...fluorophore. Third, Brainbow-1 and Brainbow-2 each contained a “default” state; for example, Brainbow-1.0 expresses...visible with Brainbow-1 and -2. The general structure of Brainbow-1.0 is retained in Brainbow-3.0, but with...applying Brainbow to your research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids...attributes of Cre-lox to create the Brainbow mouse neural labeling system. Brainbow-1 and -2 have been further ...further refined to create Brainbow 3.2. Brainbow labeling has also been applied to cell marking and lineage ...systems, such as zebrafish and Drosophila. Why Brainbow? Brainbow highlights the trend in biology towards single-cell...synopsis of the differences amongst the Brainbow systems. Pre-Brainbow techniques, such as Golgi staining,...

...versions of Brainbow. Developed from Brainbow 3.0 and using the same fluorescent markers, Brainbow 3.1 and...neurons with dBrainbow and Flybow Based on the Brainbow 1.0 system, Drosophila Brainbow (dBrainbow; Hampel ...team appropriately named their technology Brainbow. Brainbow 1.0 used the fluorophores RFP (red), YFP ...membrane-tethered CFP (cyan). Brainbow 1.1 adds OFP (orange) (Livet et al., 2007). Brainbow uses a Cre/lox system...pairs. Brainbow 2.0 therefore acts as a switch between the default RFP and CFP, while Brainbow 2.1 switches...CFP and YFP. The most recent derivation of Brainbow, Brainbow 3.0 (Cai et al., 2013), incorporates the ...Pan et al., 2011), Brainbow was also used in zebrafish by injecting a CMV-Brainbow construct at the embryonic...

...reconstruction. To achieve stochastic multicolor labeling, Brainbow used the Cre-loxP system to express one of the... Cai et al., 2013). However, the brightness of Brainbow was inherently low. This is because the stochastic...the system Tetbow (Tetracycline trans-activator Brainbow) (Sakaguchi et al., 2018) (Figure 1). Once introduced...References Cai, Dawen, et al. "Improved tools for the Brainbow toolbox." Nature methods 10.6 (2013): 540. PubMed...Additional resources on the Addgene blog Read about Brainbow Learn about using AAVs for neuronal tracing Visit...

...Neuron. 2014. Feb 5;81(3):629-40.  Next-Gen Brainbow toolkit for neuronal imaging Joshua Sanes and ...Harvard University have developed a next-generation Brainbow toolkit for high-resolution fluorescent imaging...associated cell bodies. The first versions of the Brainbow system were reported in 2007. In order to extend...employs the Flp recombinase/FRT system in place of Brainbow’s Cre/loxP, allowing for simultaneous use of the... the FP sequence for separation from Cre-based Brainbow-expressing cells. Autobow plasmids are all-in-...Cre recombinase from the same transgene as the Brainbow cassette, simplifying experiments when additional...expression and increases the number of species in which Brainbow may be used. This system uses two plasmids in ...

...Controls Fluorescent Proteins Spagehetti Monster Brainbow Molecular Tools Tetracycline Transactivators Affinity...

...Green Red None Other Cre-dependent color switch Brainbow constructs Spaghetti monster tags Activity Cre-dependent...Ruby2sm-Flag (does not fluoresce) Cre dependent 1 Looger Brainbow Constructs 45185 AAV-EF1a-BbTagBY EF1a TagBFP ...

...Additionally, this system is less cumbersome than brainbow, another method for multi-color labeling of neurons...

...processes like decision making (Kim et al, 2017). “Brainbow” fish lines have been developed to enable high-resolution...

...Mouse Work on Exercise and Depression Read About Brainbow Resources on Addgene.org Find CRISPR Plasmids...

...proteins. Using one iteration of this system called Brainbow, the Ubow mouse strain has been created to fate...

...Article Description Expression System PI See article Brainbow constructs Cre-loxP recombination is used to create...