We narrowed to 209 results for: dap

1. Plasmids 101: Multicistronic Vectors

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   Blog Post

   ...disadvantages of the IRES element, scientists have adapted "self-cleaving" 2A peptides into their muliticistronic...

2. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...medicine. The parts we have right now are well adapted to work in prokaryotic bacteria, not in fungal ...

3. An Introduction to Adenovirus

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   ...cellular events, all recruit strong innate and adaptive immune responses that inactivate viral genomes...

4. Getting to Know Expansion Microscopy

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   ...model systems, paired with SRM techniques, and adapted for detecting nucleic acids as well as proteins...

5. CRISPR 101: RNA Editing with Cas13

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   ..., 2016) as a RNA-targeting CRISPR enzyme, they adapted Cas13b for precise RNA editing (Cox et al., 2017...

6. DNA Purification Without a Kit

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   ...or TE. The Bishop Lab’s glass milk protocol is adapted from the 1979 Patterson Protocol. When published...

7. A Guide to Starting Your Own Journal Club

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   ...group and topic. For example, it may be useful to adapt the second version (multiple presenter, figure by...

8. SciComm: Taking Science to Elementary and Middle Schools

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   ...formulated the presentation as follows (which could be adapted for any topic--not just CRISPR): Who am I? Where...

9. Tips for Screening with Yeast Two Hybrid Systems

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   ...Stynen B., Van Dijck P., Tournu H. A CUG codon adapted two-hybrid system for the pathogenic fungus Candida...

10. Hot Plasmids: Fall 2024

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    ...ubiquitination substrate Ikaros (IKZF1ZF2) (B). Image adapted from Kroupova et al., 2024 under CC BY license....

11. Antibodies 101: The Four ELISAs and When to Use Them

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    ...measure antibody levels, scientists have since adapted it for a host of different proteins and small molecules...

12. Plasmids 101: Stringent Regulation of Replication

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    ...Figure 2: Prokaryotic chromosomal replication. Adapted from BioRender template. Since DnaA binding...

13. Plasmids 101: Broad Host Range Plasmids

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    ...contains structural elements that are versatile and adaptable. Variation in ori structure allows plasmids to...

14. An Inside Look into Addgene's Dev Team

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    ...in iterative chunks, we have the flexibility to adapt and easily respond to changing needs of the teams...

15. Fluorescent Proteins 101: Aptamer Fluorophores

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    ...nucleotide-based ligand binders would prove to be highly adaptable tools. The key to aptamers’ flexibility is their...

16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ...While selfish in nature, these systems have been adapted by researchers for many uses when plasmid retention...

17. Plasmids 101: Codon usage bias

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    ...structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms often need...

18. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    ...non-integrating transgenes. Created with BioRender.com; adapted from Banasik and McCray, 2010. These episomes...

19. Antibodies 101: Normalization and Loading Controls for Western Blots

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    ...the gel (n=3). Data is presented as mean ± SEM. Adapted from Analytical Biochemistry, vol 440, Gilda, J...

20. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    ...Natarajan, Chandrasekhar, et al. "Epistasis among adaptive mutations in deer mouse hemoglobin." Science 340.6138...