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  1. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George G. Stokes) is ... shift is less than 50nm (often much less).  For LSS proteins, the Stokes shift is ≥ 100nm. Specifically...or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors: These FPs change...

  2. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...interest fused to the N-terminus of LSSmOrange pLSSmOrange-C1 Orange Mammalian Express a gene of interest... (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange Mammalian Express a gene of interest...interest fused to the C-terminus of LSSmOrange mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly...

  3. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression LSSmOrange 437 572 23 5.7 2.3 hr Monomer pLSSmOrange-C1 - Mammalian Expression pLSSmOrange-N1 - ...Expression LSSmKate1 463 624 3 3.2 1.7 hr Monomer pLSSmKate1-N1 - Mammalian Expression pLSSmKate1-C1 - Mammalian...pBAD/HisD-LSSmKate1 - Bacterial Expression LSSmKate2 460 605 4 2.7 2.5 hr Monomer pLSSmKate2-N1 - Mammalian...Mammalian Expression pBAD/HisD-LSSmKate2 - Bacterial Expression LSSmKate2-C1 - Mammalian Expression Jump...- Mammalian Expression pBAD-LSSmOrange - Bacterial Expression mKeima Red 440 620 3 6.5 4.4 hr Monomer ...

  5. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...interest fused to the N-terminus of LSSmOrange pLSSmOrange-C1 Orange Mammalian Express a gene of interest fused...Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange Mammalian Express a gene of interest... fused to the C-terminus of LSSmOrange mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly ...

  6. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...recognize codons found in highly expressed genes (Emilsson and Kurland, 1990). Controlling gene expression...15678157. PubMed Central PMCID: PMC1299251. 3. Emilsson, Valur, and Charles G. Kurland. "Growth rate dependence...

  7. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...with horseradish peroxidase (HRP; Kristensson and Olsson, 1971), plant lectins (wheat germ agglutinin WGA...Central PMCID: PMC4004172. Kristensson, K., and Olsson, Y. (1971). Retrograde axonal transport of protein...

  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...28288123 Listen to Our Podcast Segment on PhoCl LSSmCherry1 & RDSmCherry1: Engineering and directed evolution...influence of structure on an FP’s properties. pBAD-LSSmCherry1 is a long Stokes shift variant, which could be...

  10. Hot Plasmids - January 2023

    Type
    Blog Post
    ...next microscopy experiment, consider hfYFP and LSSmGFP.     Fig. 4: hfYFP retains folding and ...

  11. To Each HIS Own

    Type
    Blog Post
    ...Josephson S, Oestling M, Enfors SO, Persson I, Nilsson B and Uhlen M. Large-scale affinity purification...
Showing: 1 - 16 of 16 results