CRISPR-based Genome Editing Systems For Editing in E. coli
Type
Blog Post
...plasmids with different induction systems, such as PT7, Prha, Ptet, and Para. The pDonor plasmid pRE57-Ter with..., we 1) replaced the promoter of gRNA-pMB1 with PrhaB, 2) changed the replicon of pCas into a non-temperature-sensitive...