We narrowed to 286 results for: lum

1. Antibodies 101: Flow Cytometry Controls

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   Blog Post

   ...addition, the controls will help you save on sample volume, so you can use most of your samples for flow analysis...

2. Improved Plasmid Maps Powered by SnapGene

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   Blog Post

   ...cutters. You can also sort enzymes by clicking on column heads. Clicking on a particular restriction enzyme...

3. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   Blog Post

   ...and create ultrasound contrast. At a very low volumetric density and with a resolution of less than 100...

4. Clear and Accessible Writing

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   Blog Post

   ... at the top of the tube allows air to enter the column so the virus will flow out the bottom syringe."...

5. AAV Q&A with Tim Miles

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   ...  As always, you will need to discuss injection volumes with your IACUC committee.  Animal Models Q9: ...

6. GPCRs: How Do They Work and How Do We Study Them?

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   Blog Post

   ...be used to detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check ...

7. CRISPR 101: Non-Homologous End Joining

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   Blog Post

   ... lab.        Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology, the Dept. ...

8. RNA Extraction Without A Kit

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   ... a bunch of random solutions when all the spin columns run out. In this article, we cover the basics of...

9. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   Blog Post

   ...systems in their own research. Cyanobacteria, a phylum of photosynthetic bacteria, has increasingly been...

10. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post

    ...colonies (5-10) and grow them in a small culture volume (2 or 3 ml) containing the corresponding antibiotic...

11. Plasmid Tools for Microbiome Studies

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    Blog Post

    ...natural environment. Thus, the Harris Wang lab at Columbia University developed a method for genetically ...

12. Celebrating 15 Years of Scientific Sharing

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    Blog Post

    ...the Addgenies and congratulations to current and alumni Addgenies on our success. Thanks to our amazing...

13. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post

    ...confines channelrhodopsin activation to a small volume of brain tissue (~1 mm3). Current channelrhodopsins...

14. The time and cost required to make a plasmid

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    Blog Post

    ...labs are quite happy to share, labs that get high volumes of plasmid requests can end up refusing such requests...

15. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    ...easy detection with the appropriate fluorescent illumination in in vivo, ex vivo, or post-mortem experiments...

16. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    ...sequencing service will have different requirements for volume and concentration, and not all providers are equipped...

17. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    Blog Post

    ...the nervous system, including the motor cortex, cerebellum, and spinal cord. Cre-DOG is also suitable for...

18. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ...Noncoding RNAs. Annual Review of Biochemistry. 2012;81(Volume 81, 2012):145-166. doi:10.1146/annurev-biochem-...

19. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post

    ...tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional...

20. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    Blog Post

    ... Wu, K., Wu, R., Xiang, L., Fang, X., Jin, Z., Goldblum, D., Hasler, P.W., Scholl, H.P., Krol, J., & Roska...