...1.80-2.00). Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop, if two...

...cover the general procedure and features to keep in mind when using any centrifuge. However, each centrifuge...

...amount of enzyme for your reaction. However, keep in mind that restriction enzyme activity is determined under...

...pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments require electroporation...

...it will take time so be patient and keep an open mind. First let’s clear up a misconception about being...in your team: learn what motivates them (keep in mind that it can be very different from what motivates... how to face a particular situation. But keep in mind that a good manager should help you work through...you and you need to encourage them to speak their minds. They will make mistakes and you will take responsibility...

...introduction to fluorescent biosensors, but keep in mind that there is a lot of variety in how biosensors...are commonly used for.  Table 1. Points to Keep in Mind When Performing Biosensor Experiments Experiment... blue flame extracellular glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about...

...development is not one of the things that comes to mind. The truth is, there is a lot of software development...short periods of time called sprints. The team is mindful of the number of stories we commit to for each ...reviews because it’s like a little peek into the mind of the developer and I get to see an approach that...

...priorities to attend to. Being mindful looks like: “When you have time, would you mind helping me with….” vs. ... be done by the researchers themselves.  Be mindful that your mentor will still have their own deadlines...

...what makes a good manager? First, it takes an open mind to be willing to learn and grow in skills. Managing...miserable.   A good manager keeps these factors in mind at all times when making decisions, assignments ...

...meeting people with different mindsets and approaches can truly broaden your mind. When I first began collaborating...the perspective of my potential collaborators in mind. Being at a university allows you to approach people...and organizing collaborations, keep these jobs in mind! References 1. Sinche, Melanie, et al. "An evidence-based...tools like alumni networks. I find it useful to remind myself to check in regularly with collaborators...in regularly, and maintaining useful notes that remind me about the current state of a partnership. Every...

...great activity for both exercise and clearing your mind. And because the outdoors is so huge, these are ... help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace practice...

...toxic in high amounts? It is always good to keep in mind that plasmids with low to medium copy numbers can... considerations are especially useful to keep in mind if you are planning to purify your plasmid DNA: ...

...outcomes clear? If you don’t have clear outcomes in mind, you won’t be able to communicate these well to ...articulate the desired outcomes. Begin with the end in mind. Specify desired results. Are you delegating to...

...irreproducibility. Luckily, there were other academics and like-minded organizations with similar ideas who were also ...impactful opportunities - There are many brilliant minds out there, but how do you get them to connect? Through...

... superb and detailed portrayal of yourself. Be mindful that January and February represent peak season...program of your dream.  “Chance favors the prepared mind” by Louis Pasteur These tips are a set of common...        5. Application submission  Set a calendar reminder for every deadline and shoot to submit your application...

...plasmids were not designed with gene activation in mind. “We had not managed to create a system to allow...lentiviral CRISPR libraries and keep the following in mind: These libraries are pooled. Most of these screens...

...into a startup company. However, like many like-minded people, I quickly realize that a company requires...should initially include a visionary, a science-minded individual, and a specialist. Notably, the team...

...middle of my presentation, lost in a sea of data, my mind went completely blank. After several seconds of ... classmates so they could understand it. When my mind went blank, my options were to stop talking until...

...next week!”, and “This is his job!” filled my naive mind. Moving forward from that first experience, I learned...process, she has faith in you! Additionally, keep in mind that universities require application submission...the mission of the agency and benefits the field reminds the reviewer why your project is the project to...

...bit more challenging. With these considerations in mind, when planning an experiment users should weigh ...collected from an unconcentrated prep. With this in mind, if the experiment requires a certain volume of ...