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Showing: 161 - 180 of 381 results

  1. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions. Avidin and its derivatives...multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or bio-layer interferometry...

  2. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...how punctuation can be wildly funny as well as financially critical. Those who work on the publishing side...the right audiences and how open access affects financial models in information publication. Take a look...

  3. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...fragment, single-chain variable fragment (scFv), or a nanobody is one potential remedy for this problem within... Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...

  5. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration...

  9. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...F. R. Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies...trait: Absence of toxicity of R-body producing recombinant E. coli on paramecia. European Journal of Protistology...

  10. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...their first protocol is available now. It is for recombinant expression of phosphoserine-including proteins...Site-specific Incorporation of Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https:/...

  11. Antibodies 101: Isotypes

    Type
    Blog Post
    ...classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you heard of ..., but it’s also found in tissues. IgG is the predominant isotype during a secondary immune response, which...

  12. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post
    ...project involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave the way for...export and shipping on dry ice. The Business and Finance Teams continue to facilitate the formation of partnerships...

  13. Addgene: The First Twenty Years

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    Blog Post
    ... we could use the same principles to provide recombinant antibodies, another plasmid-based product that... In March 2022, we launched our ready-to-use recombinant antibodies service, part of the Neuroscience ...

  14. Antibodies 101: Fab Fragments

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    Blog Post
    ...not a “Fab” fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide...conjugation.  Finally, Fvs are the most amenable to recombinant expression and will have the highest tissue penetration...

  15. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...The introduction of antibody fragments, such as nanobodies, has introduced new options for increasing signal... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene  ...

  16. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ... Part 1 of this series). In any case, Cre is a dominant phenotype, while you’ll want the floxed gene to...836. PubMed PMID: 29768166. Zickler, Denise, and Nancy Kleckner. "Recombination, pairing, and synapsis ...

  18. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...surface and “receiver” cells express an anti-GFP nanobody on the cell surface. When the cells interact, ...fluorophore/antibody pairs. Because the anti-GFP nanobody cross-reacts with BFP, sender cells expressing...

  20. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...setting. Molecular diagnostics for the Zika virus predominantly look at viral load in patient blood and urine... carries out research in synthetic biology, nanomaterials science, and self-assembly for applications ...
Showing: 161 - 180 of 381 results