We narrowed to 5 results for: Gak
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TypeCollection... WT YFP TARDBP YFP ALS Aaron Gitler 27695 GAK-pmCherryN1 GAK mCherry CMV Parkinson's Christien Merrifield...Spinocerebellar ataxia 18 Nicola Burgess-Brown 39042 GAK GAK TEV, His T7 Parkinson's Nicola Burgess-Brown 39529...Hallervorden-Spatz disease Cheryl Arrowsmith 79682 GAK_HUMAN_D0 GAK His, TEV Parkinson's John Chodera 79712 E2AK2...
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Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...photoactivatable system to cleave a target DNA sequence (Nihongaki, et al., 2015) resulting in a double strand break...might expect from the modular nature of Cas9, Nihongaki et al. showed that it was possible to swap out... Split Systems / Light-induced Dimerization Nihongaki et al., 2015 CRY2 and CIB1 from A. thaliana ...thaliana dCas9 Activation of gene transcription Nihongaki et al., 2015 paCas9 Magnet proteins from N. crassa...HDR, repression of gene expression by CRISPRi Nihongaki et al., 2017 Split-CPTS2.0 CRY2 and CIB1 from ...6289 . https://doi.org/10.1002/anie.201900788 Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto ...:963–966 . https://doi.org/10.1038/nmeth.4430 Nihongaki Y, Kawano F, Nakajima T, Sato M (2015) Photoactivatable... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...Addgene (Plasmid #83926). Live-Cell Voltage Sensor Ingaki and colleagues (8) were interested in using photoactivatable...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post... activates transcription of downstream genes. Nihongaki et al describe the targeted activation of endogenous...plasmids from this article through Addgene. Nihongaki et al., Chemistry and Biology. 2015 Feb 19; 22... -
Delivery Methods for Generating iPSCs
TypeBlog Post...Chen, Robert C. Robbins, Mark A. Kay, Michael T. Longaker, and Joseph C. Wu. "A nonviral minicircle vector...