...induces degradation. In plants, auxin binds to TIR1 of the SCF-TIR1 ubiquitin ligase complex, promoting its ... mAID and expressing the essential plant protein TIR1 in non-plant cells, which interacts with the cells..., the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor, sufficiently suppresses basal degradation...

...system relies on a transport inhibitor response 1 (TIR1) expressed under a desired promoter with a specific...) sequence attached to your protein of interest. TIR1 interacts with endogenous Skp1 and Cul3 proteins...ubiquitin ligase complex. In the presence of auxin, this TIR1 complex binds to the degron sequence ultimately ...

...must be AID-tagged with the 25 kDa tag. Next, the TIR1 F-box protein must be expressed (inducibly, ectopically...ectopically, stably… it’s your call). The TIR1 F-box protein will associate with the endogenously expressed...of auxin hormone indole-3-acetic acid (IAA), the TIR1 F-box associated E3 ligase complex will ubiquitinate...

...expressed in a cell that also expressed auxin-sensitive TIR1, any protein fused with their AID tag could be targeted...they used CRISPR to create OsTIR1-expressing cell lines by inserting OsTIR1 into the "safe harbor" AAVS1...AAVS1 locus (a tet-inducible OsTIR1 plasmid is also available). Second, they co-transfected short-arm donor...