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We narrowed to 11 results for: Brainbow

Showing: 1 - 11 of 11 results

  1. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...fluorophore. Third, Brainbow-1 and Brainbow-2 each contained a “default” state; for example, Brainbow-1.0 expresses...visible with Brainbow-1 and -2. The general structure of Brainbow-1.0 is retained in Brainbow-3.0, but with...applying Brainbow to your research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids...attributes of Cre-lox to create the Brainbow mouse neural labeling system. Brainbow-1 and -2 have been further ...further refined to create Brainbow 3.2. Brainbow labeling has also been applied to cell marking and lineage ...systems, such as zebrafish and Drosophila. Why Brainbow? Brainbow highlights the trend in biology towards single-cell...synopsis of the differences amongst the Brainbow systems. Pre-Brainbow techniques, such as Golgi staining,...

  2. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...versions of Brainbow. Developed from Brainbow 3.0 and using the same fluorescent markers, Brainbow 3.1 and...neurons with dBrainbow and Flybow Based on the Brainbow 1.0 system, Drosophila Brainbow (dBrainbow; Hampel ...team appropriately named their technology Brainbow. Brainbow 1.0 used the fluorophores RFP (red), YFP ...membrane-tethered CFP (cyan). Brainbow 1.1 adds OFP (orange) (Livet et al., 2007). Brainbow uses a Cre/lox system...pairs. Brainbow 2.0 therefore acts as a switch between the default RFP and CFP, while Brainbow 2.1 switches...CFP and YFP. The most recent derivation of Brainbow, Brainbow 3.0 (Cai et al., 2013), incorporates the ...Pan et al., 2011), Brainbow was also used in zebrafish by injecting a CMV-Brainbow construct at the embryonic...

  3. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...reconstruction. To achieve stochastic multicolor labeling, Brainbow used the Cre-loxP system to express one of the... Cai et al., 2013). However, the brightness of Brainbow was inherently low. This is because the stochastic...the system Tetbow (Tetracycline trans-activator Brainbow) (Sakaguchi et al., 2018) (Figure 1). Once introduced...References Cai, Dawen, et al. "Improved tools for the Brainbow toolbox." Nature methods 10.6 (2013): 540. PubMed...Additional resources on the Addgene blog Read about Brainbow Learn about using AAVs for neuronal tracing Visit...

  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...Neuron. 2014. Feb 5;81(3):629-40.  Next-Gen Brainbow toolkit for neuronal imaging Joshua Sanes and ...Harvard University have developed a next-generation Brainbow toolkit for high-resolution fluorescent imaging...associated cell bodies. The first versions of the Brainbow system were reported in 2007. In order to extend...employs the Flp recombinase/FRT system in place of Brainbow’s Cre/loxP, allowing for simultaneous use of the... the FP sequence for separation from Cre-based Brainbow-expressing cells. Autobow plasmids are all-in-...Cre recombinase from the same transgene as the Brainbow cassette, simplifying experiments when additional...expression and increases the number of species in which Brainbow may be used. This system uses two plasmids in ...

  5. AAV Viral Preps

    Type
    Collection
    ...Controls Fluorescent Proteins, Spagehetti Monster, Brainbow Molecular Tools Tetracycline Transactivators, ...

  8. Control AAV Preps

    Type
    Collection
    ...Green Red None Other Cre-dependent color switch Brainbow constructs Spaghetti monster tags Activity Cre-dependent...

  11. Cre-lox system

    Type
    Collection
    ...Article Description Expression System PI See article Brainbow constructs Cre-loxP recombination is used to create...
Showing: 1 - 11 of 11 results