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We narrowed to 568 results for: mme

Showing: 161 - 180 of 568 results

  1. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...with almost 80% rating it as very important. One comment reflected the desire for a flexible generic solution...Important collaborative functionalities include comments, notifications, sharing files, assigning team ...actions. These records can take the form of written comments, added files, notes etc. Detailed records about...

  2. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...corresponding fluorophore-conjugated antibody commercially available. In other cases, fluorophore-conjugated...this system is that streptavidin conjugates are commercially available for a wide range of fluorophores, ...on the location of the targeted markers. Some commercially available fix/perm kits allow for labeling of...

  3. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...IPI’s anti-integrin rAbs are unconjugated and recommended for use in flow cytometry. Based on the established...resulting in cells that elongated and morphed asymmetrically (Jo et al., 2022). By contrast, LVDP-binding...conferred less force, leading cells to spread into symmetrical circles. While elucidating the role of tension...

  4. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...DNA fragments. As a fun way to start, I highly recommend watching this entertaining video created by our...at 50 °C. After an hour or so, the sample is immediately ready to transform into competent cells. The ...contain the appropriate homologous sequences. NEB recommends an overlap of 15-40 bp, with a primer melting...

  5. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...addition of translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Snippert HJ, van der...Freundlich T, Weissman TA, Schoppik D, Wang XC, Zimmerman S, Ciruna B, Sanes JR, Luchtman JW, Schier AF....

  6. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...efficiently transduce your cells. In fact, we usually recommend testing more than one.  Most AAV serotypes have...the amount of virus you add, so it is strongly recommended to optimize to the “lowest number required” instead...reading! Good luck, and happy optimizing!  Recommended Reading Issa, S. S., Shaimardanova, A. A., Solovyeva...

  7. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...sections you’re particularly interested in, but I’d recommend watching the whole livestream for a more holistic...pre-publish) sound data even if it isn’t considered immediately impactful. Down the line we may see further adoption...issues in the biological sciences. I’d highly recommend listening to the panel discussion to learn a bit...

  8. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...template and CRISPR machinery, Quadros et al. recommend ribonucleoprotein (RNP) complexes of Cas9 protein...splice donor/acceptor sites, Miura et al. (2018) recommend targeting sites at least 100 bp away from the ...infundibulum. In vivo electroporation is performed immediately after injection. Image from Ohtsuka et al., 2018...

  9. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...mentorship relationships are valuable and highly recommended, a mentor with international experience provides... opportunities that truly exist outside their immediate frontiers. Moreover, sharing a culture/country...and advisor. For their own part, mentors receive immense personal gratification as they witness their student...

  10. Antibodies 101: Validation

    Type
    Blog Post
    ...the past few years to propose standards and recommendations for improving antibody use and reliability ... al., 2016). The major takeaway from these recommendations is: validate, validate, validate. This doesn.../19420862.2015.1100787  Taussig MJ, Fonseca C, Trimmer JS (2018) Antibody validation: A view from the ...

  11. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... to provide letters of reference, informal recommendations, more positive grant application and manuscript... active learning. Serve as a daily mentor for a summer undergrad, rotating grad student or high school...your perspective like intense exercise. I highly recommend running or biking or something that demands your...

  12. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...systems Tag Size (amino acids) Ligand Commercial ligands available Ease of use Tetracysteine...possibilities and save money). There aren’t as many commercial options for CLIP-tag and very few TMP-tag or ...Ohana, R. F., Urh, M., Simpson, D., Mendez, J., Zimmerman, K., Otto, P., Vidugiris, G., Zhu, J., Darzins...

  13. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...viral titer High vector genome (vg) doses are recommended for all systemic capsid applications. For example... elements, etc... *These are only starting recommendations; you will still need to experimentally determine...found a good fit (or if you need more options) we recommend you check out Caltech’s CLOVER Center’s extensive...support and input. Resources and references Recommended reading Challis R.C., Ravindra Kumar S., Chen...

  14. Deep Dive: qPCR

    Type
    Blog Post
    ... you probably know as TaqMan) and dsDNA dyes (commercial name: Sybr). However, there are other chemistries...here are some general helpful principles (and I recommend Bustin, 2017 for a more in-depth review.)  Your...normalization of signal. They are often included in commercial sets and most machines have a protocol that automatically...

  15. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ...antibody, especially a validated antibody, we recommend using antibody search engines and antibody data...search engine that you use? Let us know in the comments so we can add it to the table! And if you're interested...

  17. Editor's Choice, July 2016

    Type
    Blog Post
    ...put this technology into a concrete context. I recommend this post to anyone wondering if and how they ...Order-dependent Logic in vivo As always, feel free to comment on any of our posts if you have any questions or...

  18. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...pBACKBONE-XXXXX Tip: For a catalog of published and commercially available empty backbones, visit Vector Database...letter abbreviation of the wild type amino acid immediately followed by its position (number) relative to...

  19. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...career options in science, with Abigail Anderson commenting that “There is not just a scientist or a professor...Let us know about your educational events in the comments section below! Additional Resources Visit the...

  20. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...transform your plasmid DNA If you decided to use commercial competent cells, then you're best off following...of competent cells with those obtained using commercially available cells or previous batches of homemade...
Showing: 161 - 180 of 568 results