... Jorge Dubcovsky. Engineered biocontainable RNA virus vectors for non-transgenic genome editing across... improved vector system based on tobacco rattle virus (TRV) that simplifies gene silencing and genome ...

...transfer vectors and generating infectious virus. Protocol: Lentivirus Transfer Vector Production Protocol Lentiviral... or not you've efficiently generated infectious virus, plasmids expressing both the Cre and Flp recombinases... Viral Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of ...pAdenoX from Clontech and can be used to generate adenovirus expressing a variety of reporters as well as ...

...prevent substances in the blood, like proteins and viruses, from crossing into the brain. While helpful from...BioRender.com. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate...T.S., Miles T.F., Gradinaru V. Adeno-Associated Virus Toolkit to Target Diverse Brain Cells. Annual Review...T. F., & Gradinaru, V. (2022). Adeno-Associated Virus Toolkit to Target Diverse Brain Cells. Annual Review... C., Balaram, P., … Tasic, B. (2021). Enhancer viruses for combinatorial cell-subclass-specific labeling...Intravenous administration of the adeno-associated virus-PHP.B capsid fails to upregulate transduction efficiency...

...We began distributing ready-to-use virus preps through our viral service in late 2016 and requests are...

... time) got the idea to engineer bacteriophage (viruses that infect bacteria) for use as antibiotics. To...

...Transfection You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction...cell line is critical for obtaining high levels of virus. HEK293T cells should be split 3 times a week: Monday...

...of you who are not microbiologists, phages are viruses that infect bacteria and that can do so with exquisite...

...Biology Adapting Toehold Switches to Detect Zika Virus As always, if you have any questions or suggestions...

...! Delivering prime editors (PEs) via engineered virus-like particles (eVLPs) offers an option to transduce...Palczewski, K., & Liu, D. R. (2024). Engineered virus-like particles for transient delivery of prime editor...

...activation domain VP16 taken from the herpes simplex virus. tTA binds to promoters containing TetO elements... of VP16, the trans-activator of herpes simplex virus immediate early gene expression. Genes Dev. 1988...

...to find the right phage for the job. Phages are viruses that can infect and then kill bacteria they enter...

...on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

...up their own CRISPR arrays. On the other hand, viruses can evolve to evade CRISPR by mutating these sequences...

...of national boundaries. To be able to beat this virus, we will need to do the same with science,” said...groups have focused on iterating on methods for coronavirus research, sharing resources for COVID-19 research...our eye recently (not a comprehensive list). #coronavirus #SARSCoV2 #Covid19researchA thread ⬇️⬇️⬇️ — Addgene...

.... Originally isolated from the Mirabilis mosaic virus, the constitutively active M24 promoter has been...

...targeting strategy that allows adeno-associated virus (AAV)-borne payloads to be expressed in cells based...recombinase-expressing animal lines and retro-grade expressing viruses delivering additional recombinases 10–14 . Figure...principles for working with INTRSECT recombinases, viruses, and important controls to consider as part of ...

...on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

...cassette or an effector gene. HDVR (2X Hepatitis Delta Virus Ribozyme), a self-cleaving ribozyme. In the second-generation...

... genome (genome engineering) Produce synthetic viruses that can be used in research or for therapeutics...to antibiotics, degradative functions, and/or virulence. All natural plasmids contain an origin of replication...

...uses a biological sample to look for presence of a virus.” A scientist would likely mean “a test for the ...