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We narrowed to 494 results for: control

Showing: 161 - 180 of 494 results

  1. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...donor templates and sequencing primers for quality control. At such a scale, automation is crucial, both for... to avoid re-cutting when needed. It gives you control over your data. CrispyCrunch keeps a record of ...repair outcomes, which is important for quality control. The only ones we found that worked with NGS data...

  2. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...policy gives us time to complete necessary quality control and legal approvals so the plasmids are ready for...start out with this hold! You will always have control over when your plasmids are distributed. Next month...

  3. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ... your preparation for a long time. Tris buffer controls the pH, while the EDTA chelates any divalent cations...of using a Tris buffer is that it allows better control of the pH preparation stabilizing the plasmid, ...

  4. Hot Plasmids - August 2020

    Type
    Blog Post
    ...new tool for synthetic biology uses CRISPRi to control gene expression. This includes a CRISPRi-based ...affinity), as well as GRAB_NEmut as a negative control. Find the sensors at Addgene! New yellow fluorescence...AAV5 and AAVrg. Find these AAVs at Addgene New controls, biosensor, chemogenetic and optogenetic AAV for...

  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ... and negative (gray) droplets. The no template control wells should have very few positive droplets. In...positive copy per microliter in the no template control wells. You’ll also notice a reduction in the number...assay, so cross contamination into a no template control (NTC) well is common. Here are some steps you can...

  6. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...to the neuronal networks involved in directly controlling muscle activity and coordinating movement, several...optogenetics to support these existing findings by controlling neurons in the mPFC with light. Activation of...inhibited by fear/anxiety. On the other hand, the control group preferred staying in the dark compartment...

  7. Bioinformatics at Addgene

    Type
    Blog Post
    ...laboratories and associated publications, but our quality control (QC) standards have evolved over the years to keep... ability to re-use or borrow existing code and control how the various pieces were connected, as this ... for Bioinformatics AnalysesAAV Vector Quality Control: Going the Extra Mile with NGS...

  9. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...standpoint, a plasmid with all four genes and necessary control elements for each would be relatively large. Cloning...separate plasmids allows researchers to partially control expression levels of each gene by altering the ...genes (either viral genes or minigenome) are under control of a T7 RNA polymerase promoter sequence. Thus,...

  10. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...accounted for using appropriate replicates and controls (see Tips below), and by validating leads with...reporter gene activation. Including a prey-only control also provides a baseline level of reporter activation...that are known to bind serves as a good positive control to ensure that your setup works. The last thing...

  11. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...possible to bring all aspects of our lives under control? Balance is a myth and should not even be the real... don’t have children, so my life will be under control.” Think again. Maybe you’ll need to care for an...to do most tasks – try to resist the impulse to control by insisting things always be done your way. Be...

  12. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...anticipated outcomes? What are your controls? Will these controls enable you to interpret your results...figures are missing? For example, are additional controls necessary? As you build your story, are additional...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

  13. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...sometimes observe something odd in our uncut DNA control: a band or two appear on an agarose gel at notably...agarose gel Running undigested DNA on a gel as a control when performing a diagnostic digest is a simple...sequencing of our plasmid samples during our quality control process. Since MiSeq involves sequence assembly.... 2006 Sep;4(8):38-40. Summers D. Timing, self-control and a sense of direction are the secrets of multicopy...

  14. Tags and Other Markers

    Type
    Collection
    ...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...

  15. Neuroscience

    Type
    Collection
    ...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...

  16. Synthetic Biology - Metabolism

    Type
    Collection
    ...Biofuels Natural Product Pathways Efflux Pumps Flux Control Metabolism Plasmids Search the table by keyword...

  17. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ... A fluorescent probe that releases an internal control Another autophagy biosensor is the GFP-LC3-RFP-LC3ΔG...it remains in the cytosol acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio...

  18. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...is. Thankfully, through the use of engineering controls — changes made to the virus that eliminate or ...viral vectors to reproduce only in experimentally controlled conditions, making them far safer to work with...

  19. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...function of Your Gene Of Interest (YGOI) in a controlled environment under various conditions. The first...great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial expression...
Showing: 161 - 180 of 494 results