We narrowed to 344 results for: ada
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TypeBlog Post...support entry of required metadata, with the option of adding additional metadata in the repository after...
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Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post...While selfish in nature, these systems have been adapted by researchers for many uses when plasmid retention... PMID: 8650219. PubMed Central PMCID: PMC39188. Kamada, Katsuhiko, Fumio Hanaoka, and Stephen K. Burley... -
To Codon Optimize or Not: That is the Question
TypeBlog Post...the late 1980s, scientists even created a codon adaptation index (CAI) that is based on the codon usage ...10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post...reporter, and neomycin resistance reporter. Image adapted from Katti et al., 2020. As a proof of concept...Gaudelli et al., 2017). GO is thus a flexible and adaptable tool to identify and enrich for base-editing events... -
Bioinformatics at Addgene
TypeBlog Post...have been developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting...Java, R, etc.) in our pipeline. Crucially, this adaptable property gave us the freedom to use multiple open-source... -
Transferable Skills Guide: Creativity
TypeBlog Post...your problem. In a study outlined in Originals by Adam Grant, a creativity expert at Stanford conducted... with the group (because they will fight more adamantly for their solution), selecting a devil’s advocate... -
Hot Plasmids - November 2023
TypeBlog Post...trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023 bioRxiv (A) and ...:GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0... -
Hot Plasmids: Fall 2024
TypeBlog Post...ubiquitination substrate Ikaros (IKZF1ZF2) (B). Image adapted from Kroupova et al., 2024 under CC BY license....design and studies of protein ubiquitination and degradation. Find the CRBNmidi plasmid here! Kroupova... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...such that prior formats are no longer supported/readable. Is the author or their host institution expected...Research Institute at Mount Sinai Hospital, Toronto, Canada where he studies protein-serine/threonine kinases... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...twist since it relies on the sequential nicking, degradation, and PCR-generation of a mutant version of the...Natarajan, Chandrasekhar, et al. "Epistasis among adaptive mutations in deer mouse hemoglobin." Science 340.6138... -
Hot Plasmids: Summer 2024
TypeBlog Post...structure of the H1.8-GFP-bound nucleosome. Images adapted from Arimura et al. 2024 under CC-BY-NC-ND 4.0 ...3D map with 3.1 Å global resolution (E). Figure adapted from Abe & Lim, 2024 under CC-BY-NC-ND 4.0 license... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...non-integrating transgenes. Created with BioRender.com; adapted from Banasik and McCray, 2010. These episomes...Chatters, S. J., de Ridder, D., Gilmour, K. C., Adams, S., Thornhill, S. I., Parsley, K. L., Staal, F.... -
Hot Plasmids: Spring 2025
TypeBlog Post...Rising τ, and Decay τ of the three sensors. Image adapted from Aggarwal et al. 2025 under a CC-BY-NC-ND 4.0...displaying characteristic PV basket cell morphology. Adapted from Furlanis, Dai, Leyva Garcia et al. 2024 bioRxiv... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...straight-forward, concise explanation of how to adapt our engineered devices, recombinase-based state ...not expressed in each state. To help researchers adapt GRSMs for their research, we provide MATLAB-based... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...you are working with RNAs, which are prone to degradation by the omnipresent RNase. You may want to declare...doi.org/10.1038/nbt.3026 Gurumurthy CB, Takahashi G, Wada K, Miura H, Sato M, Ohtsuka M (2016) GONAD: A Novel... -
Finding Your Perfect Job After University
TypeBlog Post... culture, and also needed to be resourceful and adapt. By the end of my three months, I fully understood...companies which have more bureaucracy and cannot be as adaptive. I was also surprised to learn that I enjoy ... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post... editing methods, other Cas proteins have been adapted for use in C. elegans. In 2017, the Korswagen lab...doi.org/10.17912/DDVH-BG64 Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013)... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...Lavis Lab (Grimm et al., 2017). These dyes were adapted into ligands for HaloTag® and are currently offered...doi.org/10.1128/spectrum.03690-23 Mahmoudian, J., Hadavi, R., Jeddi-Tehrani, M., Mahmoudi, A. R., Bayat,... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...functions more similarly to CRISPR/Cas9 systems adapted for eukaryotes in that Cas9-mediated cleavage induces...addition of a uracil DNA glycosylase inhibitor and degradation tag on the fusion protein to limit the activity... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post... the balance of biogenesis and mitochondrial degradation in vitro and in vivo. Merged green and red ...long life-cycle of a protein from expression to degradation. Chemical approaches may require expensive peptides...