We narrowed to 1,010 results for: RING;

1. Antibodies 101: The Four ELISAs and When to Use Them

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   Blog Post

   ...typically use a competitive or inhibition ELISA when measuring small molecules that cannot bind efficiently to...

2. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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   Blog Post

   ...of mok results in translation of Mok and Hok, triggering cell death. However, the sok antisense-RNA binds...

3. Five Popular Model Organisms

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   Blog Post

   ...of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit flies are also...

4. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   Blog Post

   ... Mary Gearing...

5. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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   Blog Post

   ... Mary Gearing...

6. Plasmids 101: Restriction Cloning

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   Blog Post

   ...and plasmid backbone. Because you lose some DNA during the gel purification step, it is important to digest...

7. Adeno-associated virus (AAV) Guide

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   Guide

   ...on transgene expression and the expression of neighboring genes. rAAV episomes can develop chromatin-like...infect. Serotypes are determined by naturally occurring differences in the variable regions of the virus...vector is determined by the packaging plasmid used during production, as it carries the Cap region responsible...AAV method which has shown higher efficiency in delivering large genes makes use of split inteins, proteins...proteins that carry out a naturally occurring process known as protein trans-splicing. As these methods are... initial infection. The risk of spontaneously occurring replication-competent rAAV vectors is extremely...evolution to vector revolution: use of naturally occurring serotypes of adeno-associated virus (AAV) as novel...

8. Live and Let Dye: Self-Labeling Protein Tags

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   Blog Post

   ...function. If the idea of synthesizing your own ligands brings up bad memories of undergraduate organic chemistry...

9. Sequencing Options for CRISPR Genotyping

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   Blog Post

   ...validating edits.  Mismatch cleavage assays rely on pairing between the edited strand and wild-type strand ...

10. Optogenetics Guide

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    Guide

    ...of optogenetics integrates optics and genetic engineering to measure and manipulate cells (frequently neurons...Scientists have identified a variety of naturally occurring microbial opsins that respond to different wavelengths...proton efflux. Researchers have used genetic engineering to improve these natural opsins - by inducing...direct stimulation of ion channels. Naturally occurring channelrhodopsins were discovered in the green...to place the optic fiber outside of the brain, rendering the experimental procedure less invasive. Different...LARIAT Inhibits protein function by reversibly sequestering targets into large, optically assembled protein...Illumination causes the photoswitch to unfold, lowering the toxin's local concentration near the cell ...