CRISPR 101: Epigenetics and Editing the Epigenome
Type
Blog Post
...Tet-dependent (TetO-dCas9-D3A) constructs. For lentiviral expression, Fuw-dCas9-Dnmt3a and Fuw-dCas9-Dnmt3a-P2A-tagBFP...and activate transcription. A lentiviral vector with the same modifier, Fuw-dCas9-Tet1CD, is available from...Core, as well as pLV-dCas9-p300-P2A-PuroR for lentiviral expression.
Figure 1: dCas9-p300 adds ...hUbC-dCas9-MQ1(Q147L)-EGFP is also available for lentiviral transduction.
Lysine-specific Demethylase 1 (... targeted enhancers. Although the lab used this vector in chick embryos, it also functions in mammalian...and this fusion allows for packaging into viral vectors and targeted delivery into hard-to-manipulate cell...targeting to a wider set of genomic targets. These two vectors are part of the larger pMVP/pMAGIC cloning system...