Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 322 results for: cloning

Clear keywords
Showing: 161 - 180 of 322 results

  2. Plant Plasmids and Resources

    Type
    Collection
    ...as the promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI Synthetic... vectors. ID Kit Description Cloning Type PI 1000000036 GreenGate Cloning System Plasmids for rapidly ...Stuttmann 1000000233 Joint Modular Cloning (JMC) Toolkit Modular cloning chassis and set of functional parts...Explore plant-optimized plasmids for cloning, gene expression, reporter assays, genome editing, gene ...Collections Plant Plasmids Plant Plasmids and Resources Cloning & SynBio Genome Editing Gene Silencing Reporters... general and species-specific gene expression. Cloning and Synthetic Biology Expression Vectors The table...Kit Plant synthetic biology kit for GoldenBraid cloning and multigene engineering, including CRISPR/Cas9...

  8. CRISPR References and Information

    Type
    Collection
    ...gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for Cas9 orthologs... Cas9 PDF, 66.8 KB Fujii gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo... Fly: gRNA cloning pAc-sgRNA-Cas9 PDF, 169 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF,...Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 KB Sontheimer gRNA design and cloning All-in-one ... helps design (10 different prediction scores), clone (primer design), and evaluate gRNAs, as well as ...plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; ...PDF, 110 KB Goldstein Nematode: gRNA design and cloning Cas9-sgRNA construct PDF, 364 KB Goldstein Nematode...

  9. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Additional Cloning Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol...provided as empty backbones that utilize Golden Gate cloning to insert the desired guide RNA sequence by digestion...Protocol (PDF, 237 KB) Dual guide cloning: Brzezinski Lab Dual Guide Protocol (PDF, 990 KB) Plasmid and Sequence...

  11. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...monoclonal antibodies are derived from a single B cell clone. One common way of producing monoclonal antibodies...freeze hybridoma cells before culturing or they can clone the genes from the hybridoma into a plasmid to create...

  13. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...my lab we do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made...

  16. Zhang Lab CRISPR Page

    Type
    Collection
    ...Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 KB SpCas9...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...annealed oligos (design is indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold...annealed oligos (design is indicated below) can be cloned into the CRISPR array. The oligos are designed ...activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop...Hygro resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop...for insertion of guide sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome...

  17. Microbiology Resources

    Type
    Collection
    ...- Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning System - Geertsma...Parts Kit : Molecular cloning for E. coli - Densmore Lab EcoFlex MoClo : Modular cloning for applications ...Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology. CRISPR Resources...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ....0 Yeast Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt Lab MultiSite Gateway Kit : ...

  18. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning untagged proteins...Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen lentiviral backbone for cloning and expression ...may contain tags or fusion proteins, a multiple cloning site (MCS), an inducible promoter, and/or a selectable...with C-terminal mTagBFP2 tag pNIC-GST-TEV-GG - Cloning of target gene with N-terminal GST tag and TEV ...for bacterial expression, Ligation Independent Cloning (LIC) Also see our Bacterial Expression collection...zebrafish insulin promoter vector with multiple cloning sites Browse our full Fluorescent ...thioredoxin) Protein purification pNH-TrxT - LIC cloning in bacteria with N-terminal His-TrxA-TEV tag NusA...

  19. Synthetic Biology - Overview

    Type
    Collection
    ...Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly...Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification...
Showing: 161 - 180 of 322 results