We narrowed to 73 results for: Cre recombinase

Learn how site specific recombinases like Cre-lox and FLP-FRT can be combined for complex control over gene expression in neuroscience experiments and more.

Overview of Cre-lox recombination and how it is used to make DNA modifications in transgenic animals, embryonic stem cells, and tissue-specific cell types.

Overview of recombinases that can be used as a Cre-alternative for site-specific recombination in Drosophila.

Learn about the top requested AAV from 2017: pmSyn1-EBFP-CRE, a modular tool for expressing Cre-recombinase in neurons.

Connie Cepko's lab at Harvard University has developed transcription factor and CRE recombinase variants activated by GFP. Learn more inside!

FLEx vectors utilize Cre/Lox to conditionally manipulate gene expression in vivo.

Researchers inactivate genes to investigate their function. This article describes techniques/plasmids used to knockout and knock-in specific genes in mammalian cells.

Learn about the sources of spontaneous recombination and optimizing Cre-recombinase dependent AAV vector expression in vivo.

Addgene adds new ready-to-use viral vectors to our service every month. Read all about the latest additions here!

Learn about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome or control gene expression, and find plasmids for your experiments.

Gamma-retroviral vectors and lentiviral vectors can be used in a wide variety of experiments including those involving genome engineering, RNAi, and inducible gene expression. In this post, learn about some of the most popular viral vectors available from Addgene and their uses.

Learn how you can use retrograde AAV for neuronal tracing.

Learn how FLEx and optogenetics combined can be combined to control gene expression with high spatial and temporal resolution.

Learn how the Gradinaru Lab at Caltech developed AAV vectors to target the peripheral and central nervous systems.

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

Addgene Board Member Connie Cepko gives us the scoop on progress in retinal gene therapies and the development of GFP-activatable tools in her lab.

Vectors containing the piggyBac transposon are now available at Addgene. Learn how these unique tools can be used for genome engineering!

Learn about the common techniques to creating engineered mouse models at embryonic stages and the pros and cons of different genome editing techniques.

Learn about key considerations when conducting optogenetics experiments in mice, including how to pick and delivery an opsin and stereotaxic surgery tips.

The Addgene blog has expanded tremendously in 2015. Check out our curated list of our top viewed posts from 2015!