Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 198 results for: aav vector plasmid

Showing: 161 - 180 of 198 results

  1. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... Viral Vector Packaging Service Packaged on Request Scope of Service AAV Packaged on Request: Scope of... the AAV1, AAV5, AAV6, AAV8, AAV11, or AAV Retrograde serotypes and 2 x 10 12 GC/mL for the AAV2 serotype... particular AAV prep, mostly likely due to one of the following reasons: Transfer plasmids with sequence...any viral vector. Addgene performs QC measures which verify the identity of the viral vector, but not ...and limited use label licenses (LULLs). Titer For AAV preps produced through our Packaged on Request service...the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids with a cargo that may reduce titer due to toxicity...guarantees above. We will have our expert viral vector production team carefully review your cargo/serotype...

  2. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout...Site-Specific Recombinases Cre Plasmids Dre Plasmids Flp Plasmids Other Recombinase Plasmids Recombinase-Dependent...experiments using AAV vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing...”, or “light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene... Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources ...View Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene...Site-Specific Recombinase Plasmids ID Plasmid Description Gene/Insert Vector Type Promoter PI Return to...

  3. Tetracycline Inducible Expression

    Type
    Collection
    ...Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids Collection Tetracycline...pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA efficacy by fluorescence, use Plasmid #35626 as a ...targeting Alice Ting 85040 pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression of...promoter Tet-On 3G rtTA David Vereide 120309 pAAV-FAH-rtTA3G AAV vector to express Tet-On 3G transactivator under...-On vector expressing TetR from CMV promoter TetR Eric Campeau , Paul Kaufman 175274 pAAV-rtTA AAV Tet-On.... tTA Edward Hsiao 99118 pAAV-CAG-tTA AAV Tet-Off vector, expresses tTA from the CAG promoter. See article...Tet-Off tTA-Advanced Takeshi Imai 104109 pAAV-Syn1-tTA AAV Tet-Off vector, expressed tTA from the hSyn promoter...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription... search for popular plasmids expressing luciferase. We also offer ready-to-use AAV preparations of select...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV-CRISPR knockout plasmid. Renilla luciferase is used to monitor...pscAAV-CAG-RLuc Renilla CAG Vector for packaging self-complementary AAV expressing Renilla luciferase Mark Kay 140328...

  5. Chemogenetics Plasmids

    Type
    Collection
    ...ready-to-use AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type ... Plasmid Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection You may...Guide Viral Service: Chemogenetics AAV Blog: Chemogenetics Viral Vectors 101 Blog: Chemogenetics vs. Optogenetics...Chemogenetics Plasmids Browse, sort, or search this table to find chemogenetics plasmids in our collection...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Optogenetics Chemogenetics plasmids encode genetically engineered receptors that are activated by specific...collection. For example, search for "hM4D mCherry", "KORD AAV", "LMO", "Sternson", or other terms. Looking for ...

  6. Let There Be LITE Plasmids

    Type
    Blog Post
    ... (blue) light switch. Zhang’s team devised the plasmids now available in Addgene’s repository for use ...Zhang showed they could package LITEs in viral vectors and genetically target them to specific cell populations...biological processes and disease states. While LITE plasmids have clear application to anyone with an interest...offers a tip: save yourself some time by making an AAV1 supernatant. (The details can be found here.) So...

  7. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs into one vector, and plasmids...Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...time. Single plasmids containing both the gRNA and a Cas protein act as an all-in-one vector, but their ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited...Lentiviral, AAV, Bacteria, Drosophila, Yeast, Plant, Xenopus, Worm, or Other. Whether the plasmid contains...

  8. Optogenetics Plasmid Collection

    Type
    Collection
    ...ready-to-use AAV preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter... Plasmid Collections Optogenetics Plasmids Optogenetics Plasmid Collection You may ...Guide Microbial Opsin Plasmids Optical Switch Plasmids Background Optogenetics plasmids encode proteins that...like... Optogenetics Guide AAV Viral Service Biosensors Chemogenetics Plasmids Chemogenetics Guide Fluorescent...Microbial Opsin Plasmids Browse, sort, or search the table below for the optogenetics plasmids for neuronal... top Optical Switch Plasmids Browse, sort, or search the table below for plasmids that use light to control...transcription", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for Viral...

  9. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Capsids AAV Packaged on Request AAV Guide Plasmids Publications Enhancer AAV plasmids are plasmids which...Find AAV enhancer plasmids for fluorescent labeling and recombination across brain regions and populations...Institute for Brain Science AAV Enhancer Collection Allen Institute for Brain Science AAV Enhancer Collection ...populations using systemic AAV delivery. The collection includes the top vectors with the highest specificity...Enhancer Plasmids The Allen Institute for Brain Science Enhancer Collection contains best-in-class AAV plasmids...Addgene ID Plasmid Allen Institute ID Enhancer ID FP/Recombinase Labeled Population Region 230551 pAAV-AiE2638m-minBG-SYFP2...window) . Figure 1: Selection, prep, and testing of AAV enhancers in mice. Enhancers were selected from the...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones...pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab ...gene promoters C. elegans vector kit - Collection of plasmids from the Fire Lab L4440 -...expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF...

  11. Neurodegeneration Research Collection

    Type
    Collection
    ... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant... Nat Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV viral preps for systemic delivery of viral...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids provided by the foundation...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other...

  12. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ... Bioengineering Find the optogenetic repressor plasmids Cytosine base editors with minimized off-target...paper in Nature Biotechnology Find the base editor plasmids CRISPRi in Caulobacter crescentus Figure...once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system...lab developed new fluorescently labeled piggyBac vectors to deliver uniform and sustained expression of ... Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation ...

  13. Hot Plasmids - October 2022

    Type
    Blog Post
    ...-driven viral vectors (AAV1) - and you can also find several other constructs in plasmid form. ...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ...Think we missed something hot? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...JEDI-2p viral preps here (and here!)Find JEDI-2p plasmids here!  Liu, Zhuohe et al. Sustained deep-tissue... improve the performance of HcKCRs. Find HcKCR plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins...’ in the Addgene catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a...myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc...

  14. Depositor Collections

    Type
    Collection
    ...T7 RNAP Plasmids Repressor Plasmids Sigma (σ) Factor Plasmids Terminator Plasmids Viral Vectors pLEG/pREG...synthetic biology, and viral vector plasmids. Plasmid... Plasmid Collections Depositor Plasmid Collections Depositor Collections...Addgene's repository contains 161,545 plasmids, which includes plasmid collections from depositors. To help... you find it helpful! You May Also Like: Plasmid Kits Plasmid Special Collections Pooled Libraries Antibodies...Recombinant mAb Plasmid Collection Disease Research Michael J. Fox Foundation Plasmid Resource iPSC Neurodegenerative...Collection Rett Syndrome Plasmid Collection and Resource Center TREAT-AD Plasmid Collection Visualization...

  15. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Adeno-Associated Viral (AAV) Vector Guide Jump To: AAV Vectors Vector Production Integration AAV Types Common Uses...the packaging plasmid. For a summary of all AAV plasmid components, see the AAV Plasmid Elements table...Parts of the AAV Transfer Plasmid Parts of the AAV Packaging Plasmid Producing Your rAAV AAV Serotypes and...different plasmid. In order to produce rAAV vectors, three plasmids are needed: Transfer plasmid (also known...Figure 2: Plasmids required for rAAV vector production. Created with BioRender.com. AAV Vector Production...both key advantages of rAAV vectors. AAV Types Serotypes AAV serotypes are AAV variants that differ in... virus (AAV) integration sites in AAV virions during AAV wild-type and recombinant AAV vector production...

  16. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...below for CRISPR plasmids for RNA editing in mammalian systems. Mammalian ID Plasmid Gene/Insert PI Publication...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...Plasmids: RNA Editing Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...capabilities but are small enough to be packaged in AAV particles. Want more information on the wide variety...

  17. Mammalian RNAi Tools

    Type
    Collection
    ...delivery of a plasmid. shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred...BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a ... Plasmid Collections Mammalian RNAi Mammalian shRNA Tools for RNAi Browse Plasmids Resources ... backbone, with some retroviral and AAV backbone options. Plasmids for constitutive expression as well...argonaute, please search our full site . ID Plasmid Vector Type PI Publication Additional Resources Addgene...pLKO.1 cloning vector protocol Other Addgene viral protocols Lentiviral packaging plasmids Web Resources...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...

  18. Bioinformatics at Addgene

    Type
    Blog Post
    ... NGS Plasmid Validation: SeqwellHow to Keep a Lab Notebook for Bioinformatics AnalysesAAV Vector Quality...days, we would ‘spot-check’ critical regions of plasmids deposited with us via Sanger sequencing, while...while more recently we have moved to full plasmid sequencing at our facility using Illumina MiSeq Next Generation...lab work as we grew. As the number of deposited plasmids increased, the accompanying sequencing data collection...transform the raw sequencing data into a complete plasmid sequence that would be ready for our QC team to...With our outline of individual steps to automate plasmid sequence assembly, and possible solutions for each...up with our sequencing instrument (hundreds of plasmids each week!), we already planned to use cloud computing...

  19. Plasmid Collections

    Type
    Collection
    ...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 161,545 plasmids . We've...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Consortium Krüppel-like factors (KLF) Plasmid Resource RESOLUTE Plasmid Collection for Solute Carrier Proteins... Find deposited plasmids for specific disease research areas. Cancer Research Plasmids and Resource Michael... Syndrome Plasmid Collection and Resource Center Do you have suggestions for other plasmids that should...

  20. General Transfection

    Type
    Protocol
    ...therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ...protocol to transfect mammalian cells with your plasmid of interest. Protocols...General Transfection You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral ..., the plasmid should also be propagated in an endonuclease negative E. coli strain such as NEB stable....is prepared, transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1–2 ...of HEK293T cells optimized for viral production (AAVpro or Lenti-X), but any HEK293T cell line should work...
Showing: 161 - 180 of 198 results