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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 494 results for: control

Showing: 161 - 180 of 494 results

  1. Deisseroth INTRSECT Collection

    Type
    Collection
    ...recombinase recognition sequences enables directional control of the portion of the ORF that is sandwiched between... INTRSECT recombinases, viruses, and important controls to consider as part of experimental design. How-to..., Paninski L, Li B. 2017. The central amygdala controls learning in the lateral amygdala. Nat Neurosci...Meletis K. 2019. A hypothalamus-habenula circuit controls aversion. Mol. Psychiatry 24(9):1351-1368. PubMed...

  2. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...policy gives us time to complete necessary quality control and legal approvals so the plasmids are ready for...start out with this hold! You will always have control over when your plasmids are distributed. Next month...

  3. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ... your preparation for a long time. Tris buffer controls the pH, while the EDTA chelates any divalent cations...of using a Tris buffer is that it allows better control of the pH preparation stabilizing the plasmid, ...

  4. Worm Expression Resources

    Type
    Collection
    ...silencing in the germline. A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. - ...Hubbard Lab. Plasmids for the temporal and spatial control of gene expression in C. elegans by combining expression...

  5. mTOR Pathway

    Type
    Collection
    ...apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation. mTOR, a serine...Kris Wood) References mTOR signaling in growth control and disease. Laplante M, Sabatini DM. Cell. 2012...in cancer promotes the conditions needed for uncontrolled cell growth and proliferation, including an ...

  6. Bioinformatics at Addgene

    Type
    Blog Post
    ...laboratories and associated publications, but our quality control (QC) standards have evolved over the years to keep... ability to re-use or borrow existing code and control how the various pieces were connected, as this ... for Bioinformatics AnalysesAAV Vector Quality Control: Going the Extra Mile with NGS...

  7. Hot Plasmids - August 2020

    Type
    Blog Post
    ...new tool for synthetic biology uses CRISPRi to control gene expression. This includes a CRISPRi-based ...affinity), as well as GRAB_NEmut as a negative control. Find the sensors at Addgene! New yellow fluorescence...AAV5 and AAVrg. Find these AAVs at Addgene New controls, biosensor, chemogenetic and optogenetic AAV for...

  8. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ... and negative (gray) droplets. The no template control wells should have very few positive droplets. In...positive copy per microliter in the no template control wells. You’ll also notice a reduction in the number...assay, so cross contamination into a no template control (NTC) well is common. Here are some steps you can...

  9. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...to the neuronal networks involved in directly controlling muscle activity and coordinating movement, several...optogenetics to support these existing findings by controlling neurons in the mPFC with light. Activation of...inhibited by fear/anxiety. On the other hand, the control group preferred staying in the dark compartment...

  10. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...standpoint, a plasmid with all four genes and necessary control elements for each would be relatively large. Cloning...separate plasmids allows researchers to partially control expression levels of each gene by altering the ...genes (either viral genes or minigenome) are under control of a T7 RNA polymerase promoter sequence. Thus,...

  11. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...accounted for using appropriate replicates and controls (see Tips below), and by validating leads with...reporter gene activation. Including a prey-only control also provides a baseline level of reporter activation...that are known to bind serves as a good positive control to ensure that your setup works. The last thing...

  12. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...possible to bring all aspects of our lives under control? Balance is a myth and should not even be the real... don’t have children, so my life will be under control.” Think again. Maybe you’ll need to care for an...to do most tasks – try to resist the impulse to control by insisting things always be done your way. Be...

  13. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...sometimes observe something odd in our uncut DNA control: a band or two appear on an agarose gel at notably...agarose gel Running undigested DNA on a gel as a control when performing a diagnostic digest is a simple...sequencing of our plasmid samples during our quality control process. Since MiSeq involves sequence assembly.... 2006 Sep;4(8):38-40. Summers D. Timing, self-control and a sense of direction are the secrets of multicopy...

  14. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...anticipated outcomes? What are your controls? Will these controls enable you to interpret your results...figures are missing? For example, are additional controls necessary? As you build your story, are additional...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

  15. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...is. Thankfully, through the use of engineering controls — changes made to the virus that eliminate or ...viral vectors to reproduce only in experimentally controlled conditions, making them far safer to work with...

  16. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ... A fluorescent probe that releases an internal control Another autophagy biosensor is the GFP-LC3-RFP-LC3ΔG...it remains in the cytosol acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio...

  17. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...function of Your Gene Of Interest (YGOI) in a controlled environment under various conditions. The first...great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial expression...

  18. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...needed and when is it not? What are the proper controls needed to pressure-test your FRET assay to make...molecular distances (see Table). However, proper control experiments are needed to establish the dynamic...These experiments include producing a positive control construct where the donor and the acceptor fluorophore... each other on the same protein and a negative control experiment conducted on donor-only labeled protein...

  19. Tags and Other Markers

    Type
    Collection
    ...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...

  20. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...process, see our blog post on NGS plasmid quality control. NGS is generally cheaper if you have many samples...to sequence our packaged viral DNA for quality control. This gives us a faster turnaround time than short-read...Validation: seqWell Addgene’s Tips for Plasmid Quality Control Additional Resources on addgene.org Molecular...
Showing: 161 - 180 of 494 results