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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 397 results for: nam

Showing: 161 - 180 of 397 results

  1. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...Mike Lacy In order to study the conformational dynamics of the motor protein kinesin-1, the Twelvetrees...single-molecule FRET to study the intramolecular dynamics of the proteins in solution and when associated...

  3. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...protein timers FP timers have been used to: Monitor dynamics of vesicle fusion and release from the plasma ...overtime (Subach et al.) Monitor protein expression dynamics in a synthetic circuit (Saxena et al.) In a general...

  4. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...compensate between them. Another rule of thumb is if the names of the fluorescent markers are similar, you will...Chart.  Pro tip! Some antibodies go by different names depending on your flow cytometer’s setup. For instance...

  5. PhD Applications After COVID

    Type
    Blog Post
    ...program. Most applications will recommend that you name two professors in your application that you intend...reference your questions about the PI’s research or the name of the award you won for your conference presentation...

  7. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...recessive, severe, childhood-onset form of blindness name Leber’s Congenital Amaurosis (LCA). Shortly after...vectors to support trials for prevalent diseases - to name a few. CRISPR-based tools are also particularly...

  8. An Introduction to Adenovirus

    Type
    Blog Post
    ...Kafri et al., 1998). In fact, adenovirus gets its name because it was first isolated from human adenoid...targeted cells is less likely for adenoviral vectors – namely due to the removal of certain adenoviral genes ...

  9. Tips for arabidopsis transformation

    Type
    Blog Post
    ...from studying transcriptional and translational dynamics of genes and proteins in living plants, to complementing... plant selection markers (basta, hygromycin, kananamycin, tunicamycin) (Nakagawa et al., 2007). Alternatively...

  11. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...green portion of the visible spectrum (hence the name), which is due to a chromophore formed from a maturation...expressed in living cells, enabling the study of dynamic, physiological processes. Re-engineering GFP to...

  13. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...  Another area of maintenance is agreeing to the names of the things in our code. In some parts of our ...When this happens, we discuss and agree on a common name. While we do our best to write clean, reusable code...

  14. Bioinformatics at Addgene

    Type
    Blog Post
    ...we specified a select small number of input file names or filtered the input channel to only use the first...process printed one of the inputs, such as the file name. We started small with a print statement and tested...

  15. Hot Plasmids - November 2023

    Type
    Blog Post
    ....531939.  Qin, W., Cheah, J.S., et al. (2023). Dynamic mapping of proteome trafficking within and between...provides a unique new way to record and quantify the dynamics of promoter activity in single cells. Find iPAK4...

  17. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...track of which acronym is which. Acronym Full name Notes   Base transition Base change between purines...same protein (or its homologs) has more than one name. Indel Insertion/deletion A type of unwanted DNA...

  18. How to Write a Scientific Review Article

    Type
    Blog Post
    ...also recommend using the citation style of (Last name, Year) in the document while writing, because it...to whatever the journal requires. Using the (Last name, Year) format also has the benefit of exposing you...

  19. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...experiments crucial to studying neuronal circuits and dynamics. FPs are generally bright, stable and well tolerated...fluorescently-labeled antibodies. The “spaghetti monster” name is a reference to the deity of the social movement...

  20. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ... General protein expression  m15 pREP4*, ** Kanamycin (pREP4) Cis-repression of the E. coli T5 promoter...strains carry the DE3 lysogen, as indicated by the name of the strain. Conversely, other strains such as...
Showing: 161 - 180 of 397 results