We narrowed to 388 results for: nans

1. Easing the Protein Purification Process with pCri

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   ...al. "The pCri system: a vector collection for recombinant protein expression and purification." PloS one..., Zachary T. Britton, and Anne S. Robinson. "Recombinant protein expression and purification: a comprehensive...

2. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

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   ...Croteau, J., Thakur, N., Jonigk, D., Davis, J. L., Hernandez, J. M., & Germain, R. N. (2022). IBEX: An iterative...Beuschel, R. T., Kabat, J., Croteau, J., Davis, J., Hernandez, J. M., & Germain, R. N. (2020). IBEX: A versatile...

3. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

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   ...reporters, such as Renilla luciferase (RLuc) or NanoLuc, require injection of substrate to produce bioluminescence...References 1 Bourdeau, R.W., Lee-Gosselin, A., Lakshmanan, A., Farhadi, A., Kumar, S.R., Nety, S.P., & ...

4. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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   ...LIC cloning vector and the pOKD plasmids. The recombinant Cry proteins will then be screened for toxicity...oxidase. The goal is to determine potential water contaminants and to subsequently develop technologies to ...

5. FastCloning: A Newer, Simpler Cloning Technique

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   ... isolation, insertion and amplification of a recombinant DNA without sequence alteration. Molecular cloning...Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning strategies for protein expression." Current...

6. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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   ...fragment, single-chain variable fragment (scFv), or a nanobody is one potential remedy for this problem within... Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...

7. In Vivo Biotinylation of Bacterial Fusion Proteins

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   ...a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions. Avidin and its derivatives...multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or bio-layer interferometry...

8. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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   ...One function that rose to the challenge was our Finance Team. With Addgene closed for almost two weeks ...for unknown amounts of time, we were facing a financial crisis. As a nonprofit organization, Addgene charges...

9. Science Careers: Unruly Interests Feed Many Paths

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   ...how punctuation can be wildly funny as well as financially critical. Those who work on the publishing side...the right audiences and how open access affects financial models in information publication. Take a look...

10. The Importance of High Titer for AAV Transductions

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    ...References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration...

11. Reagent Repositories Are Speeding up Science During the Pandemic

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    ...materials to be deposited into repositories, and financial support to maintain repositories’ capacity for...-5247(20)30004-5 McCray PB Jr, Pewe L, Wohlford-Lenane C, Hickey M, Manzel L, Shi L, Netland J, Jia HP...

12. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    ...your DNA prep and whether there are any other contaminants. Nucleic acids (DNA and RNA) absorb maximally... run, DNA fragments move through a micro- or nanofluidic channel and the sample is separated by electrophoresis...

13. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    ...They created an endogenous ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB...

14. R Bodies: Membrane-Rupturing Microscopic Tools

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    ...F. R. Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies...trait: Absence of toxicity of R-body producing recombinant E. coli on paramecia. European Journal of Protistology...

15. GCE4All: Making Genetic Code Expansion Accessible

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    ...their first protocol is available now. It is for recombinant expression of phosphoserine-including proteins...Site-specific Incorporation of Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https:/...

16. Antibodies 101: Isotypes

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    ...classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you heard of ..., but it’s also found in tissues. IgG is the predominant isotype during a secondary immune response, which...

17. Addgene’s Viral Service - Why Virus? Why Now?

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    ...project involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave the way for...export and shipping on dry ice. The Business and Finance Teams continue to facilitate the formation of partnerships...

18. Addgene: The First Twenty Years

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    ... we could use the same principles to provide recombinant antibodies, another plasmid-based product that... In March 2022, we launched our ready-to-use recombinant antibodies service, part of the Neuroscience ...

19. Antibodies 101: Fab Fragments

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    ...not a “Fab” fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide...conjugation.  Finally, Fvs are the most amenable to recombinant expression and will have the highest tissue penetration...

20. Antibodies 101: Secondary Antibodies

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    ...The introduction of antibody fragments, such as nanobodies, has introduced new options for increasing signal... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene  ...