...favorite protein (YFP). You have two options:  Option #1: Affinity tag purification You tag YFP and use an affinity...binding YFP to the column, you wash several times to remove non-specific proteins, and then elute YFP.  Option...Opto-Nanobodies (OptoNBs) purification You skip adding a tag to YFP and instead use OptoNBs. You fill a column with...the lights, OptoNBs bind YFP and non-specific proteins flow through. To elute YFP, you turn on the blue ...protein. OptoNBs were also tagged with an infrared fluorescent protein tag which let the team detect its... be tagged, which limits these approaches to use with recombinant proteins. Purifying an untagged protein...used nickel-coated agarose beads coated with his-tagged OptoNBs. OptoNB coated beads were then imaged while...

...GFP (green) to RFP, CFP, or YFP fluorescence. In Brainbow 2.1, GFP is tagged with a nuclear localization...Brainbow. Brainbow 1.0 used the fluorophores RFP (red), YFP (yellow), and membrane-tethered CFP (cyan). Brainbow...localize to a different area of the cell than CFP and YFP. The most recent derivation of Brainbow, Brainbow...incorporate a truncated, non-fluorescent form of YFP as the default state of the transgene such that recombination...recombination is necessary to excise the non-fluorescent YFP and activate fluorescence of the downstream OFP, ...can be detected by immunostaining of the truncated YFP. In Thy1-Brainbow mice, some neurons expressed as...called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream of a ubiquitous promoter. Zebrabow is ...

...variants of Yellow Fluorescent Protein (YFP). The team identified a new YFP variant, mGold. mGold was found to... as bright to mVenus, the parent YFP, and is the most photostable YFP to date. As such, mGold is expected...screening, Single-cell Phenotypic Observation and Tagging with Light (SPOTlight) as a way to isolate individual...

...C-terminal His tag SYFP2 Yellow Mammalian Express a gene of interest fused to the C-terminus of SYFP2 Clover... mCerulean/Amber, mCerulean/SYFP2A, mTurquoise/mVenus and others. CFP-YFP is still one of the best and...significant overlap in their spectra. The pair CFP-YFP was the first to be used to study protein-protein...Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian optimized...Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene of interest...popular pair, CFP has five-fold less brightness than YFP. The stoichiometry of donor:acceptor is difficult...

...solution or something in between, you are using a tag with a serious drawback. The standard EGFP plasmid... of a POI. As an investigator, you want a fusion tag to be inert, to not produce artifacts in your experiments...Several early FRET biosensors were made with CFP and YFP, cyan and yellow variants of EGFP equally capable...capable of dimerizing as EGFP. As a result, CFP and YFP dimers can form in the absence of environmental changes...other GFP family members (superfolder, Emerald, CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can...oligomers such as DsRed or high affinity FPs such as TagRFP. Problem 3 can be solved, in most cases, by using...

...is the ability to follow multiple fluorescently tagged species simultaneously. To do so with fluorescent...nm. For example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm / em 527 nm) can be imaged together but...multicolor imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed for...generally the most detectable and so should be used to tag your least abundant proteins, with the blue and infrared...#49004) to the spectra of both mCherry and TagRFP.  Here, the TagRFP spectrum is shown in the darker colors...peak of the TagRFP excitation and the emission filter collects a larger fraction of the TagRFP emission ...with this set are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared...

...Twelvetrees Lab took advantage of the self-labeling tags SNAPf and CLIPf. These tags rely on synthetic dye...single- and double-tagged constructs (including "CLAP", combining an N-terminal CLIPf tag and a C-terminal...of Gα-GTP by BRET. The biosensor consists of a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused...dicots Self-labeling tags for single-molecule biophysics Fast and responsive voltage indicator ONE-GO biosensors...2024.02.11.579803).  CLIP, CLAP, SNAP! Self-labeling tags for single-molecule biophysics By Mike Lacy In order...C-terminal SNAPf tag; see Figure 2), the authors developed a cost-effective expression and labeling approach ... The team deposited an array of constructs for tagging the kinesins KIF5A and KIF5B as well as backbones...

... contains three fluorescent proteins: RFP (red), YFP (yellow) and M-CFP (membrane tethered cyan). Without...expressed. Cre can mediate one of two deletions to allow YFP or M-CFP expression; these deletions are defined ...construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem invertible...the promoter. The STOP cassette includes a mutant YFP that does not fluoresce, but can be detected via ... (AAV) available - AAV-EF1a-BbChT and AAV-EF1a-BbTagBY. These constructs contain two XFPs each due to ...

...against popular fluorescent proteins (GFP and YFP) and protein tags (c-MYC and HA). We have also created the...

...encoding alternative promoters and/or fluorescent tags are available at Addgene. Shemesh OA, et. al. ...ShadowY, a novel dark yellow fluorescent protein (YFP) with an excitation peak of 519 nm. These proteins...microscopy) assays. ShadowG is darker than other dark YFP mutants, it reduces the risk of spectral contamination...change that placesTagRFP-T and Dronpa in close proximity. By monitoring changes in TagRFP-T fluorescence...FLINC)-based biosensors measure fluctuations of TagRFP-T fluorescence intensity due to interactions with...membrane in living cells using a construct in which TagRFP-T and Dronpa flanked a PKA substrate fused to the...

...spectra. A comprehensive list of cell markers all tagged with mEmerald. Plasmids to monitor protein-protein... the standard oldies but goodies (e.g., EGFP and YFP) to the new and improved fruit colors (e.g., apple...available targets since the Davidson lab always tagged new proteins with mEmerald first, Baird says. From...cell membrane, this collection has fluorescently tagged versions of many proteins known to localize to ...Democrat in a story about his life’s work and the stage IV lung cancer that has now prompted him to deposit...

...specificity of their system, Tang et al. found that YFP and CFP can activate certain T-DDOG constructs similarly...GFP), is activated by GFP and derivatives GFP and YFP, but not red fluorescent proteins, as seen with T-DDOGs...optogenetics in specific cell populations even easier. Advantages and other possibilities It’s clear that Cre-DOG...GFP-labeled lines, and they each have their own advantages. T-DOGGs can be made drug-inducible using the...

...can be used to target any protein fused with GFP, YFP, mCerulean, or other related fluorophores. Find all...labels proximal proteins with biotin as an affinity tag. This ‘Caspex’ construct is guided by a pre-defined...biotinylation at genomic elements by restricted spatial tagging), to confirm the composition of proteins found ...antibodies found in camelids. They possess many advantages over conventional antibodies: they are small ...

...variants blue FP (BFP), cyan FP (CFP), and yellow FP (YFP) were derived. For a breakdown of GFP, it's variants...CFP) excites the fluorescence of another FP (e.g. YFP). FRET only occurs when the distance between the ...pHTomato (pH sensors), HyPer (H2O2 sensor), ArcLight (voltage sensor), and iGluSnFr (glutamate sensor). More ...can fold in <10min at 37°C, mCherry takes ~15min, TagRFP ~100min and DsRed ~10hours. Temperature: FPs maturation...is set as 1. Some proteins are very dim (e.g. TagRFP657, which has a brightness of 0.1) and this should...

...times brighter than the most commonly used GFPs and YFPs. Its excitation maxima is at 506 nm and its emission...respectively. mNeonGreen can be used as a fusion tag for a wide range of applications from traditional...from the tetrameric yellow fluorescent protein (LanYFP) from the marine invertebrate Branchiostoma lanceolatum...Institute developed mNeonGreen via 21 substitutions to LanYFP and the addition of the enhanced GFP (EGFP)-type...was then screened through successive rounds of mutagenesis for the brightest teal fluorescent proteins that...scientists from Florida State University, they used a mutagenesis approach and found that specific amino acid changes...

...T2A-TurboGFP-PEST), and small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The Foerstemann lab developed... scarless tag insertion.   In addition to the obvious advantages SapTrap offers for tagging a single genetic...between the tag and homology arms). Digestion of the donor plasmids with SapI allows the tag, selectable... inserting a tag at multiple target sites. SapTrap vectors can be easily modified to tag hundreds of genes...insertion tagging), developed by the Hornung Lab, allows users to easily create C-terminal tag fusions ... toolkit offers C- and N-terminal tagging vectors (eg GFP, Flag, YFP, Strep, TEV-V5) with either blasticidin...endogenous protein tagging, please visit our website CRISPR/Cas Plasmids - Protein Tagging. Beyond simply...

...development in real time.  In both cases, dL5** was tagged with mCerulean3 (mCer3) for visualization and expressed...small amounts of non-specific ROS when exposed to YFP and RFP excitation wavelengths, which suggests that...xOtH5bs4HiI Advantages of MG-2I-dL5** over other photosensitizers One of the main advantages of MG-2I-dL5...

...pRS416Gal TDP43 WT YFP TARDBP YFP GAL1 ALS Aaron Gitler 27448 pRS416 Gal G294A YFP TARDBP YFP GAL1 ALS Aaron...pRS416 Gal M337V YFP TARDBP YFP GAL1 ALS Aaron Gitler 27450 pRS416 Gal Q331K YFP TARDBP YFP GAL1 ALS Aaron...pRS303 Gal TDP-43 YFP TARDBP YFP GAL ALS Aaron Gitler 27470 pDONR TDP43 WT YFP TARDBP YFP ALS Aaron Gitler...Gitler 29592 426Gal-FUS-YFP FUS YFP GAL1 ALS Aaron Gitler 29593 416Gal-FUS-YFP FUS YFP GAL1 ALS Aaron Gitler...29594 416GPD-FUS-YFP FUS YFP GPD ALS Aaron Gitler 29595 416Gal-SV40NLS-FUS-YFP FUS YFP, SV40 NLS GAL1 ALS...426Gal-FUS-1-170aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29597 426Gal-FUS-1-270aa-YFP FUS YFP GAL1 ALS Aaron ...426Gal-FUS-1-373aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29599 426Gal-FUS-1-413aa-YFP FUS YFP GAL1 ALS Aaron ...

...terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG...

...C-terminal fusion of a tetracysteine tag. In addition, since these tags are so small, one can insert them... lure the ribosome into inserting a fluorophore-tagged amino acid at a stop codon (often amber i.e. UAG...termination at stop codons, expression levels of these tagged proteins are often very low. In addition, a major...which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have a FRET acceptor-binding...: you just don’t know it yet! The poly-histidine tag often used for protein purification is a reliable...for protein purification: the NTA binds to the His tag on your protein and the coupled Cy fluorophore acts...well as by extending the length of the histidine tag from 6 to 10 residues. While these Cy/NTA conjugates...