We narrowed to 268 results for: ttl

1. Adenoviral Vector Production and Troubleshooting

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   Blog Post

   ...vector constructs. It consists of 2 plasmids: a shuttle vector (in which the transgene of interest is cloned...

2. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   Blog Post

   ...morgani and Phagocata gracillis, the process is a little more complicated. These strains are more picky ...

3. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   ... localizes primarily to the cell body with very little signal detected along the neurite (Figure 1.) Moreover...

4. Lab Automation at Addgene

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   Blog Post

   ...putting some work into finding robots that’ll take a little labor off our hands. Good news! There is probably...

5. A Guide to Designing a Scientific Poster: Content, Layout, and More

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   Blog Post

   ... communicate your work, so you don’t need every little detail on the board.  Poster layout While it’s ...

6. Hot Plasmids and Viral Preps - January 2021

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   ... to cut, Opto-Cre-Vvd is fast and can cut in as little as 2 hours.  Sheets, MB, et al., ACS Synth Biol...

7. Making CRISPR Plasmids Using Fragmid

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   ... Plasmid pRDB_051 (for stable fly cell lines; attL and attR sequences for genome integration by phiC31...

8. Antibodies 101: Stripping and Reprobing Western Blots

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   Blog Post

   ...you are using. Dry nitrocellulose membranes are brittle, so proceed with caution. Unlike PVDF, they can...

9. Viral Vectors 101: Calcium Sensors

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   ...want to observe?’ This information, along with a little nuance discussed below, will help match your experiment...

10. Illuminating Epigenetics with A FRET Based Biosensor

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    ...both copies causes disease because too much or too little gene product disrupts normal function. Angelman...

11. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    ...enriched sequences in the experiment and there was little enrichment of DNA targeted by a negative control...

12. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ...sybodies, against membrane protein targets in as little as 3 weeks (Zimmermann et al., 2020). This selection...

13. Academic vs. Industry Postdocs

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    ...I’ve learned and hopefully make your decision a little easier if an industry postdoc is something that...

14. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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    Blog Post

    ...and low backgrounds – so much so that there was little range for detectable improvements in the methodology...

15. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post

    ...fragments can be pooled in one tube and ligated in as little as 30 minutes! Alternatively, you might consider...

16. 10 Basic tips for mammalian cell culture

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    ...Finally, avoid sticking pipettes into solution bottles directly, rather decant the solutions into disposable...

17. 3D Printing Meets CRISPR Cas9

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    Blog Post

    ...we love working with researchers on a model. The little knowledge that we have when we jump into a new ...

18. The Scientific Conference Poster Session: Tips for Success

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    Blog Post

    ... difficult part of a poster session, but with a little preparation and a few deep breaths the day-of, ...

19. Hot Plasmids: Summer 2025

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    Blog Post

    ...and Jennifer Doudna’s labs engineered the tobacco rattle virus (TRV), a bipartite RNA virus, to deliver ...

20. Common Injection Routes in Mice

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    Blog Post

    ...injections, intraperitoneal injections require a little bit of practice to ensure the proper injection ...