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We narrowed to 397 results for: abo.4

Showing: 161 - 180 of 397 results

  1. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...the contact to start a direct collaboration between researcher and expert; 4) Credit all contributions. ...our blog post about remote collaborations Read our Transferable Skills Guide article about external collaborations...needed. In the example above, for example, we fostered a formal scientific collaboration between two research...project. We think that these collaborations add enormous value. A discussion about a protocol can save weeks..., the researchers did not have access to a P3 laboratory (BSL-3), a high-security facility needed to perform...approach was useful in humans. Then, one of them heard about our online platform, called Crowdfight, and filed...just the right infrastructure and offered to collaborate with them. The partnership was fruitful and a...

  2. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ... importantly, it’ll help you define salary costs. 4. Ascribe a cost structure associated to these tools...others will help prove that you will be an asset. 4. Always ask, “Is there an alternative?” As scientists...a successful negotiation and collaboration. We’d love to hear more about your experiences and opinions...can get away with using computer modeling and collaborations rather than using animals, then that’s a cost...associated with old equipment, like service repairs, labour costs, and loss of time, can exceed the cost of...Everything is negotiable. Try asking for discounts, collaborations, access to internal award applications, etc...This is a non-profit organization. Therefore, collaboration and community is the ultimate value proposition...

  3. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...efficacy of your antibody. When compared, 4% PFA and 0.1% saponin; 4% PFA and 0.1% Triton X-100; and methanol...and KO cells were mixed in a 1:1 ratio and fixed in 4% PFA and permeabilized in 0.1% saponin. 400,000 cells...leukocytes. The file shared also contains information about the labeling pattern of several cell lines using...Laflamme, C., Virk, H., NeuroSGC/YCharOS/EDDU Collaborative Group, & ABIF Consortium. (2024). A guide to...

  4. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...million views from 119 different countries (Figure 4). Four of the five most popular videos are protocol...other videos on Addgene’s YouTube channel.   Figure 4: Global viewership of the Addgene YouTube channel,...a less tangible form: knowledge and information about the materials in our repository. We started to increase...by making it easier to find materials and learn about them. As early as 2007, we began grouping similar...professors, stopping by to say, “Everything I know about virus, I learned from Addgene,” or “I learned to...seeing our resources shared via social media, on collaborators’ websites, or as a recommendation in a Reddit...updating and expanding our website, we’ve learned about the importance of smaller, systemic changes, like...

  5. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...and AAV1 are the most requested serotypes (Figure 4), our catalog includes vectors in a variety of serotypes... and the systemic capsid PHP.eB.   Figure 4: A) Cumulative AAV preps for the seven most popular...all, a delivery method. I would be remiss to talk about them without looking at what they’re delivering .... But in the years since, we’ve learned so much about what it takes to make these viral vectors not only...., 2016), video, educational resources, and collaborations, and will continue to do so, in the hopes that...leave a comment; or take our AAV survey. Learn more about GCaMPs and other tools developed through the GENIE...

  6. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...conservative estimate. As a graduate student, I drank 3-4 cups of coffee a day, and I had colleagues who were...learn about all the amazing research being done with plasmids. I heard an impressive talk about the use...pulls off such an inspiring, thought-provoking, collaborative event – all while keeping the party going! At...scientists we've spoken with are excited and passionate about making such tools open-access. Though it is time...

  7. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...and isolated.  Following a gating strategy Figure 4 shows a gating strategy from a published experiment...are very close to each other.        Figure 4: Gating strategy used in Barlow-Anacker et al., 2017...series of one- or two-marker plots, like in Figure 3 above, until the population of interest has been successfully...  Of course, there are many more things to learn about when diving into flow cytometry data. There’s different... ways to use FSC and SSC, as briefly alluded to above. There’s many variations in how one can gate, select...

  8. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...PMID: 19396156. PubMed Central PMCID: PMC3655711. 4. Gronostajski, R M, and P D Sadowski. 1985. “The FLP...and Removed at Persistent Sites In Vivo.” Neuron 89(4): 756–69. PubMed PMID: 26853302. PubMed Central PMCID... on the Addgene Blog Learn More about FLEX-vectors Learn More about Cre-Lox Check out Retrograde AAVs...expression have been developed. Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and...

  9. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...mice via adeno-associated viruses. Nat Biomed Eng 4:97–110 . https://doi.org/10.1038/s41551-019-0501-5...Commun 10: .https://doi.org/10.1038/s41467-018-08224-4  Swiech L, Heidenreich M, Banerjee A, Habib N, Li ...Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR enzyme Cpf1 ... and specific? The two AAV strategies described above showed successful target modification, indicating...

  10. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...might be contextual fear conditioning (reviewed in [4]). Contextual fear conditioning is a behavioral experiment...PMID: 23473324. PubMed Central PMCID: PMC3595120. 4.Mattis, Joanna, et al. "Principles for applying optogenetic...of the important considerations you should think about when developing optogenetics behavioral experiments...scientific research is that the field benefits from collaboration and the exchange of ideas. The rapid spread ...versatility and standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this...

  11. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

    Type
    Blog Post
    ...standardized, disciplined, and open techniques. Chapter 4: Research projects underway in the Ellis lab including...synthetic yeast chromosome. Listen to learn more about Dr. Ellis, synthetic biology, synthetic chromosomes...Additional Resources from the Addgene Blog Learn about the Synthetic Biology Education Nonprofit BioBuilder...BioBuilder Plasmids 101: Golden Gate Cloning Learn About Working at a SynBio Startup in Our Interview with...

  12. Addgene's Website Update

    Type
    Blog Post
    ... a fresh new friend: Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day ...straight from the new search bar, but the menu just above it can help you quickly and easily navigate to educational...down? You’ll find Blugene, as well as information about Addgene, our mission, links to our deposit, request...

  13. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ...: How did you end up becoming a graduate student? 4:50 - 8:00 : How did you approach doing rotations? ...graduate school community to help keep you excited about coming into lab even when things get tough in the...

  14. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...description of the experimental conditions.     Figure 4: A screenshot of the materials and methods section... antibody worked under the conditions described above, and a short description of how the antibody performed...

  15. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ... viruses in a BSL-2 lab instead of a BSL-3 or BSL-4 lab. For more details, check out this review article... with a deletion-mutant rabies virus. Nat Methods 4:47–49 . https://doi.org/10.1038/nmeth999 Wickersham... also toxic at high concentrations as described above and is inactivated by the complement system in serum...

  16. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...preferably near the 5’-end) and that also generates 4 bp overhangs. You can then use the Klenow fragment...Science 239 (1988): 487-491. PubMed PMID: 2448875. 4. Xia, Yongzhen, et al. "New insights into the QuikChangeTM... Brush up on Your Cloning Techniques Learn More about Plasmids in Our Plasmids 101 Series Use CRISPR to...

  17. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...with the crRNA, (3) three transposase proteins, and (4) the donor DNA. By using a multi-spacer CRISPR array...Using an Engineered CRISPR/Cas System. ACS Synth Biol 4:723–728 . https://doi.org/10.1021/sb500351f Dong C...diverse bacteria with Mobile-CRISPRi. Nat Microbiol 4:244–250 . https://doi.org/10.1038/s41564-018-0327-...CRISPR Featured Topic Page Learn more about Cpf1 Find out about CRISPR Multiplexing Resources on Addgene.org...coli and other bacterial species. Read on to learn about the tools available for bacteria and some of the...that can be used in this way. As described in the above CRISPRi section, the Marraffini Lab published a ...as a collection of organisms, their genes, and metabolic processes, occupying the gut. Many human diseases...

  20. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    ... translational. Ex: the discovery of GFP. Chapter 4: Goals of the Center for Regenerative Meidince - Understanding...promote scientific sharing. Listen to learn more about Darrell, his research, and his focus on open science...Medicine and in the Department of Pathology and Laboratory Medicine. He attends in the Medical Intensive...NIH-funded Principle Investigator in the Kotton Laboratory and is the founding Director of the Center for...Podcast.     Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids...Plasmids from the Kotton Lab Learn more about Stem Cells ...
Showing: 161 - 180 of 397 results