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We narrowed to 560 results for: mme

Showing: 161 - 180 of 560 results

  1. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...addition of translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Snippert HJ, van der...Freundlich T, Weissman TA, Schoppik D, Wang XC, Zimmerman S, Ciruna B, Sanes JR, Luchtman JW, Schier AF....

  2. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...efficiently transduce your cells. In fact, we usually recommend testing more than one.  Most AAV serotypes have...the amount of virus you add, so it is strongly recommended to optimize to the “lowest number required” instead...reading! Good luck, and happy optimizing!  Recommended Reading Issa, S. S., Shaimardanova, A. A., Solovyeva...

  3. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...template and CRISPR machinery, Quadros et al. recommend ribonucleoprotein (RNP) complexes of Cas9 protein...splice donor/acceptor sites, Miura et al. (2018) recommend targeting sites at least 100 bp away from the ...infundibulum. In vivo electroporation is performed immediately after injection. Image from Ohtsuka et al., 2018...

  4. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...sections you’re particularly interested in, but I’d recommend watching the whole livestream for a more holistic...pre-publish) sound data even if it isn’t considered immediately impactful. Down the line we may see further adoption...issues in the biological sciences. I’d highly recommend listening to the panel discussion to learn a bit...

  5. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...mentorship relationships are valuable and highly recommended, a mentor with international experience provides... opportunities that truly exist outside their immediate frontiers. Moreover, sharing a culture/country...and advisor. For their own part, mentors receive immense personal gratification as they witness their student...

  6. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...systems Tag Size (amino acids) Ligand Commercial ligands available Ease of use Tetracysteine...possibilities and save money). There aren’t as many commercial options for CLIP-tag and very few TMP-tag or ...Ohana, R. F., Urh, M., Simpson, D., Mendez, J., Zimmerman, K., Otto, P., Vidugiris, G., Zhu, J., Darzins...

  7. Antibodies 101: Validation

    Type
    Blog Post
    ...the past few years to propose standards and recommendations for improving antibody use and reliability ... al., 2016). The major takeaway from these recommendations is: validate, validate, validate. This doesn.../19420862.2015.1100787  Taussig MJ, Fonseca C, Trimmer JS (2018) Antibody validation: A view from the ...

  8. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... to provide letters of reference, informal recommendations, more positive grant application and manuscript... active learning. Serve as a daily mentor for a summer undergrad, rotating grad student or high school...your perspective like intense exercise. I highly recommend running or biking or something that demands your...

  9. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...viral titer High vector genome (vg) doses are recommended for all systemic capsid applications. For example... elements, etc... *These are only starting recommendations; you will still need to experimentally determine...found a good fit (or if you need more options) we recommend you check out Caltech’s CLOVER Center’s extensive...support and input. Resources and references Recommended reading Challis R.C., Ravindra Kumar S., Chen...

  10. Deep Dive: qPCR

    Type
    Blog Post
    ... you probably know as TaqMan) and dsDNA dyes (commercial name: Sybr). However, there are other chemistries...here are some general helpful principles (and I recommend Bustin, 2017 for a more in-depth review.)  Your...normalization of signal. They are often included in commercial sets and most machines have a protocol that automatically...

  11. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ...antibody, especially a validated antibody, we recommend using antibody search engines and antibody data...search engine that you use? Let us know in the comments so we can add it to the table! And if you're interested...

  13. Editor's Choice, July 2016

    Type
    Blog Post
    ...put this technology into a concrete context. I recommend this post to anyone wondering if and how they ...Order-dependent Logic in vivo As always, feel free to comment on any of our posts if you have any questions or...

  14. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...pBACKBONE-XXXXX Tip: For a catalog of published and commercially available empty backbones, visit Vector Database...letter abbreviation of the wild type amino acid immediately followed by its position (number) relative to...

  15. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...career options in science, with Abigail Anderson commenting that “There is not just a scientist or a professor...Let us know about your educational events in the comments section below! Additional Resources Visit the...

  16. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...website. 65 audience members gave up a beautiful summer Saturday to learn about science, but they didn’...efforts to engage the public - let us know in the comments section! More information about SITN: DayCon...

  17. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...transform your plasmid DNA If you decided to use commercial competent cells, then you're best off following...of competent cells with those obtained using commercially available cells or previous batches of homemade...

  18. Editor's Choice, September 2016

    Type
    Blog Post
    ...Historically we do better in September than in the summer months, but this is also our best month ever! Hats...to mention any apps you’ve found useful in the comments section to make this post even more useful to ...

  19. Editor's Choice, August 2016

    Type
    Blog Post
    ...Addgene Executive Director Joanne Kamens. I highly recommend taking a look at all of our posts as each one ... questions or suggestions for us, feel free to comment below. Happy reading! Tyler J. Ford ...

  20. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...Joanne's Reddit AMA Here they are annotated with my comments: Everyone on the team talks and listens in roughly...informal gatherings, like at lunch. Many experts recommend using anonymous surveys with regular frequency...
Showing: 161 - 180 of 560 results