We narrowed to 40 results for: PHP

...transfer to the rat CNS: AAV PHP. EB vector dose–response and comparison to AAV PHP. B." Gene therapy 25.5 ...receptor for AAV-PHP. B capsids." bioRxiv (2019): 538421. If you’re interested in how PHP.B performed in ..., et al. "The GPI-Linked Protein LY6A Drives AAV-PHP. B Transport across the Blood-Brain Barrier." Molecular...Juliette, et al. "The neurotropic properties of AAV-PHP. B are limited to C57BL/6J mice." Molecular Therapy...Intravenous administration of the adeno-associated virus-PHP. B capsid fails to upregulate transduction efficiency...complex (LY6A) as a receptor for PHP.B. Read on to learn how the design of PHP.B may have contributed to this...receptor for PHP.B, and how the Deverman and Wilson labs determined LY6A was a receptor used by PHP.B viruses...

...at Caltech stepped up to the challenge. Find the PHP Plasmids Here! Their Solution: Use the CREATE method...of AAV-PHP.B versus AAV-PHP.eB in the CNS. A GFP transgene was packaged into either an AAV-PHP.B or an ...engineer two new IV injectable AAV capsids, AAV.PHP.eB and AAV.PHP.S, that target the CNS or the PNS, respectively...from. Additionally, AAV.PHP.eB was used at a lower dose than it’s parent AAV.PHP.B: 1x1011 vs 1x1012 viral.... Figure 3 shows a gross comparison of AAV.PHP.eB and AAV.PHP.S to their parental vectors. To quantify... of neurons were measured. On average, AAV.PHP.eB and AAV.PHP.S had 1.5- to 2-fold higher GFP intensity...quantified transduction efficiency of AAV-PHP.eB and AAV-PHP.S versus their parental AAV vectors. What...

...also like: Viral Service: All AAV Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part of our ...

...capsid in the Addgene repository. Like its parent AAV-PHP-B, it can be produced with high viral yields relative..., 2019 AAV-PHP.B2 CNS Mice Yes (LY6A) Deverman et al., 2016 AAV-PHP.B3 CNS Mice ... AAV-PHP.eC CNS: neurons and astrocytes Mice No (LY6C1) Shay et al., 2023 AAV-PHP.V1* CNS...2020 AAV-PHP.N CNS: neurons Mice Yes (LY6A) Kumar et al., 2020 AAV-PHP.B4 CNS Mice...the more efficient AAV-PHP.eB, developed and deposited by the same lab. AAV-PHP.eB is the most commonly ... lower viral titers than AAV-PHP.B (e.g., 1 x 1011 vg/mouse), AAV-PHP.eB can transduce a greater number...AAV-PHP.eB (Fig. 3d and 4d, Jang et al., 2023). When combined with a specific promoter, AAV-PHP.AX shows...

... AAV-PHPeB Optogenetics Jonathan Ting New viral prep pAAV_BiSSTe10_dTomato_nlsdTomato AAV-PHPeB Control...AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control... AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_BiVIPe4_dTomato_nlsdTomato AAV-PHPeB Control...dTomato_nlsdTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_BiLAMP5e3_ChR2_mCherry AAV-PHPeB Optogenetics...dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_BiPVe4_ChR2_mCherry AAV-PHPeB Optogenetics...dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_BiPVe3_ChR2_mCherry AAV-PHPeB Optogenetics...dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_BiSSTe4_ChR2_mCherry AAV-PHPeB Optogenetics...

...packaged in AAV9-X1.1!  We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab at the Allen...Engel New serotype pAAV-hSyn-Cre-P2A-dTomato AAV PHP.eB Recombinases Rylan Larsen New serotype AiP13778...pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) AAV PHP.eB Recombinases Jonathan Ting New viral prep AiP13779...3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) AAV PHP.eB Recombinases Jonathan Ting New viral prep AiP13781...pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) AAV PHP.eB Recombinases Jonathan Ting New viral prep pAAV-CAG-GFP...Edward Boyden New serotype pAAV-hSyn-EGFP AAV PHP.eB Controls Bryan Roth New serotype AiP13044 - pAAV-AiE0779m...3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) AAV PHP.eB Controls Jonathan Ting New viral prep AiP13038...

...brain, would you recommend using the PHP.S capsid over the PHP.eB (the capsid we have been using)?Would...transduction in microglia? I have been using the AAV.PHP.eb for neuron, oligodendrocyte, and astrocyte specificity...Assisted Spectral Tracing (VAST) experiments using PHP.eB in the brain give some insight into codelivery ...blood brain barrier we would currently recommend PHP.V1 or AAV-BR1. We recommend you keep an eye out for...area.   Q8: On dense labeling in the brain with PHP.eB: A recent CLOVER collaboration injected 1e+13 vg...phenotypes (that’s almost 100ul/animal for the PHP.eBs that we have in house!), which seems to me a very... repository, this application is an outlier for PHP.eB (1x1011 - 1x1012 vg/mouse is more standard) and...

...pAAV.CMV.PI.EGFP.WPRE.bGH  AAV PHP.eB Controls Wilson New viral vector pENN.AAV.EF1a.eGFP.WPRE.rBG AAV PHP.eB Controls Wilson...pAAV-mDlx-NLS-mRuby2  AAV PHP.eB Controls Gradinaru New serotype pAAV-CaMKIIa-mCherry  AAV PHP.eB Controls Deisseroth...well as seven vectors newly available in the AAV PHP.eB serotype. We've also added two viral vectors that...New serotype pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE AAV PHP.eB Biosensor Kim New serotype AAV-mDlx-jGCaMP8s-... Wilson New serotype pAAV-CaMKIIa-EGFP AAV PHP.eB Controls Roth New serotype pAAV-hDlx-Flex-dTomato-Fishell...Deisseroth New serotype pAAV-Ef1a-mCherry  AAV PHP.eB Controls Deisseroth New serotype Looking for ...

...experiments delivered ChRgers by intravenous injection of PHP.eB, an AAV capsid capable of broad transduction of...need for intracranial injections. When packaged in PHP.eB, ChRgers and ChR2(H134R) both reach the brain, ...make it better suited for systemic delivery with PHP.eB compared to the existing ChR2(H134R).  In the second...higher-photocurrent properties when paired with PHP.eB’s systemic delivery enables minimally-invasive gene...resources on the Addgene blog Read more about the PHP.eB AAV capsid Read more about optogenetics Learn about...

...: pAAV-AiE0452h_3xC2-minBG-jRGECO1a-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science... pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science...: pAAV-AiE0452h_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science...

...and retinal cells (Issa et al., 2023). AAV-PHP.eB AAV-PHP.eB is a systemic capsid derived from AAV9 and...AAV2, AAV2-retro (AAVrg), AAV5, AAV8, AAV9, AAV-PHP.eB, AAV11 Heart AAV1, AAV8, AAV9 Kidney AAV8 ...

...Patriarchi New viral service pGP-AAV-syn-jGCaMP8m-WPRE PHP.eB Biosensors GENIE Project New serotype pAAV-hSyn-GRAB_OT1.0...serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls Feng New viral service, multiple serotypes...

...using AAV1-X1 (Chen et al., 2023); then, when AAV-PHP.eB was injected three weeks later, that LY6A transgene...transgene rescued the enhanced brain potency of AAV-PHP.eB.    Figure 4: AAV9-X1 and AAV9-X1.1 efficiently...

...CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=39322032   No terminator is 100% efficient...CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=17554393   Use of mammalian poly(A) ...

...transfer to the rat CNS: AAV PHP.EB vector dose–response and comparison to AAV PHP.B. Gene Therapy, 25(5), 392...

... al., 2020) derived from bacterial phytochrome (BphPs) photoreceptors have higher apparent cellular brightness...Brightness in HeLa cells vs. iRFP713, % d Ref BphP-based FPs miRFP670 https://www.addgene.org/79987...

...resources on the Addgene blog: Discover how the PHP.eB capsid was engineered Learn about the parts of ...

...left) and transduction patterns (right).  Both AAV-PHP.eB and the AAV.CAP-B capsids are derived from AAV9...

...hM3D is now also offered as AAV1 (along with AAV PHP.eB, AAV Retrograde, AAV2, AAV5, AAV8, and AAV9). Once...

... therapy trials (http://www.abedia.com/wiley/vectors.php). These clinical trials have been directed at...