We narrowed to 453 results for: crispr

Catalytically inactive CRISPR plasmids for purification of target loci.

CRISPR plasmids for transcriptional repression of target genes.

CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs).

CRISPR plasmids for activating or upregulating gene expression in various model organisms.

Empty gRNA expression vectors for insertion of custom gRNA target sequences.

Find prime editor plasmids for (search and replace) genome editing.

From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently.

CRISPR nickase plasmids for the generation of single-strand breaks.

CRISPR plasmids that create double strand breaks in DNA.

Find CRISPR plasmids to study the regulatory networks involved in retinal development.

Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Learn how Cpf1 stacks up against Cas9 and NgAgo

The Doudna lab recently described a new, thermostable Cas9 variant, GeoCas9. Learn how you can use GeoCas9 in your next genome engineering experimetn.

Find out how you can use new CRISPR tagging plasmids from the Allen Institute for Cell Science to tag your cellular structure of interest.

Guest blogger Pooran Dewari presents results of an NgAgo survey he gave to some of the first NgAgo users. Find out how NgAgo compares to Cas9 so far.

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.

Researchers at the Wyss Institute for Biologically Inspired Engineering explain how you can use truncated gRNAs to regulate gene expression.