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Showing: 161 - 180 of 222 results
  1. Cloning

    Type
    Guide
    ...website for easy to use MoClo and Golden Gate cloning kits for your next cloning project. Golden Gate Cloning...
  2. CRISPR References and Information

    Type
    Collection
    ...plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Kits are also available (mouse or human libraries) PDF...opens in a new window) . GenET (Genome Editing Toolkit) (Link opens in a new window) is a library of various...
  3. COVID-19 Resources

    Type
    Collection
    ... Addgene's Protocol for RNA Extraction Without A Kit More Coronavirus Resources For more research related...available from Stanley Qi's lab. COVID-19 Diagnostic Toolkit Enzymes. (unpublished) Taq polymerase and MMLV ...
  4. CRISPR Library Amplification

    Type
    Protocol
    ...Purify plasmid DNA using the Qiagen HiSpeed Maxi Kit (one conical is its own Maxiprep). Critical Do not...
  5. Immunology Research Plasmids and Resources

    Type
    Collection
    ... Additional Resources Plasmid Kits and Collections Human Kinase ORF Kit : 558 distinct human kinases and...growth factor-like protein 2 - KITLG KIT ligand DKFZp686F2250, KL-1, Kitl, MGF, SCF, SF, SHEP7 KL klotho...Root . Multiple Lentiviral Expression (MuLE) System Kit : A modular and flexible system that can be used ...infection from the lab of Ian Frew . MXS Chaining Kit : From the lab of Pierre Neveu , the MXS-chaining...applications in mammalian systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites... inducible gene expression. Myristoylated Kinase Kit : Consists of 190 human kinases and kinase-related... Hahn , Matthew Meyerson , and David Root , this kit consists of 182 wild-type and 19 well-characterized...
  6. Antibody Guide

    Type
    Guide
    ... the membrane. Activate the HRP with a substrate kit. Image the membrane using either X-ray film in a ...
  7. Antibody Guide

    Type
    Collection
    ... the membrane. Activate the HRP with a substrate kit. Image the membrane using either X-ray film in a ...
  8. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Description PI Multiplex CRISPR/Cas9 Assembly System Kit Mammalian A system for constructing all-in-one expression... Cas9 from CBh promoter. Ventura A CRISPR/Cas9 toolkit for multiplex genome editing in plants Plant Plasmids...
  9. Cre-lox system

    Type
    Collection
    ...PhyBdeltaCreC and PIF6CreN, in middle entry vector (Tol2 kit) Zebrafish Eisenhoffer 131783 pTol2-CreLite CreLite...
  10. What's New in CRISPR - December 2019

    Type
    Blog Post
    ... CRISPRi toolkit for Burkholderia Silvia Cardona’s lab recently developed a CRISPRi toolkit for Burkholderia...this toolkit might be used in. Read the paper in ACS Synthetic Biology Find the CRISPRi toolkit SECURE...CRISPR-based toolkit for targeted genomic integration of fluorescent tags in neurons. Their toolkit named ORANGE...: CRISPRi in Burkholderia Base editors CRISPR toolkit for epitope tagging Lentiviral capsid-based Cas9...in bioRxiv Find Target-ACE   CRISPR/Cas9-based toolkit for epitope tagging in neurons Harold MacGillavry...neurons.  Read the paper in bioRxiv Find the ORANGE toolkit   Cas9/gRNA ribonucleoprotein delivery by lentiviral...
  11. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...Bee Microbiome Toolkit (BTK) to study bumble bee and honey bee microbiome. The toolkit consists of modular...Golden Gate Assembly. They built this toolkit upon the Yeast Toolkit by replacing the backbone vector’s ...Studying the bee microbiome using the Bee Microbiome Toolkit (BTK) Another system that lacks genetic tools is...used to study other microbiome systems. Find the toolkit for modular assembly of broad host range plasmids...engineering of bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids...
  12. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...that are sure to be enabled by this toolkit. For examples of the toolkit in action, see Cermak T, et al. ...Gateway-Compatible Toolbox is based on the original Tol2kit generated by the Chi-Bin Chien lab (Kwan et al....with TZAP Multiplexible plant genome engineering toolkit The Voytas Lab at the University of Minnesota recently...engineering across a variety of plant species. This toolkit includes TALEN and CRISPR nucleases, nickases, ...systems for multiplex gRNA expression. Expression of toolkit components can be controlled by a variety of promoters...cloning steps. The Voytas lab plans to update the toolkit frequently and has developed a website to aid in...
  13. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ... al. RNA. 2018 PubMed PMID:29109157. Nanobody toolkit to monitor retrograde transport at the cell surface...contributed by Marcy Patrick  Listen to the nanobody toolkit podcast segment Nanobodies are single domain antibody...retrograde transport from the cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP...related fluorophores. Find all of the nanobody toolkit plasmids here. Buser DP, et. al. PNAS 2018. PubMed...
  14. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    ...plasmid deposit please sign up here.        New toolkit for N or C terminal tagging of proteins for degradation...2016). Now, the Kanemaki lab has created a new toolkit of plasmids for the N- or C- terminal tagging of... (Yesbolatova et al., 2019) using CRISPR. This toolkit makes it easy to tag your protein of interest to...Yesbolatova et al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods, 2019. PubMed...
  15. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...is being organized and funded by someone else (KittyBiome has been incorporated as a non-profit entity ...how to start applying their skills to it. The Kittybiome project gives them access to rich and complex...something interesting and contribute it back to the kittybiome project, but the primary goal is learning in ...www.openworm.org/ Cat Microbiome Project Website: http://www.kittybiome.com/   ...
  16. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing in plants While there have been...nucleases. In doing so, they also created a modular toolkit for Cas-mediated genome engineering in plants. ...paper in bioRxiv Find the plant genome editing toolkit New Cas protein identified Jennifer Doudna’s ...
  17. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...CRISPR Corner New ready-to-use viral preps New toolkit for CRISPR-based genome editing in organoids By...members of the Rawlins Lab have compiled a new toolkit for performing genetics studies in organoids. The...together CRISPR-based approaches and organoid models, toolkits like this one provide new opportunities for investigating...
  18. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...applications. Despite the ubiquity of CRISPR/Cas toolkit, genome engineers continue to work diligently and...experimental successes and further develop the CRISPR/CAS toolkit by posting generic lab protocols, providing tips...
  19. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ... discussion on the way to the office or in the kitchen, while sipping an afternoon coffee in between experiments...used and could bring back a taste of that casual kitchen chatter, ultimately sparking new ideas. Social ...
Showing: 161 - 180 of 222 results