We narrowed to 565 results for: mme

...DNA fragments. As a fun way to start, I highly recommend watching this entertaining video created by our...at 50 °C. After an hour or so, the sample is immediately ready to transform into competent cells. The ...contain the appropriate homologous sequences. NEB recommends an overlap of 15-40 bp, with a primer melting...

...addition of translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Snippert HJ, van der...Freundlich T, Weissman TA, Schoppik D, Wang XC, Zimmerman S, Ciruna B, Sanes JR, Luchtman JW, Schier AF....

...efficiently transduce your cells. In fact, we usually recommend testing more than one.  Most AAV serotypes have...the amount of virus you add, so it is strongly recommended to optimize to the “lowest number required” instead...reading! Good luck, and happy optimizing!  Recommended Reading Issa, S. S., Shaimardanova, A. A., Solovyeva...

...sections you’re particularly interested in, but I’d recommend watching the whole livestream for a more holistic...pre-publish) sound data even if it isn’t considered immediately impactful. Down the line we may see further adoption...issues in the biological sciences. I’d highly recommend listening to the panel discussion to learn a bit...

...template and CRISPR machinery, Quadros et al. recommend ribonucleoprotein (RNP) complexes of Cas9 protein...splice donor/acceptor sites, Miura et al. (2018) recommend targeting sites at least 100 bp away from the ...infundibulum. In vivo electroporation is performed immediately after injection. Image from Ohtsuka et al., 2018...

...mentorship relationships are valuable and highly recommended, a mentor with international experience provides... opportunities that truly exist outside their immediate frontiers. Moreover, sharing a culture/country...and advisor. For their own part, mentors receive immense personal gratification as they witness their student...

...the past few years to propose standards and recommendations for improving antibody use and reliability ... al., 2016). The major takeaway from these recommendations is: validate, validate, validate. This doesn.../19420862.2015.1100787  Taussig MJ, Fonseca C, Trimmer JS (2018) Antibody validation: A view from the ...

... to provide letters of reference, informal recommendations, more positive grant application and manuscript... active learning. Serve as a daily mentor for a summer undergrad, rotating grad student or high school...your perspective like intense exercise. I highly recommend running or biking or something that demands your...

...systems Tag Size (amino acids) Ligand Commercial ligands available Ease of use Tetracysteine...possibilities and save money). There aren’t as many commercial options for CLIP-tag and very few TMP-tag or ...Ohana, R. F., Urh, M., Simpson, D., Mendez, J., Zimmerman, K., Otto, P., Vidugiris, G., Zhu, J., Darzins...

...viral titer High vector genome (vg) doses are recommended for all systemic capsid applications. For example... elements, etc... *These are only starting recommendations; you will still need to experimentally determine...found a good fit (or if you need more options) we recommend you check out Caltech’s CLOVER Center’s extensive...support and input. Resources and references Recommended reading Challis R.C., Ravindra Kumar S., Chen...

... you probably know as TaqMan) and dsDNA dyes (commercial name: Sybr). However, there are other chemistries...here are some general helpful principles (and I recommend Bustin, 2017 for a more in-depth review.)  Your...normalization of signal. They are often included in commercial sets and most machines have a protocol that automatically...

...antibody, especially a validated antibody, we recommend using antibody search engines and antibody data...search engine that you use? Let us know in the comments so we can add it to the table! And if you're interested...

...plasmids on Addgene’s site (Side note: I always recommend you read the article to ensure you understand ...even easier and more useful, let us know in the comments. I hope this helps! Additional Resources on the...

...put this technology into a concrete context. I recommend this post to anyone wondering if and how they ...Order-dependent Logic in vivo As always, feel free to comment on any of our posts if you have any questions or...

...pBACKBONE-XXXXX Tip: For a catalog of published and commercially available empty backbones, visit Vector Database...letter abbreviation of the wild type amino acid immediately followed by its position (number) relative to...

...career options in science, with Abigail Anderson commenting that “There is not just a scientist or a professor...Let us know about your educational events in the comments section below! Additional Resources Visit the...

...transform your plasmid DNA If you decided to use commercial competent cells, then you're best off following...of competent cells with those obtained using commercially available cells or previous batches of homemade...

...website. 65 audience members gave up a beautiful summer Saturday to learn about science, but they didn’...efforts to engage the public - let us know in the comments section! More information about SITN: DayCon...

...Historically we do better in September than in the summer months, but this is also our best month ever! Hats...to mention any apps you’ve found useful in the comments section to make this post even more useful to ...

...Addgene Executive Director Joanne Kamens. I highly recommend taking a look at all of our posts as each one ... questions or suggestions for us, feel free to comment below. Happy reading! Tyler J. Ford ...