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We narrowed to 204 results for: aav vector plasmid

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Showing: 161 - 180 of 204 results

  1. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...cloned into a vector containing Cas9 and the PITCh-gRNA. Step 3: Contransfect the PITCh vector with the vector...Guide Pages Find CRISPR Plasmids for Your Experiments Deposit Your New Plasmid Tools with Us  ...repair, making it easier to construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed...Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell lines can be created in about... knock-ins, some labs have used NHEJ for whole plasmid integration; however, since NHEJ is error-prone...just upstream of a stop codon. Briefly, the PITCh vector should be constructed with 5’ and 3’ microhomology...cloning. An overview of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and ...

  2. Biosensor AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor AAV Browse our...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...filtering options. *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 5, 9, rg* GENIE Project 162379 pGP-AAV-syn-FLEX-jGCaMP8f-WPRE...169258 AAV-hSyn-Soma-jGCaMP8f Syn soma-jGCaMP8f none Constitutive 1, 9, rg* Marianne Fyhn 176750 AAV-CamKIIa-jGCaMP8f-WPRE...176753 AAV-mDlx-jGCaMP8f-WPRE Dlx jGCaMP8f none Constitutive 1, 9, rg* Loren Looger 176756 AAV-EF1a-jGCaMP8f-WPRE...pGP-AAV-syn-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 5, 9, rg* GENIE Project 162377 pGP-AAV-syn-FLEX-jGCaMP8s-WPRE...

  4. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ... we would recommend using a cDNA overexpression vector rather than going through all the troubleshooting...et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences from a given... CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on Addgene.org Find Cas9 Activators Find...

  5. Plan Your Experiment

    Type
    Collection
    ... lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. Both lentiviral...'s collection of CRISPR plasmids . Delivery Using Viral Vectors Viral vectors are a great option, as they...adeno-associated virus (AAV) vectors are the most common. Using viral vectors takes a bit more prep work...about viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral ...lentiviral and AAV vectors are safe to use in the lab, but AAVs exhibits the lowest immunogenicity and are...frequently use AAVs to develop CRISPR-based therapeutics. One limitation of AAV vectors is that the packaging...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...

  6. Optogenetics AAV Preps

    Type
    Collection
    ...Viral Vector Packaging Service AAV Optogenetics Viral Vector Packaging Service: Optogenetics AAV Optogenetic...Optogenetics AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV collection, ...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...filters below to find ready-to-use AAV preparations of optogenetics plasmids available in Addgene's collection...20071 pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Karel Svoboda 58892 pAAV-GFAP104-ChR2-mCherry...28017 AAV-CAG-hChR2-H134R-tdTomato CAG ChR2/H134R tdTomato Constitutive rg* Karel Svoboda 75470 pAAV-CAG-FLEXFRT-ChR2...

  7. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... Viral Vector Packaging Service Packaged on Request Scope of Service AAV Packaged on Request: Scope of...least 4 x 10 12 GC/mL for the AAV1, AAV5, AAV6, AAV8, AAV9, AAV11, or AAV Retrograde serotypes and 2 x ... particular AAV prep, mostly likely due to one of the following reasons: Transfer plasmids with sequence...any viral vector. Addgene performs QC measures which verify the identity of the viral vector, but not ...and limited use label licenses (LULLs). Titer For AAV preps produced through our Packaged on Request service...the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids with a cargo that may reduce titer due to toxicity...guarantees above. We will have our expert viral vector production team carefully review your cargo/serotype...

  8. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase...expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT vector ensures...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with the...

  9. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout...Site-Specific Recombinases Cre Plasmids Flp Plasmids Dre Plasmids Other Recombinase Plasmids Recombinase-Dependent...experiments using AAV vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing...”, or “light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene... Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources ...View Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene...Site-Specific Recombinase Plasmids ID Plasmid Description Gene/Insert Vector Type Promoter PI Return to...

  10. Tetracycline Inducible Expression

    Type
    Collection
    ...pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA efficacy by fluorescence, use Plasmid #35626 as a ...targeting Alice Ting 85040 pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression of...Promoters Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research Plasmids References Das, A. T., Tenenbaum...promoter Tet-On 3G rtTA David Vereide 120309 pAAV-FAH-rtTA3G AAV vector to express Tet-On 3G transactivator under...-On vector expressing TetR from CMV promoter TetR Eric Campeau , Paul Kaufman 175274 pAAV-rtTA AAV Tet-On.... tTA Edward Hsiao 99118 pAAV-CAG-tTA AAV Tet-Off vector, expresses tTA from the CAG promoter. See article...Tet-Off tTA-Advanced Takeshi Imai 104109 pAAV-Syn1-tTA AAV Tet-Off vector, expressed tTA from the hSyn promoter...

  11. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription... search for popular plasmids expressing luciferase. We also offer ready-to-use AAV preparations of select...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV-CRISPR knockout plasmid. Renilla luciferase is used to monitor...pscAAV-CAG-RLuc Renilla CAG Vector for packaging self-complementary AAV expressing Renilla luciferase Mark Kay 140328...

  12. Chemogenetics Plasmids

    Type
    Collection
    ...ready-to-use AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type ...Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection Chemogenetics plasmids encode genetically engineered...Chemogenetics Plasmids Browse, sort, or search this table to find chemogenetics plasmids in our collection...Resources Chemogenetics Guide Viral Vector Packaging Service In-Stock AAV: Chemogenetics Fluorescent Biosensors...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids for chemogenetic experiments, including popular DREADDs and PSAMs. Plasmid... Plasmid Collections ...

  13. Optogenetics Plasmid Collection

    Type
    Collection
    ...ready-to-use AAV preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter... Plasmid Collections Optogenetics Plasmids Optogenetics Plasmid Collection Microbial...Microbial Opsin Plasmids Optical Switch Plasmids Additional Resources Optogenetics plasmids encode proteins ...Microbial Opsin Plasmids Browse, sort, or search the table below for the optogenetics plasmids for neuronal... top Optical Switch Plasmids Browse, sort, or search the table below for plasmids that use light to control...transcription", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter PI Return to top ...Resources Optogenetics Guide Viral Vector Packaging Service In-Stock AAV: Optogenetics Fluorescent Biosensors...

  14. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones...pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab ...gene promoters C. elegans vector kit - Collection of plasmids from the Fire Lab L4440 -...expression vector with N-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF ...

  15. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Science AAV Enhancer Collection Allen Institute for Brain Science AAV Enhancer Collection Plasmids Publications...Publications Resources Enhancer AAV plasmids are plasmids which use genomic regulatory sequences — termed...Collection Caltech Systemic Capsids AAV Resources AAV Packaged on Request AAV Guide...Find AAV enhancer plasmids for fluorescent labeling and recombination across brain regions and populations...populations using systemic AAV delivery. The collection includes the top vectors with the highest specificity...Enhancer Plasmids The Allen Institute for Brain Science Enhancer Collection contains best-in-class AAV plasmids...Addgene ID Plasmid Allen Institute ID Enhancer ID FP/Recombinase Labeled Population Region 230551 pAAV-AiE2638m-minBG-SYFP2...

  16. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression... Ligand Plasmids HaloTag Chloralkane (CA) pMH-Halo tag - Mammalian Gateway Destination Vector pENTR4-HaloTag...Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf - Xenopus Expression pSNAP-tag (T7) Vector - Bacterial...pCLIP-tag (m) Vector - Mammalian Expression pENTR4-CLIPf (w877-2) - Gateway Entry Vector pET51b-His-TEV-CLIP-tag...Fluorescent Protein Collection Luciferase Plasmid Collection Promega Plasmid Collection Botman-Teusink Yeast Fluorescent...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...mGreenLantern - Mammalian Expression pAAV-CAG-mGreenLantern - Mammalian AAV Expression pBAD-mGreenLantern -...

  17. Let There Be LITE Plasmids

    Type
    Blog Post
    ... (blue) light switch. Zhang’s team devised the plasmids now available in Addgene’s repository for use ...Zhang showed they could package LITEs in viral vectors and genetically target them to specific cell populations...biological processes and disease states. While LITE plasmids have clear application to anyone with an interest...offers a tip: save yourself some time by making an AAV1 supernatant. (The details can be found here.) So...

  18. Depositor Collections

    Type
    Collection
    ...T7 RNAP Plasmids Repressor Plasmids Sigma (σ) Factor Plasmids Terminator Plasmids Viral Vectors pLEG/pREG...pREG Modular Viral Vector System - Dankort Gene Vector Core Viral Vectors - Oka...synthetic biology, and viral vector plasmids. Plasmid... Plasmid Collections Depositor Plasmid Collections Depositor Collections...Addgene's repository contains 173,199 plasmids, which includes plasmid collections from depositors. To help... you find it helpful! You May Also Like: Plasmid Kits Plasmid Special Collections Pooled Libraries Antibodies...Recombinant mAb Plasmid Collection Disease Research Michael J. Fox Foundation Plasmid Resource iPSC Neurodegenerative...

  19. Neurodegeneration Research Collection

    Type
    Collection
    ...Localization , Optogenetics Plasmids , and Chemogenetics Plasmids . Find plasmids for endogenous gene tagging... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant... platform . (Tian et al. Neuron. 2019) AAV Viral Preps Find AAV viral preps for systemic delivery of viral...Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids provided by the foundation...Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and...

  20. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ... Bioengineering Find the optogenetic repressor plasmids Cytosine base editors with minimized off-target...paper in Nature Biotechnology Find the base editor plasmids CRISPRi in Caulobacter crescentus Figure...once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system...lab developed new fluorescently labeled piggyBac vectors to deliver uniform and sustained expression of ... Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation ...
Showing: 161 - 180 of 204 results