We narrowed to 601 results for: sam

...particles that are present in an AAV prep. AAV samples are first DNaseI digested to remove any residual...technology or a TaqMan primer/probe set can be used and samples are quantified by comparing to an AAV standard ...AAV, remember to account for the dilution of the sample for the DNAseI digest. Use good sterile technique.... 2. Choi, V.W., Asokan, A., Haberman, R.A., & Samulski, R.J. (2007). Production of recombinant adeno-...

...between endogenous and exogenous versions of the same target. In the 1980s, Munro and Pelham set out to... ideal.  Unexpected connections Right around the same time that Munro and Pelham were devising their tagging...from this year.  But are we all really using the same antibody developed almost 40 years ago? Well…kinda...distributing monoclonal antibodies derived from the same hybridoma developed by Evans, et al. In other cases...

...beam into a thin sheet to illuminate a fluorescent sample and captured the reflected light using a different...laser and the reflected light travel through the same objective lens. Using this method, these authors...microns, illuminates only one thin plane of the sample surrounding the focal plane of the detection lens...problem is to illuminate and acquire images of a sample from two opposing sides and merge the images, such...

...laboratory. All procedures are performed using the same three step protocol:  Fragment generation - Any...events, all linear fragments need to get into the same cell. For simple cloning requiring just one or two...vector and insert with separate primer pairs in the same PCR. Add homologous sequences to one of the primer...simply by adding all the necessary primers to the same PCR mix. Pairs of primers will amplify the whole...

...al., 2008) AAV6 (Towne et al., 2010), AAV8, -9 (Masamizu et al., 2011); AAV5 (Aschauer et al., 2013). Anterograde...PMCID: PMC5529245. Choi, V.W., McCarty, D.M., and Samulski, R.J. (2005). AAV Hybrid Serotypes: Improved Vectors...PMC2895694. Hollis, E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R. J., and Tuszynski, M. H. (2008). Efficient...PMID: 25374356. PubMed Central PMCID: PMC4269290. Masamizu, Y., Okada, T., Kawasaki, K., Ishibashi, H., Yuasa...PubMed Central PMCID: PMC5572074. Salegio, E.A., Samaranch, L., Kells, A.P., Mittermeyer, G., San Sebastian...

...our metabolic engineering case study of N-acetylglucosamine biosynthesis, we constructed a strain library...rounds of transformation to complete the N-acetylglucosamine strain library construction in 8 days. To ...numbers. (D) The effect of gene dosage on N-acetylglucosamine concentration. Image from the Yang lab. ... a lecturer in the College of Life Sciences that same year. Her research is focused on the escape mechanism...

... are represented as a series of dot plots in the same order. However, in an actual flow cytometry run,...parallel.  Let’s imagine we’re looking for cells in a sample that are negative for marker A and positive for... proxy for nucleic complexity of the cell.  The sample will have been labeled using a panel of markers... dots. In a contour plot, each line contains the same number of events. Thus, contour lines that are close...

...and subsequently leading to a positive signal. The same effect can be achieved for a fluorophore that relies...context of a paired fluorophore protein. Years later, Samie Jaffrey’s laboratory took advantage of their deep... Jaffrey lab asked:  Could an RNA aptamer do the same thing? Using the HBI derivative 3,5-difluoro-4-hydroxybenzylidene...· February 2008. 11. Svensen, Nina and Jaffrey, Samie R. “Fluorescent RNA Aptamers as a Tool to Study ...

...thanks to our guest blogger Samuel Mortensen from Northeastern University. Samuel Mortensen is a PhD candidate...This post was contributed by Samuel Mortensen, a PhD candidate at Northeastern University. Working with...19457242. PubMed Central PMCID: PMC2693113. Mortensen, Samuel, et al. "EASI transformation: An efficient transient...

...reintroduction, inversion and re-inversion happen at the same probability. Continuous inversion of the DNA sequence...recombination can only occur between target sites of the same sequence in FLEx vectors, the DNA sequence that ...neuron and its synapse should be visualized at the same time from two different plasmids this approach becomes...connectivity before and after the gene knockout in the same mouse.   Scientists always strive for more....

... purified from the system, researchers can run samples on SDS-PAGE gels containing Phos-Tag, a small molecule...non-phosphorylated serine at the TAG codon of the same expression vector used for phosphoserine incorporation...incorporation of serine) population of protein using the same expression plasmid. This is a particularly important...express the completely wild type protein from the same plasmid.  Upon verifying that serine phosphorylation...

...between samples. In addition to a DNA ladder standard, it is also a good idea to run an uncut sample of each...you to either stain your gel after you run your samples or add the stain as the gel is being made (post...containing ligation mixture with insert and backbone. Sample results indicative of successful and unsuccessful...

...seems obvious, it is important to remember that the same is true when designing gRNAs for using CRISPR technology...an alternative to HDR for introducing edits. The same locational constraints are even more exquisite for...concordance between different sequences targeting the same gene, suggesting that off-target effects did not...multiple gRNAs of different sequences give rise to the same phenotype in order to conclude that the phenotype...

...data. In some cases, CRISPR systems that are in the same class and type don’t share sequence similarity but...between two types, the Cas effector may have the same name but be completely different! They are merely...but it’s suspected that they don’t carry out the same types of adaptive immune functions that other CRISPR... 2000–2012. https://doi.org/10.1093/nar/gkz1197 Samai, P., Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-Aslan...

...campus speakers thoughtfully reminded us to use the same critical lens on our scientific community as we ... nor would you mention my small error and at the same time pass over in silence his very large one” (http...exoplanets into a successful PhD thesis. We use the same atmospheric physics to understand exoplanets that...happy to see so many others come out who feel the same.  I was also incredibly impressed with how well ...

...APC-Cy7) the brightness changes to "dim" for the same laser type. Knowing that the red laser excites at...fluorophore-conjugated antibodies together in the same color panel. In this case, make sure to compare ...overlap. Hence, they should not be used within the same panel.     Figure 4: Excitation and emission...virtually overlap, you cannot use them together in the same panel. Source: https://www.bdbiosciences.com/en-...

... However, the acceptor doesn’t need to go to the same exact location as the donor. As long as some of ...targeted nearby (such as a neighboring house on the same street as the donor), then FRET measurements are...Ebright, 2001). These Cy/NTA conjugates work the same way as NTA-sepharose beads used for protein purification... binding sites are adjacent to each other on the same protein and a negative control experiment conducted...

...the latest news and encourage your peers to do the same. Long-term lab conservation goals Lastly, I think...freezer to -70 °C and teach other labs to do the same. -70 °C is cold enough to keep some common types... about this, and even has a list of biological samples that are stored long term at -70 °C. My Green Lab...

...deserve, but, in this post, we'll provide a small sampling of the many amazing new plasmid tools that have... joined modules cannot be separated again by the same cloning enzymes. MXS (MluI-XhoI-SalI) chaining results...photo-label multiple mitochondrial populations within the same cell. Mitochondrial fusion is typically assayed ...different mitochondrial subpopulations within the same cell; by photoswitching PSmOrange and photoactivating... and more, to your protein of interest using the same vector system. The tRNA/aaRS pairs are delivered... experiments in several ways. The laboratory of Samie Jaffrey describes using these tags in an in-gel ...

...GUS staining or are not suited to studying large samples. RUBY-Red But recently, something new has been ...consisting of these three enzymes expressed under the same promoter with self-cleaving 2A site linkages and...transformation next to an untransformed seed from the same plant beginning to germinate. (Top right, second...