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We narrowed to 30 results for: Lipi

Showing: 1 - 20 of 30 results

  1. Working with Nuclear Receptors

    Type
    Blog Post
    ... of fat-soluble hormones, vitamins, and dietary lipids. Included in this family are receptors for endocrine...oxysterols, bile acids, and numerous xenobiotic lipids derived from the diet. Additional members of this...Mangelsdorf, D. J. (2001). Nuclear receptors and lipid physiology: opening the X-files. Science 294, 1866...

  2. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...entry of a DNA template. Other techniques, such as lipid-mediated transfection (B in the figure above), are... Liu’s Lab has demonstrated the use of cationic lipid-mediated methods to deliver Cas9-gRNA RNPs to hair...PMC4938170. 2. Zuris, John A., et al. "Cationic lipid-mediated delivery of proteins enables efficient ...

  3. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...is because organic solvents can be quite harsh - lipids and soluble proteins can be lost during fixation... others. That said, organic solvents’ impact on lipids means that they are able to permeabilize your sample...examples of non-ionic detergents that interact with lipids and proteins in membranes and permeabilize non-...

  4. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...have a thinner peptidoglycan membrane and an outer lipid membrane. Common gram negative bacteria include ...negative bacteria, the alcohol destroys the outer lipid membrane, and the thin inner peptidoglycan membrane...

  7. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...other biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly visualized...1. Zacharias, David A., et al. "Partitioning of lipid-modified monomeric GFPs into membrane microdomains...

  8. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... sure to reveal even more insights. Super-duper lipid editor And if recruiting proteins to the cell membrane...phosphatidylcholine (PC), the predominant membrane lipid, to phosphatidic acid (PA) or it can swap phosphatidylchonine...

  9. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...measurement that is unaffected by intervening proteins, lipid bilayers, organelles or other cellular impediments... is required is that the proteins/nucleic acids/lipids of interest are fluorophore labeled and that these...

  11. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...express episomally long-term (AAV). If you perform a lipid-based or electrical-based transfection, most of ...

  14. CRISPR in the Clinic

    Type
    Blog Post
    ...delivery technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked to ...

  17. Hot Plasmids - November 2023

    Type
    Blog Post
    ...their webpage: mAb3D Atlas.    Figure 5: Delipidated mouse brain tissue was stained with Anti-PSD-...

  19. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...single step by denaturing proteins and dissolving lipids including those of the cell membrane. In some cases...

  20. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... Knockout Mouse Yusa 3rd 5 90,230 Mouse Lipid and Sphingolipid Metabolism Knockout Libraries 206248 206249...Library 117725 Knockout Human Vakoc 3rd 6 3,051 Human Lipid Droplet and Metabolism Library 191535 Knockout Human...
Showing: 1 - 20 of 30 results