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We narrowed to 474 results for: mag

Showing: 161 - 180 of 474 results

  1. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...looking for songs and sounds to synchronize with the images thereby making the videos more real. To give you...box, the Big Bang Theory set, and then use our imaginations to mold it all into a full laboratory. It is...It was a great treat for us to put our rusty imaginations to use in this world where we are often trained...

  3. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ... Pamela J. Hines PhD, Senior Editor at Science Magazine. Although we only walk one path at a time, the...spotted an opening for being an editor at Science magazine. That one I just could not resist applying for... Pamela J. Hines is a Senior Editor at Science magazine, published by the American Association for the...

  4. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...If you work in a lab that regularly does immunoimaging, there’s likely a large collection of antibodies...abstract protein during your sample prep process, imagining how it starts and if any steps could affect its...frustration when designing and troubleshooting immunoimaging assays. It can also elucidate information about...

  6. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...Once introduced, fluorescent proteins were best imaged after tissue clearing with SeeDB2 (Ke et al., 2016...tissue samples for High-resolution fluorescence imaging using SeeDB2." Bio-Protocol 8 (2018): e3046. Livet...PMC6245733. Susaki, Etsuo A., et al. "Whole-brain imaging with single-cell resolution using chemical cocktails...

  7. AAV Production in HEK293 Cells

    Type
    Protocol
    ...These cells were imaged with a 10X objective and were split 2 days before these images were taken. Last... Hazardous waste container pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents...Fisher, 12605010) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment... + 60 mL of 5 M Sodium Chloride + 4 mL of 1 M Magnesium Chloride Close the bottle and mix by inverting...

  8. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...which makes it possible to do things like in vivo imaging.   Excitation with NIR light also produces less...photosensitizers had similar rates of off-target damage to neighboring cells. TL;DR MG-2I-dL5** is a NIR...inactivation/cell ablation while having limited collateral damage to bystander cells. Are you ready to use MG-2I-...

  9. Hot Plasmids - January 2023

    Type
    Blog Post
    ...demonstrated the ability to be used as an endogenous RNA imaging tool.   Fig. 1: Csm, shRNA, and Cas13 ...ultrasound (FUS) to heat the tumor area, as the image below shows:    This system ablates tumors just...on the Anti-GFP [N86/8R] page for more details. Image credit: Addgene.   Find anti-GFP [N86/8R] here...

  10. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...Figure 1: Cas9 binding to the cleaved DNA duplex. Image from Richardson et al., 2016. We next wondered...donor DNA interaction with the Cas9-DNA duplex. Image from Richardson et al., 2016.   dCas9 enables... replacement frequencies that are an order of magnitude greater than transfection approaches. Moreover...

  11. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Two-Photon imaging was used to generate a 3D finemap of neurons in the mouse brain. MouseLight images can be...uniform and standardized way (Oh et al., 2014). Images yielded from this study can be found on the Allen... Lutton, E.M., Mu, D., Hudry, E., Hyman, B.T., Maguire, C.A. and Ramirez S.H. (2017). Trafficking of adenoassociated...

  12. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...phototoxicity Not susceptible Susceptible Subcellular imaging Improving Well-established High-throughput assays...properties. This approach can improve live-cell imaging by avoiding autofluorescence and phototoxicity ...Additional Resources on the Addgene blog Luminescent Imaging with Nano-lanterns  Technologies Enabled by NanoLuc...

  13. How Addgene Creates Content

    Type
    Blog Post
    ...correct. Final touches We try to use figures and images to help illustrate our points and break up walls...Addgene style.        Fig. 2: Reference image (left) provided to our graphic designer upon request...appropriately licensed and cited figures from papers or image sharing repositories. Then we format, do a final...

  14. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...types of breaks in tens of minutes – an order of magnitude faster than HDR. NHEJ is consequently the principal...this pathway and does not consider the missing or damaged nucleotides that are common to biological sources...DSB (blunt or near-blunt ends without nucleotide damage) such products are rare, probably accounting for...

  15. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...single domain antibody. To create their RANbodies, Yamagata et al used previously published nanobody sequences...RANbodies? Great! All of the RANbody plasmids from Yamagata et al can be found here. Can’t find a RANbody ...the comments below. References Sanes, J.R., & Yamagata, M. (2018). Reporter-nanobody fusions (RANbodies...

  16. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...of Gut microbiome by In situ Conjugation (MAGIC). With MAGIC, scientists introduce an Escherichia coli...genetic tools for microbiome studies.  Find the MAGIC plasmids here Paper-based synthetic biology approach...

  17. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...If you’re reading an article with images, now you have words, images, and links to pay attention to. If...If you’re watching a video, you now have moving images, noises and music, spoken words, and any onscreen...

  18. Cloning

    Type
    Guide
    ...gene of interest (YGOI) into a recipient plasmid. (Image from Plasmid 101: Restriction cloning ) Back to ...Gateway® Recombination Cloning Gateway cloning (Image from Plasmid 101: Gateway Cloning ) Gateway® cloning...gateway cloning. Back to Top TOPO® Cloning TOPO (Image from Plasmid 101: TOPO Cloning ) Toposiomerase based...chewed back by the 5' exonuclease. Gibson Assembly (Image from Plasmid 101: Gibson Assembly ) Back to Top ...your next cloning project. Golden Gate Cloning (Image from Plasmid 101: Golden Gate Cloning ) Back to ...
Showing: 161 - 180 of 474 results