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We narrowed to 13 results for: Gateway donor

Showing: 1 - 13 of 13 results

  1. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... the Gateway cloning method and its advantages. An introduction to Gateway technology The Gateway cloning...ccdB gene is excised from the donor vector.   Figure 2: The Gateway system adopts phage integration...one Gateway vector to produce the desired expression clone. This is possible thanks to the Gateway vectors...Systems (MuLE) Kit, the MultiSite Gateway cloning kit, and MultiSite Gateway plasmids. Constant reading frame...coli. Glossary of Gateway cloning vectors Vector type  Vector features Purpose Donor vector attP sites...links below to find Gateway plasmids for your organisms of interest: Find Gateway cloning plasmids here... needs. Since its invention in the late 1990s, Gateway cloning technology has become very popular as a...

  2. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ... the donor is quenched when FRET occurs. By photobleaching the acceptor, you release the donor's quenching...fluorescence decay time of the donor. When FRET occurs between the pairs, donor fluorescence is quenched and...non-radiatively from an excited molecular fluorophore (the donor) to another fluorophore (the acceptor) by means ...orientation will be fixed. The quantum yield of the donor (number of emitted photons per absorbed photons)...signal you should choose the highest quantum yield donor, the highest absorbing acceptor and fluorophores...used with Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest... the acceptor with the laser used to excite the donor. In that case the cross-talk is high and your background...

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...all the latest hot plasmid updates!     Tol2 Gateway-compatiable toolbox for studying the nervous system...system The Cole lab recently deposited the Tol2 Gateway-Compatible Toolobox, which will allow scientists...neurobiology and neuorodgenerative diseases. The Tol2 Gateway-Compatible Toolbox is based on the original Tol2kit...empty vectors that have standard cloning sites or gateway compatible cloning sites for easy cloning of your...toolbox adds new neuronal tools to the expanding gateway compatible vectors currently shared amongst the...2007. PubMed PMID: 17937395 Listen to Our Tol2 Gateway-Compatible Toolbox Podcast Segment The impact of...This makes mCyRFP1 a powerful tool for use as a donor in FRET (fluorescence resonance energy transfer)...

  4. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...requirements are different for the donor and acceptor fluorophores. The donor must be delivered to an exact... when donor and acceptor are close together. The binding of acceptors >100 Å away from the donor doesn...Let’s consider the donor first where labeling specificity is most important. Donor labeling and the need...for specificity Donor fluorophores need to go to the right place. Mis-targeted donors never “see” FRET...by a sea of uncoupled donors. The best method for targeting a fluorescent donor to your protein is to ...Madani et al., 2009). Donor and acceptors: Putting it all together The FRET donors and acceptors described...table below for donor and acceptor pairs that you can use in your FRET experiments. Donor Acceptor Tag...

  5. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...sex pilus, a “bridge” of cytoplasm that allows a donor cell to transfer genetic material to a recipient...also have more than one function: for example, Gateway cloning relies on genes found on an E. coli fertility...contain this F plasmid is critical for successful Gateway cloning. In addition, scientists have engineered...

  6. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...example, you may enter “donor template vector”. Cloning Method Choose from: Gateway cloning, gene synthesis...For plasmids containing the ccdB gene, such as Gateway vectors, choose the ccdB Survival strain. For plasmids...

  7. CRISPR References and Information

    Type
    Collection
    ...; dCas9 activator gateway donors: pAC84 , pAC1 , pAC147 , pAC148 , pAC149 ; Gateway destination: pAC90...algorithm to automate and optimize gRNA and HDR donor design. The current version supports SpCas9, SpCas9...HDR design; Deep sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED... Mammalian: FLAG tagging endogenous proteins pFETCh_Donor ; additional HDR & gRNA plasmids are available...

  8. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...on the Multisite Gateway® technology from Life Technologies. The MultiSite Gateway Kit is a customizable... systems. The MultiSite Gateway Kit contains a set of 12 promoter-less Gateway® entry clones to be used... phage integrase system which is the basis for Gateway cloning. The combination of all 11 att pairs in...customizable Gateway®-based toolkit that allows scientists to generate fusion proteins from any ORF available...as a standard entry clone. ORFs from an ORFeome Gateway library or any PCR-amplified ORF cloned into pDONR221...heterologous promoter outside of the Multisite Gateway® cassette, you can specifically express your new... combined in a specified order using Multisite Gateway cloning. A desired insert is cloned using traditional...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ...Insertion of 5' promoter/enhancer regions using Gateway cloning. Jorge Ferrer 16539 pBV-Luc Firefly Insertion...Bartel 101139 pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF) Firefly, Renilla Creating...® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2-ccdB-Nanoluc...® Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2...® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X...® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 106253 pLS-mP-Luc Firefly...split-luciferase complementation assay in plants using Gateway cloning. Use with 174051 Alberto Macho 174051 pGWB-cLUC...

  10. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...74334 pCR8-Magneto2.0-p2A-mCherry Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0...The collection is compatible with both standard Gateway cloning and the FNLCR Combinatorial Cloning Platform...gene are supplied to the reaction from a prebuilt donor plasmid library, available from Addgene. Site-specific...mammalian systems. The DULIP system is comprised of Gateway-compatible plasmids in which “bait” proteins are...available). Second, they co-transfected short-arm donor vectors containing mAID and either Neo or Hygro ...

  11. Molecular Cloning Techniques

    Type
    Guide
    ...completed via Gateway cloning. Addgene's collection contains thousands of Gateway donor , Gateway entry , and...backbone. Created with BioRender.com. Gateway Recombination Cloning Gateway cloning is a recombination-based...sequence. This fragment can then be cloned into a Gateway donor plasmid, which contains compatible attP sites...experimental goal. Although Gateway cloning is a simple and efficient cloning method, Gateway vectors and recombination...conditions. Read more in our Gateway Cloning blog post . Figure 2: Summary of Gateway cloning. Created with ...Molecular Cloning Techniques Restriction Enzyme Gateway TOPO Gibson Golden Gate & MoClo Ligation Independent...recombination-based cloning method. During Gateway, moving a piece of DNA from one plasmid into another is done via a single...

  12. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...assembly. Gersbach MuLE System Mammalian MultiSite Gateway recombination-based cloning of complex polycistronic...plasmids available for use in this Golden Gate and Gateway based assembly method: plasmids for assembly of...a single T-DNA binary vector of choice through Gateway recombination. Qi Multiplex Conditional Mutagenesis...pyogenes Blast Docampo pDonor_hU6 69312 Mammalian hU6 none S. pyogenes Ventura pDonor_mU6 69350 Mammalian mU6... 61285 Bacteria J23119 none S. pyogenes Beisel pZDonor-Nm-Cas9-gRNA-hU6 61366 Mammalian U6 none N. meningitidis... mU6 none S. pyogenes Ventura pDonor_sU6 69351 Mammalian sU6 none S. pyogenes Ventura pCRISPRyl 70007 ...

  13. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction...compatible with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your...yield of DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant...mechanisms. One of these methods uses splice site donor and acceptor sequences to reconstitute the two pieces...contains the 5' portion of the gene and a splice donor sequence at its 3' end, while the second vector ...
Showing: 1 - 13 of 13 results