FlipGFP, a novel fluorescence protease reporter to study apoptosis
Type
Blog Post
... self-assembling split GFP (GFP1-10 and GFP11) to increase fluorescence 10-fold after protease activity...modified a different self-assembling split GFP (GFP1-9 and GFP10-11) by flipping a single β-strand of GFP, ...β-strands 1-9 (β1-9) and the one ɑ-helix, (2) β-strand 10 (β10), and (3) β-strand 11 (β11). Each part contains...