....   Overview of the restriction cloning process. Both the plasmid (blue, backbone) and the DNA ... beginning the restriction digest and ligation process, you should carefully choose your backbone and ...your experiments.     Alternatively, this whole process can be completed using a single enzyme if your ... more detail and verification required for the process to work well, so let’s go over the details step-by-step...

...workflow orchestration software for data pipeline processing (Nextflow), cloud infrastructure, and secure ...  In that post, we talked about Addgene’s data processing needs, our criteria for selecting a new workflow...single, organized pipeline and accelerated the processing of our sequencing data.”  Recently, Seqera published...

...division and large scale cell mobility are essential processes during embryonic development. These are required...differentiated body. In contrast, these dynamic processes are largely absent in adult organisms, where tissues... injury in the acutely inflamed glomerulus." Proceedings of the National Academy of Sciences (2016): 201606253...cell subsets in the skin-draining lymph node." Proceedings of the National Academy of Sciences 113.4 (2016...

...relaying key points about an unfamiliar topic or lab process. Along with our role as a plasmid repository, Addgene...-by-step instructions to navigate various lab processes.  Our most recent protocol video detailing Streaking...details every stage of the selection and checkout process on Addgene’s website: We’ll be continuously updating...

...development has caught up with it. The peer review process has been challenged with many closely spaced CRISPR... are trying to solve, and finally how we might proceed in the future. When we think of designing experiments...These functions help speed up the sgRNA design process by removing repetitive tasks and/or by providing...providing features to help with the post-design process such as primer and plasmid design. Examples:  “multiplex...

...Transformation grade DNA  Transformation is the process of inserting exogenous DNA in a microorganism. ...contaminants that have been used during the purification procedure. Good indicators of DNA purity are the absorbance...transfection grade DNA. Transfection is the process of inserting an external plasmid in mammalian cells...

... Addgene has no investors or stock. All of our proceeds go into running the repository and serving scientists...curious what specifically drives the decision making process at Addgene, just look to our core values. These...deposit and request plasmids (via web interface, processes, distribution, and customer service) Addgene provides...

...hike in. Navigating challenges: collecting and processing environmental samples Since the high temperatures...stabilization of unknown bacteria. Hiking all day and processing samples by night was fun but also very tiring...And it was exciting – one night while trying to process samples at the picnic table before dark, a bison...

... What is a transduction?  Transduction is the process of transferring foreign nucleic acid into a cell.... Transductions are similar to transfections, processes which introduce foreign nucleic acid into cells...   Figure 1: Transduction to expression process. A viral particle enters a host cell, enters the...

...Award highlights TTOs that accelerate science by processing agreements quickly! If your TTO receives a Golden...transfer professionals: Describe to your TTO how unprocessed agreements slow down your research; provide specific...all future requests/deposits. This reduces the processing time of request MTAs and deposit agreements to...

...background and/or connections to even begin this process. I am the cofounder and CEO of miLab, a Boston-...innovative ideas in all facets of the entrepreneurial process in order to develop those ideas into commercial...people for guidance.  Before even beginning the process of launching a company, you need to determine whether...

...Autophagy (Greek for “self-eating”) is a process by which cytoplasmic material, including organelles,...lysosomes for degradation. Autophagy is a dynamic process which involves autophagosome synthesis, delivery...inherent limitations because it turns a dynamic process into a static measurement and it provides limited...

...production process while still ordering viral vectors. Virus production can be a frustrating process that requires...developing ourselves as a knowledge base for the processes of viral production and testing so that researchers...

...fact, precise light-based control of biological processes is highly useful in other fields, including synthetic...to directly activate and inactivate biological processes. Chemical effectors are suboptimal for a number...intensity and wavelength can be used to study dynamic processes. Conversely, varied input patterns may also be...

...and effective. We do that through a standardized process of good research practice: computer simulations...’s experiments prove otherwise, the scientific process does seem to be working. I would argue that one...He and the CRISPR Babies is that the scientific process, as imperfect as it may be, does work when it is...

... is to quickly generate a single cell line and proceed to use it for experiments.” - Alex Chavez, researcher...scientists, producing lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5... genes involved in a wide variety of cellular processes. While these are widely requested and incredibly...

...overhangs based on regions of sequence homology. This process can occur through one of two pathways: RecA-mediated...-transformation incubation enhances the repair process, allowing SLIC to be used with very small amounts...practicalities of being SLIC To start the SLIC cloning process (see figure above), a fragment of interest is PCR...

...easier with more perspectives contributing to the process Senior people (of which there are never enough,...during the meeting.  Scientists do better with process, so don’t just get together and chat. Have an agenda...started. Have regular meetings. Experiment with the process. Have each person write a Development Plan. Set...

...effect of protein synthesis and degradation. These processes control the abundance of proteins in cells. Homeostatic...incorporation of puromycin into peptide chains." Proceedings of the National Academy of Sciences 51.4 (1964...puromycin of amino acid incorporation into protein." Proceedings of the National Academy of Sciences 45.12 (1959...

... at the mutation site, indicating that repair proceeded through NHEJ rather than HDR. In later characterization...characterization studies, the edited embryos proceeded to the blastocyst stage at similar rates to untreated...surrounding such research, it is crucial that scientists proceed with caution and with proper oversight from regulatory...