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We narrowed to 416 results for: iCre

Showing: 161 - 180 of 416 results
  1. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...neurons (-3mnx1/Hb9), pan neuronal (elavl3/HuC), microglial/macrophage (mpeg1.1), and astrocytic (gfap).This...transfer) and FLIM (Fluorescence Lifetime Imaging Microscopy) applications. To demonstrate the power of mCyFP1...variant, which could be compatible with two-photon microscopy using Ti-Sapphire lasers. pBAD-RDSmCherry1 is...
  2. Technical Design of a Western Blot

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    Blog Post
    ...protein in the femtogram range and another in the microgram range, they cannot be read with equal accuracy...is not compatible with sodium azide, a common microbial agent used in antibody buffers, which may be a...linear reaction rate and are compatible with antimicrobial agents. Chemiluminescence kits When activating...
  3. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...agarart from our lab @SILS_UvA 🧬🧫 Soon on display @Micropia! #SciArt #MerryChristmas #happyholidays2018 #DeckTheLab...ladiesinlabcoats used a pipette tip box and microcentrifuge tubes to make Eppi the red nosed reindeer. ...
  4. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...spatial, cellular, metabolic, and cytokine microenvironment that exists in vivo. Over the past five years...cell type, in more complex and physiological microenvironments. These screens have primarily been performed...
  5. Easing the Protein Purification Process with pCri

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    Blog Post
    ...pastoris. The improved secretory capacities of these microbes make them useful for producing difficult-to-fold...: a comprehensive review of affinity tags and microbial applications." Biotechnology journal 7.5 (2012...
  6. What's New in CRISPR - September 2019

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    Blog Post
    ...Cas protein, CasX, from metagenomic analysis of microbial DNA from groundwater. Now, they’ve recently published...different states of assembly from cryo-electron microscopy, the team reveal the mechanism behind its activity...
  7. Mapping the 4D nucleome with CRISPR/Cas9

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    Blog Post
    ...developed this technique using standard fluorescence microscopy, and are thus limited by its lower resolution...combination of this method with superresolution microscopy may improve the resolution, although background...
  8. Plasmids 101: Gene, enhancer, and promoter trapping

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    Blog Post
    ...poorly defined function (Wood, et al). In the microbial world, approximately 50% of predicted genes have...Vanni, C., et al., Unifying the known and unknown microbial coding sequence space. eLife. (2022) 11:e67667...
  9. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...on site courses with the American Society for Microbiology (ASM) Scientific Writing and Publishing Institute... The instructors are leaders in the field of microbiology with multiple high-profile publications. My ...
  10. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...result that you generated with a caption. For microscopy images, please include channel imaged, a color...color legend for probes/dyes/labels, type of microscope used, and objective used. If you did not generate...
  11. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ... for discriminating between neurons for light microscopy-based neuronal reconstruction. To achieve stochastic...aspects of sensory circuit development using light microscopy. Lab website: https://dn-en.med.kyushu-u.ac.jp...
  12. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ... mRFP and GFP fused to the autophagy-related microtubule-associated proteins 1A/1B light chain 3B, or ... GFP photoconversion Light sheet fluorescence microscopy Resources on Addgene.org Fluorescent protein...
  13. RUBY-Red Siliques

    Type
    Blog Post
    ...achieved much easier than by PCR or a fluorescent microscope. First, red CRISPR-positive transformants can...Carolina at Chapel Hill. He has a passion for microscopy and beautiful imaging. References and Resources...
  14. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...these drafts and for a few model organisms, Micropublication will accept negative data or publish brief...brief, novel findings. Most Micropublications contain one figure. If you aren’t there yet, write introductions...
Showing: 161 - 180 of 416 results