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We narrowed to 399 results for: nans

Showing: 161 - 180 of 399 results
  1. Viral Production

    Type
    Collection
    ...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...
  2. Troubleshooting and Optimizing a Western Blot

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    Blog Post
    ...manufacturer. You can also consider using monoclonal or recombinant antibodies, which have much lower lot-to-lot ...point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase overall signal...enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein not properly...
  3. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ... their reagents: detection methods, synthetic nanobodies, SARS-CoV-2 protein expression, and more. We ...Quantification Methods to Consider by Jennifer Tsang The nanodrop vs. spec debate dominates DNA quantification discussions...
  4. Plant Plasmids and Resources

    Type
    Collection
    ...Fluorescence Complementation (rBiFC) and Förster Resonance Energy Transfer (FRET). Gateway Christopher Grefen...
  5. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...al. "The pCri system: a vector collection for recombinant protein expression and purification." PloS one..., Zachary T. Britton, and Anne S. Robinson. "Recombinant protein expression and purification: a comprehensive...
  6. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ... isolation, insertion and amplification of a recombinant DNA without sequence alteration. Molecular cloning...Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning strategies for protein expression." Current...
  7. CRISPR Guide

    Type
    Collection
    ...Cian, A., Itier, J. M., Anegon, I., Lopez, B., Giovannangeli, C., & Concordet, J. P. (2018). CtIP fusion ...prime editing systems by manipulating cellular determinants of editing outcomes. Cell . 184 (22), 5635-5652...
  8. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    Blog Post
    ...fragment, single-chain variable fragment (scFv), or a nanobody is one potential remedy for this problem within... Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...
  9. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions. Avidin and its derivatives...multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or bio-layer interferometry...
  10. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...how punctuation can be wildly funny as well as financially critical. Those who work on the publishing side...the right audiences and how open access affects financial models in information publication. Take a look...
  11. The Importance of High Titer for AAV Transductions

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    Blog Post
    ...References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration...
  12. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...F. R. Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies...trait: Absence of toxicity of R-body producing recombinant E. coli on paramecia. European Journal of Protistology...
  13. Antibodies 101: Isotypes

    Type
    Blog Post
    ...classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you heard of ..., but it’s also found in tissues. IgG is the predominant isotype during a secondary immune response, which...
Showing: 161 - 180 of 399 results