Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 450 results for: abo.5

Showing: 161 - 180 of 450 results

  1. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...Plasmids and Chromosomes. Microbiol Spectr. 2014 Oct;2(5). doi: 10.1128/microbiolspec.PLAS-0025-2014. PMID:...concatemers in mammalian cells. J Gene Med. 2009 May;11(5):444-53. doi: 10.1002/jgm.1310. PMID: 19263463. Ribeiro...multicopy plasmid stability. Mol Microbiol. 1998 Sep;29(5):1137-45. doi: 10.1046/j.1365-2958.1998.01012.x. PMID...colonies using one of the detection methods described above can help ensure the isolation of the monomeric version...plasmid DNA production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes...

  2. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...implants have been reported by several groups [4, 5, 6]. These have the obvious advantage of not requiring...PMID: 23580530; PubMed Central PMCID: PMC3769938. 5. Iwai Y, et al. A simple head-mountable LED device...distance so ideal implant placement should be about 0.4-0.5mm above the site of your stimulation. The scatter...scatters a great deal once it enters tissue, placement above the site of stimulation guarantees that the majority...instead). Laser stimulation         In the video above, Channelrhodopsin 2 (Chr2) has been expressed in...These lasers are relatively cheap and will run you about $2-3,000/laser. However, an important caveat is ...output of the laser must be controlled. As discussed above, each opsin variant has slightly different activation...

  4. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...antibodies against some AAV types, AAV1, 2, 3 and 5 are thought to be as high as 70% among the human population...therapy 10.11 (2003): 964-976. PubMed PMID: 12756417. 5. Chan, Ken Y., et al. "Engineered AAVs for efficient...Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control Process...viral infections. She is particularly passionate about HIV cure research and science communication.     ...

  5. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...Science Rapper Tom McFadden Learn about SITN's work at DayCon 2015 5 Reasons to Use Reddit for Science...supporters of science communication. To help you think about ways to effectively communicate your science, we... of programs SITN runs and learn how they think about communicating science. Listen to the interview ...

  6. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    ...below! Additional Resources on the Addgene Blog 5 Science Rap Videos and Their Creator: Tom McFadden...researchers time by quickly relaying key points about an unfamiliar topic or lab process. Along with our...goals, or to answer any questions you might have about Addgene. The page is divided into three main sections...a variety of roles at Addgene.  You’ll hear all about their career paths, future goals, and get some useful...

  7. dTAG - You're it!

    Type
    Blog Post
    ...dTAG-13 at doses as high as 20 µM (see Figures 3 and 5 in Nabet et al., Nature Chemical Biology for examples...degradation.. Nature Chemical Biology. May 2018. 14(5): 431-441. PubMed PMID: 29581585. This reference ...degrade any protein. We are particularly excited about the dTAG system as we can use it to evaluate the...

  8. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...requirement. For instance, for Casɸ-2 the PAM sequence is 5’-TBN-3’ (where B is G, T, or C). The authors demonstrate...new YFP variant, mGold. mGold was found to be up to 5-fold more photostable and as bright to mVenus, the... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's...

  10. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...nm) BD Horizon™ BB515BD Horizon™ PE-CF594Pe-CyBD™5 PEPE-Cy7 FITC Alexa Fluor 488PerCP-CY5.5 PerCP...example, I will describe a basic panel (Table 2, Figure 5) for a hypothetical experiment in the lab. Let's say...FITC channel CD45 BV421   Figure 5: Emission spectra of the fluorophores used in our ...spectrum is located in a range of wavelengths of about 380–700 nm. Photons of lower wavelengths are higher... 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If we were to use a laser that excites at...optimal excitation happens at higher wavelengths of about 750 nm. Source: https://www.bdbiosciences.com/en-us... we start building your first panel, we'll talk about one last principle of color emission. As you can...

  11. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...surgeries for viral injections can be found here [5, 6]. Opsin transgenic mice Transgenic mouse lines ...10.1038/nmeth.1808. PMID: 22179551; PMCID: PMC4165888. 5. Harris JA, et al. Adeno-associated viral vectors ...activation and silencing. Nature neuroscience. 2012;15(5):793-802. doi: 10.1038/nn.3078.  PMID: 22446880; PMCID... electrophysiology data too but that’s what collaborators are for). For example, if you study neural pathways...intended brain region and/or cell type (I’ll talk more about this in detail later). But what are the different...of your opsin, but keep you eyes open for posts about the next steps in the coming weeks. Expression of...Addgene. See the Deisseroth lab homepage for details about types of constructs available, instructions for ...

  12. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...other options. Commonly used Cpf1 variants, with a  5’ TTTV PAM, improve targeting in AT-rich loci or genomes...activity. Surprisingly, off-target effects across 5 gRNAs with NGG PAM sequences were actually lower for...Blog Learn about Engineered Cas9 PAM Variants Read about CRISPR Base Editing Learn about Homology Directed...editing specificity. We’re excited to learn more about xCas9 - here’s what we know so far! Why do we ...point mutations, also has a strict editing window about 15 nt upstream of the PAM. Even if there’s a PAM...in mammalian cells, finding xCas9 3.7 displayed about the same editing rate as SpCas9 at an NGG PAM. When...are exciting, there’s a lot we still don’t know about xCas9. “I’m not 100% sure xCas9 is going to be flat...

  13. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ... to limitations in light penetration mentioned above (5). Thick static samples Ex: Fixed tissue sections...providing fast imaging along with optical sectioning (5). One such example is the lattice lightsheet that ... Enzymol 361, 1-33 (2003). PubMed PMID: 12624904. 5. Power RM, Huisken J. A guide to light-sheet fluorescence...call Epi-fluorescence microscopes. (See left image above for an example image). Point Scanning Confocal Microscopy... plane to reach the detector and all light from above or below is excluded. Parallelized Confocal Microscopy...sectioning. Point scanning confocal microscopes (see above) often provide the highest quality images for this...

  14. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...Additional Resources Quick Guide to All Things Lentivirus 5 Tips for Troubleshooting Viral Transductions Getting...Addgene.org Check out our Viral Service Learn more about our Viral Production Process Find other Viral Vectors...

  15. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    ...being receptive to those who report to you. Chapter 5: The types of science that Crystal works on at Novartis...therapeutics at Novartis - listen to learn more about Crystal’s exciting career path, to gain insight ...Chapter 2: What's a lab group in industry? The collaborative nature of working in industry and how delegating...metrics for success. The importance of thinking about the big picture and not just the next experiment...resources in our Mentoring for Scientists Series Learn about Turning Your PhD into a Career with Joanne Kamens...

  16. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ... the desired gRNA target sequence, as well as the 5’ and 3’ homology arm repair template (Fig. 1, Step...of the same type will produce the same unique SapI 5’ overhang, different combinations of connector and...tagged gene. Most insertions can only be detected by labor-intensive processes like PCR or by evaluating visual...repair after a CRISPR/Cas9 induced break. Learn more about these constructs and the cell lines they’ve been...Additional Resources on the Addgene Blog Learn about Fluorescent Protein Timers Check out Photoactivatable...

  17. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...contaminant we mixed into the original AAV sample at 5%. Detecting 5% contamination is a good start, but to determine...viral genome sequencing (VGS). Why should you care about viral quality control? AAV vectors are produced ... let us know if you have any specific questions about viral DNA NGS you’d like us to discuss. We are also...or at [email protected] to answer your questions about our quality control process! The images in this...

  18. Rabies and Neuronal Tracing

    Type
    Blog Post
    ... Neurovirol. 2005;11:82–87. PubMed PMID 15804965. 5. Nassi JJ, Cepko, CL, Born RT, Beier KT. Neuroanatomy... midbrain dopamine neurons. Neuron. 2012 Jun 7;74(5):858-73. Pubmed PMID 22681690.  8, Wickersham IR, ...connected to the neck. Shortly after, we learned about organs and the jobs they perform. This became foundational...part of the answer to many biological questions about the brain. To address this, molecular tools to map...

  19. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... quantitative PCR. Expert Rev Mol Diagn. 2005 Mar;5(2):209-19. doi: 10.1586/14737159.5.2.209. PMID: 15833050...resistance-1 gene (pfmdr1). J Parasitol. 2011 Oct;97(5):939-42. doi: 10.1645/GE-2792.1. Epub 2011 May 9. ...controls must be run in triplicate.  The nice thing about qPCR is that since it measures relative quantification...number, albeit within a range of RQ values as noted above.   In both applications, results can be affected...

  20. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ... in the absence of genome editing (Fig. 2 and 3) (5, 3). Figure 2: Gene activation with nuclease-competent...PMID: 23287722. PubMed Central PMCID: PMC3712628. 5. Dahlman, James E., et al. "Orthogonal gene knockout...of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing scientific... Listen to Our Interview with Alex Chavez Learn about Sequencing Options for CRISPR Genotyping Cas9 Activators...
Showing: 161 - 180 of 450 results