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We narrowed to 404 results for: nans

Showing: 161 - 180 of 404 results
  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...expresses the λ Red recombinase enzymes, as well as a dominant-negative mutator allele of the E. coli MMR protein...and SnoopTag/SnoopCatcher The development of recombinant DNA technology has allowed molecular biologists...wine, fuels, pharmaceutical ingredients, and recombinant proteins. In order to keep production yields ...containing long homology arms and both auxotrophic and dominant selection markers, allowing for use with both ...
  2. Viral Production

    Type
    Collection
    ...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...
  3. Addgene Launches Partnership with IPI

    Type
    Blog Post
    ...collection of IPI’s well-validated and ready-to-use recombinant antibodies. The first collection, a set of integrin...sequences, in line with the rest of Addgene’s recombinant antibody collection, as well as access to IPI...
  4. Plant Plasmids and Resources

    Type
    Collection
    ...Fluorescence Complementation (rBiFC) and Förster Resonance Energy Transfer (FRET). Gateway Christopher Grefen...
  5. CRISPR Guide

    Type
    Collection
    ...Cian, A., Itier, J. M., Anegon, I., Lopez, B., Giovannangeli, C., & Concordet, J. P. (2018). CtIP fusion ...prime editing systems by manipulating cellular determinants of editing outcomes. Cell . 184 (22), 5635-5652...
  6. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...manufacturer. You can also consider using monoclonal or recombinant antibodies, which have much lower lot-to-lot ...point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase overall signal...enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein not properly...
  7. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ... their reagents: detection methods, synthetic nanobodies, SARS-CoV-2 protein expression, and more. We ...Quantification Methods to Consider by Jennifer Tsang The nanodrop vs. spec debate dominates DNA quantification discussions...
  8. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...al. "The pCri system: a vector collection for recombinant protein expression and purification." PloS one..., Zachary T. Britton, and Anne S. Robinson. "Recombinant protein expression and purification: a comprehensive...
  9. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ... isolation, insertion and amplification of a recombinant DNA without sequence alteration. Molecular cloning...Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning strategies for protein expression." Current...
  10. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...fragment, single-chain variable fragment (scFv), or a nanobody is one potential remedy for this problem within... Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...
  11. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions. Avidin and its derivatives...multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or bio-layer interferometry...
  12. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...how punctuation can be wildly funny as well as financially critical. Those who work on the publishing side...the right audiences and how open access affects financial models in information publication. Take a look...
  13. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...Phenol-chloroform extraction removes remaining contaminant proteins and RNase A from the DNA sample. When... This will help to remove proteins and other contaminants from the plasmid DNA. It is also advisable to...
  14. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...several micrograms of target DNA from just a few nanograms of template DNA through several cycles of denaturation...number of µl of dH 2 O equal to the number of nanomoles of DNA times 10. For example, if your lyophilized...
Showing: 161 - 180 of 404 results