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We narrowed to 38 results for: PHP

Showing: 1 - 20 of 38 results

  1. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    ...transfer to the rat CNS: AAV PHP. EB vector dose–response and comparison to AAV PHP. B." Gene therapy 25.5 ...receptor for AAV-PHP. B capsids." bioRxiv (2019): 538421. If you’re interested in how PHP.B performed in ..., et al. "The GPI-Linked Protein LY6A Drives AAV-PHP. B Transport across the Blood-Brain Barrier." Molecular...Juliette, et al. "The neurotropic properties of AAV-PHP. B are limited to C57BL/6J mice." Molecular Therapy...Intravenous administration of the adeno-associated virus-PHP. B capsid fails to upregulate transduction efficiency...complex (LY6A) as a receptor for PHP.B. Read on to learn how the design of PHP.B may have contributed to this...receptor for PHP.B, and how the Deverman and Wilson labs determined LY6A was a receptor used by PHP.B viruses...

  2. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...at Caltech stepped up to the challenge. Find the PHP Plasmids Here! Their Solution: Use the CREATE method...of AAV-PHP.B versus AAV-PHP.eB in the CNS. A GFP transgene was packaged into either an AAV-PHP.B or an ...engineer two new IV injectable AAV capsids, AAV.PHP.eB and AAV.PHP.S, that target the CNS or the PNS, respectively...from. Additionally, AAV.PHP.eB was used at a lower dose than it’s parent AAV.PHP.B: 1x1011 vs 1x1012 viral.... Figure 3 shows a gross comparison of AAV.PHP.eB and AAV.PHP.S to their parental vectors. To quantify... of neurons were measured. On average, AAV.PHP.eB and AAV.PHP.S had 1.5- to 2-fold higher GFP intensity...quantified transduction efficiency of AAV-PHP.eB and AAV-PHP.S versus their parental AAV vectors. What...

  4. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...capsid in the Addgene repository. Like its parent AAV-PHP-B, it can be produced with high viral yields relative..., 2019 AAV-PHP.B2 CNS Mice Yes (LY6A) Deverman et al., 2016 AAV-PHP.B3 CNS Mice ... AAV-PHP.eC CNS: neurons and astrocytes Mice No (LY6C1) Shay et al., 2023 AAV-PHP.V1* CNS...2020 AAV-PHP.N CNS: neurons Mice Yes (LY6A) Kumar et al., 2020 AAV-PHP.B4 CNS Mice...the more efficient AAV-PHP.eB, developed and deposited by the same lab. AAV-PHP.eB is the most commonly ... lower viral titers than AAV-PHP.B (e.g., 1 x 1011 vg/mouse), AAV-PHP.eB can transduce a greater number...AAV-PHP.eB (Fig. 3d and 4d, Jang et al., 2023). When combined with a specific promoter, AAV-PHP.AX shows...

  5. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ... AAV-PHPeB Optogenetics Jonathan Ting New viral prep pAAV_BiSSTe10_dTomato_nlsdTomato AAV-PHPeB Control...AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control... AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_BiVIPe4_dTomato_nlsdTomato AAV-PHPeB Control...dTomato_nlsdTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_BiLAMP5e3_ChR2_mCherry AAV-PHPeB Optogenetics...dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_BiPVe4_ChR2_mCherry AAV-PHPeB Optogenetics...dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_BiPVe3_ChR2_mCherry AAV-PHPeB Optogenetics...dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_BiSSTe4_ChR2_mCherry AAV-PHPeB Optogenetics...

  6. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...packaged in AAV9-X1.1!  We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab at the Allen...Engel New serotype pAAV-hSyn-Cre-P2A-dTomato AAV PHP.eB Recombinases Rylan Larsen New serotype AiP13778...pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) AAV PHP.eB Recombinases Jonathan Ting New viral prep AiP13779...3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) AAV PHP.eB Recombinases Jonathan Ting New viral prep AiP13781...pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) AAV PHP.eB Recombinases Jonathan Ting New viral prep pAAV-CAG-GFP...Edward Boyden New serotype pAAV-hSyn-EGFP AAV PHP.eB Controls Bryan Roth New serotype AiP13044 - pAAV-AiE0779m...3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) AAV PHP.eB Controls Jonathan Ting New viral prep AiP13038...

  7. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...brain, would you recommend using the PHP.S capsid over the PHP.eB (the capsid we have been using)?Would...transduction in microglia? I have been using the AAV.PHP.eb for neuron, oligodendrocyte, and astrocyte specificity...Assisted Spectral Tracing (VAST) experiments using PHP.eB in the brain give some insight into codelivery ...blood brain barrier we would currently recommend PHP.V1 or AAV-BR1. We recommend you keep an eye out for...area.   Q8: On dense labeling in the brain with PHP.eB: A recent CLOVER collaboration injected 1e+13 vg...phenotypes (that’s almost 100ul/animal for the PHP.eBs that we have in house!), which seems to me a very... repository, this application is an outlier for PHP.eB (1x1011 - 1x1012 vg/mouse is more standard) and...

  8. New Viral Vectors - March 2024

    Type
    Blog Post
    ...pAAV.CMV.PI.EGFP.WPRE.bGH  AAV PHP.eB Controls Wilson New viral vector pENN.AAV.EF1a.eGFP.WPRE.rBG AAV PHP.eB Controls Wilson...pAAV-mDlx-NLS-mRuby2  AAV PHP.eB Controls Gradinaru New serotype pAAV-CaMKIIa-mCherry  AAV PHP.eB Controls Deisseroth...well as seven vectors newly available in the AAV PHP.eB serotype. We've also added two viral vectors that...New serotype pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE AAV PHP.eB Biosensor Kim New serotype AAV-mDlx-jGCaMP8s-... Wilson New serotype pAAV-CaMKIIa-EGFP AAV PHP.eB Controls Roth New serotype pAAV-hDlx-Flex-dTomato-Fishell...Deisseroth New serotype pAAV-Ef1a-mCherry  AAV PHP.eB Controls Deisseroth New serotype Looking for ...

  9. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...experiments delivered ChRgers by intravenous injection of PHP.eB, an AAV capsid capable of broad transduction of...need for intracranial injections. When packaged in PHP.eB, ChRgers and ChR2(H134R) both reach the brain, ...make it better suited for systemic delivery with PHP.eB compared to the existing ChR2(H134R).  In the second...higher-photocurrent properties when paired with PHP.eB’s systemic delivery enables minimally-invasive gene...resources on the Addgene blog Read more about the PHP.eB AAV capsid Read more about optogenetics Learn about...

  10. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...: pAAV-AiE0452h_3xC2-minBG-jRGECO1a-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science... pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science...: pAAV-AiE0452h_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science...

  11. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...Patriarchi New viral service pGP-AAV-syn-jGCaMP8m-WPRE PHP.eB Biosensors GENIE Project New serotype pAAV-hSyn-GRAB_OT1.0...serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls Feng New viral service, multiple serotypes...

  12. Hot Plasmids February 2024

    Type
    Blog Post
    ...using AAV1-X1 (Chen et al., 2023); then, when AAV-PHP.eB was injected three weeks later, that LY6A transgene...transgene rescued the enhanced brain potency of AAV-PHP.eB.    Figure 4: AAV9-X1 and AAV9-X1.1 efficiently...

  13. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=39322032   No terminator is 100% efficient...CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=17554393   Use of mammalian poly(A) ...

  15. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... al., 2020) derived from bacterial phytochrome (BphPs) photoreceptors have higher apparent cellular brightness...Brightness in HeLa cells vs. iRFP713, % d Ref BphP-based FPs miRFP670 https://www.addgene.org/79987...

  18. Hot Plasmids - May 2022

    Type
    Blog Post
    ...left) and transduction patterns (right).  Both AAV-PHP.eB and the AAV.CAP-B capsids are derived from AAV9...
Showing: 1 - 20 of 38 results