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We narrowed to 332 results for: cloning

Showing: 161 - 180 of 332 results

  1. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...researchers to insert targeting oligos with minimal cloning required. As with other model systems, multiple...containing vectors ready for Golden Gate mediated cloning of your gRNA of interest. Figure 1: Simplified...

  2. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...cell lines is to transfect, select, and isolate clones. This procedure is cumbersome and inefficient, ... somewhere between one hundred and one thousand clones to find a cell line with the edit of interest. ...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 KB SpCas9...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...annealed oligos (design is indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold...annealed oligos (design is indicated below) can be cloned into the CRISPR array. The oligos are designed ...activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop...Hygro resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop...for insertion of guide sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome...

  4. Ligation Independent Cloning

    Type
    Protocol
    ... Protocols Ligation Independent Cloning Ligation Independent Cloning You may also ...Independent Cloning (LIC) obviates the need for the time-consuming ligation step of traditional cloning methods... Independent Cloning Protocols...also like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation Independent...methods. In traditional cloning, base-pairing in the short overlapping regions (usually 4 bp) does not provide... restriction sites from the final product (no "cloning scars"). A “stuffer” sequence allows for electrophoretic... and may provide counter-selection for negative clones as in the example shown here. The manufacturer ...

  5. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Additional Cloning Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol...provided as empty backbones that utilize Golden Gate cloning to insert the desired guide RNA sequence by digestion...Protocol (PDF, 237 KB) Dual guide cloning: Brzezinski Lab Dual Guide Protocol (PDF, 990 KB) Plasmid and Sequence...

  6. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...Video Restriction Cloning Assemble plasmids by restriction digest and ligation Cloning by PCR Generate ...single reaction Ligation Independent Cloning (LIC) Scarless cloning with Type II restriction enzymes and...and T4 polymerase pLKO.1 - TRC Cloning Vector Cloning protocols for using the pLKO.1 vector, a backbone...can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations... the Lab Bench Basic Molecular Biology Plasmid Cloning Virus Antibodies Intro to the Lab Bench Introductory...tissues or cells without a commercial kit Plasmid Cloning Protocols for constructing and analyzing your plasmids...restriction sites by PCR Modification by Annealed Oligo Cloning Add a short stretch of DNA to a plasmid Gibson ...

  7. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ... mutant clones from watermelon have been codon-optimized for expression in bacteria and cloned into an...region of MDH. For detailed clone information, please see the MDH Protein Clone Information Sheet, available...malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate research experience...IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus of MDH and, if indicated on the...

  8. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...PCR - or restriction enzyme -based cloning to test individual clones before use of more expensive forms...digested with restriction enzymes common to multiple cloning sites, will result in similar sized bands, thus...Insert Orientation by Restriction Digest If you clone an insert into a vector by a single restriction ... enzyme, you will need to verify that it has be cloned in the correct orientation - this can be done by...restriction digest. Although this is never an ideal cloning strategy, sometimes it cannot be avoided. If you...therefore the only thing left to do is identify the clone(s) in which the insert is in the correct orientation... want to know how to differentiate between two clones that differ only in the orientation of the insert...

  9. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning untagged proteins...Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen lentiviral backbone for cloning and expression ...may contain tags or fusion proteins, a multiple cloning site (MCS), an inducible promoter, and/or a selectable...with C-terminal mTagBFP2 tag pNIC-GST-TEV-GG - Cloning of target gene with N-terminal GST tag and TEV ...for bacterial expression, Ligation Independent Cloning (LIC) Also see our Bacterial Expression collection...zebrafish insulin promoter vector with multiple cloning sites Browse our full Fluorescent ...thioredoxin) Protein purification pNH-TrxT - LIC cloning in bacteria with N-terminal His-TrxA-TEV tag NusA...

  12. Microbiology Resources

    Type
    Collection
    ...- Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning System - Geertsma...Parts Kit : Molecular cloning for E. coli - Densmore Lab EcoFlex MoClo : Modular cloning for applications ... and Dutzler Labs EasyClone 2.0 Yeast Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt...Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology. CRISPR Resources...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...

  13. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ...Changing a Multiple Cloning Site (MCS) Adding short tags - ex: HA or Flag Cloning shRNAs Background For...using oligo overlap cloning. Protocols... Protocols Plasmid Modification by Annealed Oligo Cloning Plasmid Modification...Modification by Annealed Oligo Cloning You may also like... Restriction Digest of Plasmid DNA DNA Ligation Bacterial...Bacterial Transformation Summary Oligo overlap cloning can be used anytime you need to add a short stretch...NdeI, PacI, AscI, and MfeI. With oligo overlap cloning, you can design a set of oligos containing your...design overlapping oligos that once annealed can be cloned directly into the overhangs generated by restriction...

  14. Antibody Plasmid Collection

    Type
    Collection
    ...well as by our trusted partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies...synthetically produced recombinant antibodies, created by cloning antibody components into plasmids and expressing...Monoclonal antibodies are derived from an individual clone targeting a single antigen. An example of R-mAbs...and their applications in our Antibody Guide ! Cloning and Expressing Antibodies The following table highlights...mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies. Sci Rep... Rep. 2014 Jul 30;4:5885. Andrew Beavil Cloning and mammalian expression of mouse and human IgG monoclonals...

  15. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...pLKO.1 TRC-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning Vector A...these bases are important for cloning the oligos into the pLKO.1 TRC-cloning vector. Forward oligo: 5’ CCGG...GTATAGCTTAACGTAGGCATT 3’ Back to Top C. Cloning Oligos into pLKO.1 The pLKO.1-TRC cloning vector contains a 1.9kb stuffer...digests. Digest pLKO.1 TRC-cloning vector with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep...Sequence of pLKO.1 TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning vector. The vector...Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting... Protocols pLKO.1 Protocol pLKO.1 - TRC Cloning Vector Addgene Plasmid 10878. Protocol Version ...

  16. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...Options”, including adding adapter sequences for cloning purposes.  Press run and wait for the generation...this purpose.  The gRNA oligos are subsequently cloned into your vector of choice using the adapter sequences...

  18. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...plasmids The transfer plasmid is the vector you clone your gene of interest into and is an important feature...Lentiviral production and transduction Once you have cloned your gene of interest into lentiviral transfer ...

  19. Synthetic Biology - Overview

    Type
    Collection
    ...Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly...Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification...

  20. TALEN Plasmids and Kits

    Type
    Collection
    ...336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one cloning step rather...Stephen Ekker Golden Gate TALEN 2.0 Golden Gate cloning method. Validated in multiple organisms. Dan Voytas...Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang... Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit Hornung Musunuru...plasmids sequence positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting in shorter...RR) used are obligate heterodimers that require cloning of left and right TALEN monomer proteins into opposite...enhancers in mammalian cells. These plasmids allow for cloning DNA fragments encoding TAL effector repeat arrays...
Showing: 161 - 180 of 332 results