A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
Type
Blog Post
...loxp site and one lox2272 site on either end of the transgene. Since loxp and lox2272 can not recombine...are comprised of different sequences (loxp and lox2272) and can not recombine with each other. When Cre...orientation for transcription. Upon recombination one of lox2272 sites will now be sandwiched between 2 loxp sites...binds to either pair of lox sites, let's say the lox2272, that are in the opposite orientation (direction...the loxp site is excised since it is between two lox2272 sites in the same orientation preventing the transgene...