...reason, the top requested plasmid of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab...and VSV-G. This plasmid has been used in studies of the secretome, ubiquitin ligase, cellular senescence...example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors...lab! Lentivirus envelope vector pMD2.G This plasmid has been requested nearly 6,000 times (over 800 orders...orders in 2017) and expresses the VSV-G envelope protein under the control of the powerful mammalian CMV...determine the types of cells that it can infect. The VSV-G envelope is widely used and requested because it... types of cells. This means researchers who use VSV-G can deliver their genes of interest to a variety...

...Weinberg pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope protein...: Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope...export control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster...information on shipping plasmids to your country. Curious about how rabies plasmids are used in the lab? ...If you're an international researcher who uses plasmids containing genes from rabies and vesicular stomatitis...may have noticed it’s been easier to order these plasmids. This is because in 2024 certain export control...controls prescribed by the Australia Group, for these plasmids for the majority of the countries we ship to, ...

...protein for pseudotyping is VSV-G, or vesicular stomatitis virus G protein. VSV-G is a trimeric protein that...three plasmids: (1) The transfer plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These...one of the reasons VSV-G is used to transduce stem cells and neurons. However, VSV-G’s broad tropism can... on the envelope plasmid. Image from Crawford et al., 2020.   Broad tropism: VSV-G pseudotyping A commonly...cytotoxicity can vary. For example, the popular VSV-G envelope protein causes cells that it infects to...-based killing by anti-alphaGal antibodies. The VSV-G envelope protein is inactivated by serum regardless...surface to endocytose into the cell. The reason VSV-G is so commonly used is that it is good at infecting...

...available plasmid in the top 15. Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited...expressing plasmid, such as our second most requested plasmid of all time, pMD2.G. Find psPAX2. pMD2.G - Also...from the Didier Trono lab is pMD2.G, a VSV-G envelope expressing plasmid. Envelope proteins determine which...of applications. This plasmid was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (... published in 2003, this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation...envelope expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from David Sabatini... top requested plasmid from 2016. You may notice the “V2.0” at the end of this plasmid which indicates...

...γ-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G (Vesicular Stomatitis...glossary Plasmid Type Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein...in trans by specific plasmids. This method, therefore, reduces the number of plasmids that are required ...encoded by the transfer plasmid, but instead are provided in trans by other plasmids or the packaging cell...do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to...pseudotyped to alter infectivity): The use of the VSV-G envelope provides the widest tropism or range of...components are needed: γ-Retroviral transfer plasmid encoding a transgene, sgRNA, or shRNA of interest...

...pCMV-VSV-G Envelope Envelope protein; use with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...-expressing envelope plasmid Trono 1733 pCI-VSVG Envelope VSV-G recommended for the FELIX (FIV-based) ...Nienhuis and Salmon 22501 pHEF-VSVG Envelope VSV-G-expressing envelope plasmid Kasparov Popular γ-Retroviral...γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want a retroviral plasmid with your gene ...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...

...transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G N...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope plasmid Nienhuis... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Additional Resources... Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...and 3rd generation transfer plasmids Weinberg 1733 pCI-VSVG N/A Envelope VSV-G recommended for the FELIX...titer Kasparov 22501 pHEF-VSVG N/A Envelope VSV-G-expressing envelope plasmid Kasparov 22500 pNHP 2nd Packaging...

...envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles with the VSV-G envelope...consisted of three plasmids: the packaging, envelope, and transfer plasmids. The packaging plasmid carried a mutated...Hoyah G, Winemiller A, Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas...Many CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: ...different viral envelope protein (Env), usually from VSV-G due to wide infectivity, is often used as a substitute...itself due to a few missing proteins. The envelope plasmid contained a viral envelope to dictate the tropism...the vector could infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 ...

...Lentiviral vectors are pseudotyped in most cases with VSV-G that generally mediates broad cell tropism and has... share ways how scientists have used Addgene’s plasmid repository and viral service for their behavioral...behavioral studies. References Stoica L, Ahmed SS, Gao G, Sena-Esteves M. Gene transfer to the CNS using recombinant...

...these plasmids from Addgene. This publication then resulted in the development of a new plasmid, pCMV14...continue having access to plasmids during the COVID-19 pandemic. Over 7,000 plasmids for COVID-19 research...work to share plasmids to all scientists Resources on Addgene.org Find COVID-19 plasmids at Addgene Browse...the lentiviral production and packaging vectors VSV-G and psPAX2, which were used in a publication by ...Biological repositories that distribute mouse strains, plasmids, cell lines, and much more have been critical ...science is truly a global endeavor. Case study 2: Plasmids Another example of reagents that have broad applications...requested from Addgene. Figure 2: Total COVID-19 plasmids requested from Addgene from March 23, 2020 to ...

...with most lentiviral vectors being packaged using VSV-G. In contrast, AAV can be packaged in a variety of... The most obvious workaround is to use a multi-plasmid system, where you have one AAV vector dedicated...E., Kuhn, M., Farouni, R., Lagler, S., Kalamakis, G., Lischetti, U., Jabaudon, D., & Platt, R. J. (2023...Gessler, D. J., Zhan, W., Gallagher, T. L., & Gao, G. (2024). Adeno-associated virus as a delivery vector...

...gamma-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G, may be used ...separate envelope and packaging plasmids as well as transfer plasmids. This concept ensures safe handling...Learn more about CRISPR plasmids for transcriptional inhibition. Retroviral Plasmid Kit Retroviral Barcoding...expressing new shRNA sequences pLKO (Plasmid #10878) This plasmid has been used by The RNAi Consortium... genes and transgene expression across several plasmids. The most commonly used viral vector systems are...popular packaging and gamma-retroviral envelope plasmids here. Lentiviral vectors are very versatile tools...guide. Popular packaging and lentiviral envelope plasmids are listed here. Please note that both lentiviruses...

...usually encodes VSV-G, a packaging plasmid containing structural genes and a transfer plasmid carrying the... multiple plasmids. Second generation packaging systems use 3 plasmids; an envelope plasmid that usually...Rev gene from the packaging plasmid and incorporating it into a fourth plasmid. In addition, the promoter...and packaging plasmids in order to obtain the best titers. Requiring an additional plasmid for transfection...By dividing the viral elements across multiple plasmids, the risk of recombination and consequently, the...extra level of safety, they also require a fourth plasmid. The requirement for additional DNA can impact ...thereby affect the titer of the virus. Additional plasmids will also affect experiment optimization. Before...

...found that Nanoblades pseudotyped with a mix of VSV-G and the baboon endogenous retrovirus Rless glycoprotein...infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and viral transduction...types they tested. Interested in using Nanoblades? Plasmids are available from Addgene. Genome editing primary...to DNA transfection of gRNA and Cas9 expression plasmids. These results are in line with previous research...out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral production ...

...four different plasmids into the producer cells (two packaging plasmids, an envelope plasmid, and the lentiviral...envelope plasmid you use as the choice of envelope dictates the tropism of the virus.  VSV-G is very common...generation systems consist of an envelope plasmid and a packaging plasmid encoding HIV proteins gag, pol, rev...generation systems require an envelope plasmid, and two packaging plasmids, one encoding gag and pol and a second...transfer plasmids replication competent? What is SIN? A6: Most (if not all) Addgene transfer plasmids are ... 30% of the transfer plasmid. Many transfer vectors are SIN. Packaging plasmid(s) contain the minimal...Addgene's most popular lentiviral plasmids Overview of Lentiviral Packaging Plasmids Lentiviral Protocols and ...

...pseudotyping. Lentiviruses pseudotyped with the VSV-G envelope protein use the LDL receptor to infect ...after transfecting with the transfer and packaging plasmids. A benefit of the multiple harvest strategy is... B., Metharom P., Jullie H., Ellem K.A., Cleghorn G., West M.J., Wei MQ. “The significance of controlled...

...env gene (packaging plasmid), and 3) the vesicular stomatitis virus G protein (VSV-G) that improves the...virus (MoMLV) and consisted of two plasmids: the packaging plasmid containing all the structural genes...crossover event between the vector plasmid and the packaging plasmid caused the Psi sequence to be placed...expression of the transgene   Transfer plasmids The transfer plasmid is the vector you clone your gene of...usually 293T cells, with your transfer plasmid and your packaging plasmids. For this, you can use the transfection...techniques are still used and efficiently transfer plasmid DNA efficiently into dividing immortalized cells... (Gag, Pol, Tat, Rev and Env) and the transfer plasmid encoding the insert of interest as well as sequences...

...pseudorabies virus type 1, vesicular stomatitis virus (VSV; Beier et al., 2011), adenovirus, and adeno-associated... the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering...involved in learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California... throughout the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal...than any other AAV serotype (Tervo et al., 2016; plasmid at Addgene). Its efficiency is comparable to conventional...Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors ...F., Hisey, E., Tanaka, M., Kearney, M., Chattree, G., Yang, C. F., Shah, N.M., and Mooney, R. (2017). ...

...lentiviral plasmids Addgene's most popular retroviral plasmids Addgene's collection of AAV plasmids Addgene...split across several plasmids (often three), for safety purposes. The primary plasmid with the delivered...often a gene) is called the transfer plasmid while the other plasmids with packaging or replication components...of adenovirus plasmids Resources on the Addgene blog Parts of the AAV packaging plasmid Overview of Lentiviral...expression of the vesicular somatitis virus G glycoprotein (VSVG). VSVG is a broad tropism envelope protein that...For safety and production reasons, multiple plasmids with many components are used to package lab grown...can be intimidating at first to look at so many plasmid maps and protocols! In reality, there are many ...

...Envelope Plasmid Interchangeable: usually encodes for VSV-G Interchangeable: usually encodes for VSV-G Safety...integration. Packaging plasmid(s) Envelope plasmid Addgene’s packaging and envelope plasmids are generalized ...packaging systems Packaging Plasmid All on one plasmid: Gag, Pol, Rev, Tat Two plasmids: one encoding Gag and... 3-4 plasmids are transfected into A293T cells: one transfer plasmid one or two packaging plasmid(s) one...(usually VSV‐G due to its wide infectivity) is encoded on a third, separate, envelope plasmid. All 2nd...lentiviral transfer plasmids are based on the HIV-1 virus. For safety reasons, transfer plasmids are all replication...and optimize is the transfer plasmid. 2nd generation lentiviral plasmids utilize the viral LTR promoter...