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  1. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...adapted "self-cleaving" 2A peptides into their muliticistronic vectors. These peptides, first discovered in... of a 2A element, leading to separation between the end of the 2A sequence and the next peptide downstream...table below lists the four common 2A peptides employed by scientists. 2A cleavage is universal in eukaryotic...IRES and 2A peptides are not mutually exclusive elements. Labs have successfully utilized 2A and IRES ... these peptides are thought to function by making the ribosome skip the synthesis of a peptide bond at...cleavage with some of these peptides, no consensus has been reached on which peptide works best. Likely the...the specific genes cloned into the vector.3  2A Peptides To overcome some of the disadvantages of the ...

  2. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...gRNA. 2A-Puro In this plasmid, SpCas9 is fused to the puromycin resistance gene through a 2A peptide. The...transfection. The 2A "self cleaving" peptide causes the ribosome to translate Cas9-2A and the Puromycin...approrpriate cleavage site). Finally, in addition to the 2A peptide on its C-terminus, SpCas9 is also fused to a ...Puromycin resistance gene as separate peptides. PX459 This is the vector backbone. This particular backbone can...the most request plasmid in 2016 was... pSpCas9(BB)-2A-Puro (PX459) V2.0       Similar to last year, the...information). V2.0 This designation separates pSpCas9(BB)-2A-Puro (PX459) V2.0 from it's predecessor, which had... mutation has been corrected in V2.0. pSpCas9(BB)-2A-Puro (PX459) V2.0: What's not in the name? Not included...

  4. Hot Plasmids - August 2020

    Type
    Blog Post
    ... via BirA and AviTAG. pMAK contains the signal peptide of MBP, followed by the desired nanobody (NB) sequence...sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be exported to the periplasm of...localization signal or including fusions with Histone 2A or 2B can result in bright targeted expression while...

  5. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...short genetically encoded tetracysteine (Cys4) peptide tag could be used to label proteins in cells (Figure...covalent attachment of a fluorophore ligand to a peptide tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine...molecule fluorophores, such as rhodamines (Figure 2a), were discovered in the 19th century. Many of the...

  6. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... them with peptides that help mediate their transduction, such as the polyarginine peptide described in...iPSCs using a ~14.5kb minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2...

  7. Immunology Research Plasmids and Resources

    Type
    Collection
    ...natriuretic peptide precursor B BNP NPPC natriuretic peptide precursor C CNP NPR1 natriuretic peptide receptor...CCN3, IGFBP9 NPFF neuropeptide FF-amide peptide precursor FMRFAL NPPA natriuretic peptide precursor A ANF..., FGFB, HBGF-2 FPR1 formyl peptide receptor 1 FMLP, FPR FPR2 formyl peptide receptor 2 ALXR, FMLP-R-II...LMPH1A, PCL, VEGFR3 FPR1 formyl peptide receptor 1 FMLP, FPR FPR2 formyl peptide receptor 2 ALXR, FMLP-R-II...foveolin GLP1R glucagon-like peptide 1 receptor MGC138331 GLP2R glucagon-like peptide 2 receptor - GMFB glia...atrionatriuretic peptide receptor A) ANPRA, ANPa, GUC2A, GUCY2A, NPRA NPR3 natriuretic peptide receptor C/guanylate... HBGF8, HBNF, NEGF1 PYY peptide YY PYY1 QRFP pyroglutamylated RFamide peptide 26RFa, MGC119794, P518 RABEP1...

  8. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TTCCAGGCGGTGCGTAAGaagttcatcacagagcTGAAGGAACTGAGGCTGA fscn 2a TAL3266 & TAL3267 TAAATCACGAAAATCGATacctaactgcagaggcATTTGGTTTCAAGATCAA...TGCCAGCACAATAGATTGcaatggcaccgggttgGCAAGAGATCGCGTCGCA musashi-2a TAL3122 & TAL3123 TGGAAGCGGACGCGAGTCaagtcacctctggaagCCTGAACGATTCGCAACA...TCCAGGCAAGATTTGGGCtgatgctgatgatgacCGTGGCGATTGGGTCGTA Aminopeptidase-o TAL3020 & TAL3021 TGATCTGGATCCTAACAAggatgacttgcccctgtGTGCGAACACCAACCATA...TCTTCTTGTGCTCTTATTggcaggtacctggtgacTCACCTTATGGGCGCTGA matrixmetallopeptidase15 (membrane-inserted) TAL3484 & TAL3485 ...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ...dimerization , endosomal escape of cell-penetrating peptides , and more. nLuc/cLuc for split Firefly Luciferase...Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV9-CRISPR knockout...

  10. p53 Pathway

    Type
    Collection
    ...as PMAIP1 p14ARF Cyclin-dependent kinase inhibitor 2A p21 Cyclin-dependent kinase inhibitor 1A p48 Damage-specific...apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related cysteine peptidase CASP9 Caspase 9, apoptosis-related...Symbol Name 14-3-3-σ Stratifin Apaf-1 Apoptotic peptidase activating factor 1 ATM ATM serine/threonine kinase...apoptosis-related cysteine peptidase Cyclin B CCNB1 CCNB2 CCNB3 Cyclin B1, 2, or 3 Cyclin D CCND1 CCND2... PAG608 Zinc finger, matrin-type 3 PAI Serpin peptidase inhibitor, clade E (nexin, plasminogen activator...

  11. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...N133/21 scFv 2a RGS14 Rat Mouse 190530 Nav1.8 Na+ channel scFv [N134/12] N134/12 scFv 2a Nav1.8 Na+ channel... Mouse 190498 Homer1L scFv [L113/13] L113/13 scFv 2a Homer1L Mouse Mouse 190499 Homer1L scFv [L113/13]...Mouse 190500 Homer1L/S scFv [L113/130] L113/130 scFv 2a Homer1L/S Mouse Mouse 190501 Homer1L/S scFv [L113... Mouse 190502 Homer1L scFv [L113/27] L113/27 scFv 2a Homer1L Mouse Mouse 190503 Homer1L scFv [L113/27]...IgG2a 188173 Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/134R] NPY2R/Neuropeptide Y receptor type 2 Human...Parvalbumin Rat Mouse IgG2b 206639 Anti-Neuropeptide Y [L115/13R-2b] Neuropeptide Y Human Mouse IgG2b 206640 Anti-VGAT... 211594 Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/106R-2b] NPY2R/Neuropeptide Y receptor type 2 ...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pFBD-BirA_LRRK2:2141-2527 LRRK2 His, Biotin acceptor peptide tag polH Parkinson's Cheryl Arrowsmith 210986 pHTN-HaloTag_C9orf72...T7 Alzheimer's Andrew Deans 137187 pAAV FLEX eGFP 2A Tau WT WPRE MAPT CBA Parkinson's, FTD Matthew Nolan...Parkinson's Vikram Khurana 209083 pcDNA_FRT_SNCA-Avi_HygR-2A-BirA.dna(TO_deleted). (pEHA1448) SNCA Avi CMV Parkinson's...Khurana 209085 piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623) SNCA TRE Parkinson'...209086 piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628) SNCA Avi TRE Parkinson's...
Showing: 1 - 12 of 12 results