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...When ready to grow your culture, add liquid LB to a tube or flask and add the appropriate antibiotic to the... you will usually want to start 2 mL in a falcon tube, but for larger preps you might want to use as much...

  3. Fluorescence Titering Assay

    Type
    Protocol
    ...Microcentrifuge tubes, Neptune 3745.X 6-well tissue culture treated dish, Corning 3516 15 mL conical tubes, VWR ...

  4. Colony Formation Titering Assay

    Type
    Protocol
    ...Microcentrifuge tubes, Neptune 3745.X 6-well tissue culture treated dish, Corning 3516 15 mL conical tubes, VWR ...

  5. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...DNase buffer Nuclease-free water Microcentrifuge tubes 96-well optical plate Pipette tips General Considerations...for 2 min to bring the sample to the bottom of the tube. Run the following protocol in your qPCR instrument...

  6. Pouring LB Agar Plates

    Type
    Protocol
    ...potential contaminants, certain types of spores will stubbornly survive even under the harsh conditions in the... leave room for your bottle of molten gel mix, a tube rack containing the appropriate antibiotics, and...

  7. Gibson Assembly Protocol

    Type
    Protocol
    ... scar between joined fragments. Fewer steps. One tube reaction. Can combine many DNA fragments at once...
Showing: 161 - 167 of 167 results