Cre-ating New Methods for Site-specific Recombination in Drosophila
Type
Blog Post
... using multiple recombinases. To make multiple, precise genome edits, the recombinases used must have ...determine the cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested...however, the other recombinase/reporter pairs were inactive, even at very high recombinase levels. These data...toxicity of these recombinases by crossing flies homozygous for a UAS-driven recombinase with flies heterozygous...tubulin-GAL4. For Dre, Flp, KD, R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4...recombination in Drosophila, read on to learn about new recombinases suitable for this system.
Gerald Rubin’s lab...cross-reactivity, as well as low toxicity. FLP/FRT recombinases (from Saccharomyces cerevisiae) had been used...