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We narrowed to 3 results for: Blasticidin-encoding lentiviral vector

Showing: 1 - 3 of 3 results

  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...lab. Plasmid Type Choose from: Encodes one insert, encodes gRNA/shRNA, or empty backbone. Purpose...biosensor affinity for calcium.”  Primary Vector Type The vector type should describe how your plasmid ...indicate type in “Vector Type Other” column. For example, you may enter “donor template vector”. Cloning...: mammalian expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, ...Resistance Enter bacterial antibiotic resistance encoded in the plasmid that should be used to maintain ... (prone to recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB...plasmids containing the ccdB gene, such as Gateway vectors, choose the ccdB Survival strain. For plasmids ...

  2. Lentiviral Prep Service

    Type
    Collection
    ..._Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled...Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral...Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled ...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...available in lentiviral format. Viral...

  3. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some...performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic overview of CRISPR...was the most efficient for gene editing. A virus encoding GFP plus a gRNA targeting GFP (pXPR_011, 59702...gift from Feng Zhang and is described in Improved vectors and genome-wide libraries for CRISPR screening....transduced (MOI = 37) and selected with 1 µg/mL blasticidin for 9 days. Single cells from a stable cell pool...then expanded for an additional 3 weeks under blasticidin selection. Anti-Cas9 Western blotting was performed...Lines with Lentivirus Isolating a Monoclonal Cell Population by Limiting Dilution Lentivirus Production...
Showing: 1 - 3 of 3 results