We narrowed to 371 results for: fus

...cryopreserved hybridomas, immortalized cells created by fusing an activated B cell that produces the antibody ...light (VL) and variable heavy (VH) chain sequences. Fusion PCR was performed to create an amplicon that  was...

...components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating two MS2 RNA aptamers ..., which Konermann recognized as an ideal spot to fuse the activators instead of at the protein’s C terminus...

...parameters of the coupling system include: Wide fusion-tolerance — Click Biology tags should be similarly...organisms, targeted to particular compartments, or fused to other effectors from nanobodies to fluorescent...

...I suspected I knew at least one source of the confusion in our meetings. A lot of our LIMS planning involved...can help the team identify possible points of confusion in the future. Improving cross team communication...

...catalytically inactive cas9 (e.g. dCas9) has been fused to a variety of protein modules to repress or activate...consist of the GAL4 or ZIF268 DNA binding domains fused to either the hER or hPR ligand binding domains ...

... with soluble fluorescent proteins or localized fusion proteins and do not require dedicated plasmids ...performed on purified fluorescent proteins. To avoid diffusion, the proteins are (i) trapped in microdroplets...

...   Prior to the polyhistidine tag, alternative fusion tags were used, such as S. aureus’ Protein A (Moks... to scientists. If their protein of interest is fused to polyhistidine, then they can use an anti-HIS ...

... monitor this activity, Pedre Pérez’s biosensor fuses MPST to roGFP2. Because roGFP2 contains two cysteines...chemogenetic agonist inhibits the neuron (via hM4D). She fused fluorescent protein tags to the receptors to monitor...

...orthologs NmCas9 and St1Cas9. Each ortholog was fused to a different fluorescent protein to create three... recruit fluorescent proteins BFP, GFP, and RFP fused to the respective hairpin binding domain. Pairs ...

... as clear as you can be (there will always be confusion about this though) about the fact that containers...them to their contents might leave you sad and confused when a tricky procedure failed and one of your...

...particles are smaller and may be preferable if wider diffusion is required. On the other hand, lentiviral vectors...has to be slow to allow the viral particles to diffuse away from the injection site, and also to minimize...

...Once you input a sequence, SplitSet finds optimal fusion sites to split it into multiple fragments. You ...windows” you’d like the tool to use to identify fusion sequences for either a circular or linear assembly...

...varied as it can have important implications for the fusion protein effectivity), and a fluorescent protein...degradation the Ward lab used fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible...

...domain. Because ABE7.10 contains a heterodimeric fusion of wild-type TadA and TadA7.10, they designed one...developed a second set: The ABE8.x-d version carries a fusion of wild-type TadA and the evolved TadA region. ...

...(SP) to carry catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct...flexible. Like the xCas9 3.7 nuclease, xCas9 3.7 fused to third generation cytidine base editor architecture...

...then be fused to effector domains such as transcriptional activators or repressors. These fusion proteins...

...swapping and generating N and C terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, ...Vectors: High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants’, Bioscience...

...envisioned a two-component RNA editor: a Cas13 enzyme fused to an RNA adenosine deaminase (ADAR). Such a system...in RNA, creating a functional A->G change. They fused ADAR deaminase domains (ADARDD) to dPspCas13b, but...

...across a couple other terms that can cause some confusion. Paraformaldehyde: Many IF protocols call for...crossing cell membranes as they are not able to diffuse across natively. Permeabilization essentially punches...

...construct, 3 copies of the fluorescent protein were fused in-frame to a tethering partner, which directed ...enzyme choice is critical. May not support in-frame fusions of coding sequences Sladitschek and Neveu, 2015...