...amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple, and gfasPurple) (Figure 2). Figure 2: Chromoprotein...expressed and gives the cell a magenta (amilCP) or purple (tsPurple) color. Though the toxin is integrated in ...). Eight years later, scientists isolated pink, purple, and blue chromoproteins isolated from reef-building... Figure 3: pTOX plasmids expressing amilCP and tsPurple genes. Image: Lazarus et al., 2019. One such... lab is the Waldor lab who used the amilCP and tsPurple chromoproteins to create negative selection markers... and Tse2 toxins, respectively, and encode the tsPurple chromoprotein.  Find the allelic exchange vectors...

...PLD). Now they present a set of variants called SuperPLDs with 10, 30, 50, and 100 times the activity of...other natural or synthetic head groups. The new SuperPLDs work with high efficiency in mammalian cells and...opening up a wide realm of possibilities. The CRY2-SuperPLD module is optogenetically recruited by different...endosome membranes) (Figure 4). The authors used SuperPLD to manipulate phosphatidic acid signaling (such...fluorescent labeling via click chemistry.    Figure 4: SuperPLD hydrolyzes membrane PC to PA. A) OptoPLD is recruited...293T cells expressing plasma membrane-targeted SuperPLDx30 (magenta) and PA probe GFP-PASS (green). After... indicated by the PA probe colocalizing with SuperPLDx30. Scale bar 10 µm. Images adapted from Tei et ...

...CTD, yellow; REC lobe, light blue; bridge helix, purple.   Apo Cas9 changes conformation frequently...CTD, yellow; REC lobe, light blue; bridge helix, purple; sgRNA, dark blue.   The REC lobe binds tightly...CTD, yellow; REC lobe, light blue; bridge helix, purple; sgRNA, dark blue.   DNA sequence recognition...CTD, yellow; REC lobe, light blue; bridge helix, purple; sgRNA, dark blue; target DNA strand, light gray...

... Gram positive bacteria will be stained blue or purple, and gram negative bacteria will be stained red...image below. An example of Gram positive (purple) and Gram negative (red/pink) staining. Image credit...within the peptidoglycan layer, staining the cell purple. When the alcohol is added, it dehydrates the peptidoglycan...staining procedure, gram positive bacteria are stained purple or blue, and gram negative bacteria are stained...

... Aavery in this Zoom video background. Want the purple bacteria video background with mascots Blugene ...and more!) on Addgene’s Help Center. Download the purple bacteria background.       Are none of those ...Addgene’s Science Careers blog topic. Download the purple lab-themed background.     We hope these science...

...right column of the plasmid page. Check out the purple arrow in the screenshot below to see what I mean...the dropdown to increase the length of the list (purple oval in the screenshot below). You can also use... of these "Associated Plasmids" is shown in the purple box below, where the bulleted list of plasmids ...

...made up of a fragment of the RPL13a promoter fused to a region of the RPL41 gene, to drive the selection...

...nuclease that targets and cuts genomic DNA. The interplay between Cas9 (which causes the breaks) and host...extremely effective as a genome editing reagent. This interplay falls into two broad categories and thus, causes...

...also find Dr. Page and Hello PhD on Twitter: @ThePurplePage and @HelloPhD "Role" call. #BLACKandSTEM what...do you do? — Stephani Page, PhD 👩🏾‍🔬💜 (@ThePurplePage) February 13, 2014 2. "The Scientist's Top...

...in blue; secondary antibodies are represented in purple; signaling molecules are represented in light green...occasionally ask themselves and others that most perplexing of questions: “who moved my goat anti-rabbit?...

...containing a polyhistidine tagged protein of interest (purple) and several contaminating proteins (blue, green...Ni2+-containing resin. The protein of interest (purple) binds to the Ni2+ and is immobilized along with...

...Expansion AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX Knowledgebase: A Data Resource for Multiplex ...

...Access, Seeding Labs partners with donors to supply surplus lab equipment to carefully-screened research and...

...attention. When I spoke with Daniel about the interplay of these two ideas he said “science is a form ...

...belongs to a different chemical family than the red/purple plant pigments found in all other plants, anthocyanins...

...the constant regions from an alternative isotype (purple, mouse). Depending on the cloning process, all ...

...Team! Why MTAs Matter Though they may seem like boilerplate drudgery to some scientists, MTAs are important—they...

...red-b-D-galactopyranoside on the assay paper turning it purple, allowing for easy visual detection of the presence...

...wall. Once in the recipient cell, the CRISPRi DNA (purple) flanked by left and right Tn7 end sequences (green...

...2023; data are smoothed using locally estimated scatterplot smoothing. (C) Number of requests per year for...