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TypeBlog Post...regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal...
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Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...MiSeq and set up the run making sure to note the appropriate barcode for each sample. About 24 hours later... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...from Tang et al., 2020. G-baToN can also be applied to other fluorophore/antibody pairs. Because the... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: 21994751. PubMed... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post... also used to teach students about restriction mapping and western blotting. Students who participate ... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...influence (non-homologous end joining, or NHEJ), in approximately one percent of cases, the stem cell will seek... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...risk of dangerous DNA damage. If the way DNA is wrapped around histones changes, then gene expression can... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog Post...institutions are not local but are national issues. I applied and was successfully granted a travel award to ... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post... in biology but don’t really know how to start applying their skills to it. The Kittybiome project gives... -
Common Injection Routes in Mice
TypeBlog Post...expertise available in your lab or organization, and approvals from the organization overseeing your work. Good...