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We narrowed to 16 results for: Lss

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Showing: 1 - 16 of 16 results

  1. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George G. Stokes) is ... shift is less than 50nm (often much less).  For LSS proteins, the Stokes shift is ≥ 100nm. Specifically...or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors: These FPs change...

  2. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Expression LSSmCherry1 450 610 10 6.2 Monomer pBAD-LSSmCherry1 - Bacterial Expression LSS-mKate1 463 ...Expression LSSmGFP 400 510 18 4.7 Monomer (A206K) pcDNA3.1-LSSmGFP - Mammalian Expression pBAD-LSSmGFP - Bacterial...Expression LSSmOrange 437 572 23 5.7 2.3 h Monomer pLSSmOrange-C1 - Mammalian Expression pLSSmOrange-N1 - Mammalian...Monomer pLSSmKate1-N1 - Mammalian Expression pLSSmKate1-C1 - Mammalian Expression pBAD/HisD-LSSmKate1 - Bacterial...Bacterial Expression LSSmKate2 460 605 4.4 2.7 2.5 h Monomer pLSSmKate2-N1 - Mammalian Expression pBAD...pBAD/HisD-LSSmKate2 - Bacterial Expression LSSmKate2-C1 - Mammalian Expression Return to top Photoactivatable... Mammalian Expression pBAD-LSSmOrange - Bacterial Expression mKeima (pH sensitive) 440 620 3.5 6.5 3 h...

  3. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...interest fused to the N-terminus of LSSmOrange pLSSmOrange-C1 Orange Mammalian Express a gene of interest... (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange Mammalian Express a gene of interest...interest fused to the C-terminus of LSSmOrange mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly...

  5. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...recognize codons found in highly expressed genes (Emilsson and Kurland, 1990). Controlling gene expression...15678157. PubMed Central PMCID: PMC1299251. 3. Emilsson, Valur, and Charles G. Kurland. "Growth rate dependence...

  6. Fluorescent Proteins: FRET

    Type
    Collection
    ...pBAD , pLSSmOrange-mKate2 LSSmOrange* mScarlet3 437 0.45 592 104,000 0.75 5.8 4.3 pLSSmOrange-N1 , pmScarlet3...-N1 , pmiRFP720-N1 LSSmOrange* mKate2 437 0.45 633 62,500 0.40 5.6 3.7 pLSSmOrange-N1 , mKate1.31-pBAD...

  7. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...28288123 Listen to Our Podcast Segment on PhoCl LSSmCherry1 & RDSmCherry1: Engineering and directed evolution...influence of structure on an FP’s properties. pBAD-LSSmCherry1 is a long Stokes shift variant, which could be...

  8. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...with horseradish peroxidase (HRP; Kristensson and Olsson, 1971), plant lectins (wheat germ agglutinin WGA...Central PMCID: PMC4004172. Kristensson, K., and Olsson, Y. (1971). Retrograde axonal transport of protein...

  10. Hot Plasmids - January 2023

    Type
    Blog Post
    ...next microscopy experiment, consider hfYFP and LSSmGFP.     Fig. 4: hfYFP retains folding and ...

  11. To Each HIS Own

    Type
    Blog Post
    ...Josephson S, Oestling M, Enfors SO, Persson I, Nilsson B and Uhlen M. Large-scale affinity purification...

  16. Luciferase Plasmid Collection

    Type
    Collection
    ...acceptor. LumiFluors: EGFP-NanoLuc ( GpNLuc ) and LSSmOrange-NanoLuc ( OgNLuc ) fusions for BRET-based imaging...
Showing: 1 - 16 of 16 results