Hot Plasmids and Viral Preps - January 2021
Type
Blog Post
... to cut, Opto-Cre-Vvd is fast and can cut in as little as 2 hours.
Sheets, MB, et al., ACS Synth Biol...
We narrowed to 267 results for: ttl
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A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post... communicate your work, so you don’t need every little detail on the board. Poster layout While it’s ... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post...you are using. Dry nitrocellulose membranes are brittle, so proceed with caution. Unlike PVDF, they can... -
Engaging with science and society at pgEd
TypeBlog Post... and policy. In this post, I hope to tell you a little more about how I ended up at pgEd, what I do there... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...vector constructs. It consists of 2 plasmids: a shuttle vector (in which the transgene of interest is cloned... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...morgani and Phagocata gracillis, the process is a little more complicated. These strains are more picky ... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post... localizes primarily to the cell body with very little signal detected along the neurite (Figure 1.) Moreover... -
Lab Automation at Addgene
TypeBlog Post...putting some work into finding robots that’ll take a little labor off our hands. Good news! There is probably... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...both copies causes disease because too much or too little gene product disrupts normal function. Angelman... -
Hot Plasmids: Summer 2025
TypeBlog Post...and Jennifer Doudna’s labs engineered the tobacco rattle virus (TRV), a bipartite RNA virus, to deliver ... -
Common Injection Routes in Mice
TypeBlog Post...injections, intraperitoneal injections require a little bit of practice to ensure the proper injection ... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post... days. This means that viral production takes a little bit longer in insect cells. Processing Processing... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post... difficult part of a poster session, but with a little preparation and a few deep breaths the day-of, ... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...enriched sequences in the experiment and there was little enrichment of DNA targeted by a negative control... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post...sybodies, against membrane protein targets in as little as 3 weeks (Zimmermann et al., 2020). This selection... -
Academic vs. Industry Postdocs
TypeBlog Post...I’ve learned and hopefully make your decision a little easier if an industry postdoc is something that... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog Post...and low backgrounds – so much so that there was little range for detectable improvements in the methodology... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post...fragments can be pooled in one tube and ligated in as little as 30 minutes! Alternatively, you might consider... -
10 Basic tips for mammalian cell culture
TypeBlog Post...Finally, avoid sticking pipettes into solution bottles directly, rather decant the solutions into disposable... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...we love working with researchers on a model. The little knowledge that we have when we jump into a new ...