Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 69 results for: addgene/1000

Showing: 1 - 20 of 69 results

  1. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ... Commercial Plasmid Prep Kit Amount 1000 Lanes 1000 Lanes 1000 Lanes Cost $200-$500  <$10 $20-30 ...molecular weight ladders were recently deposited with Addgene. A team of undergraduate students led by Dr. Song...convenient, commercial ladders cost between $200-$500 for 1000 lanes worth of ladder. The Penn State ladders are...plasmid prep protocol, it cost less than $10 to make 1000 lanes worth of the 100 bp or the 1 kb ladder. Using...pPSU1 and the pPSU2 plasmids are available from Addgene. Learn How to Analyze a Restriction Digest References...Central PMCID: 5446395. Additional Resources on the Addgene Blog Are you a Frugal Scientist? Save Money with...the Evolution of Lab Techniques Resources on Addgene.org Use the Kit-Free Alkaline Lysis Plasmid Miniprep...

  2. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Communications Manager Addgene's book club Indeed, reading is ingrained into Addgene’s culture. We have a ...still going on today! Addgene Book Club (ABC) founder, Eric Perkins, is Addgene’s Director of Science Product... of Addgene’s culture. Have you read any of these books? Whether you have or not, let the Addgene community...Additional resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities...From Addgene Book Club to our on-site book swap/library, reading is part of the culture here at Addgene...larger epic of fantasy novels. Set in a world where 1000 years ago the hero lost and the Dark Lord rules ...Napoleonic Wars and against something even more sinister. 1000 pages long. Incredibly imaginative, funny, bittersweet...

  3. Lentiviral Prep Service

    Type
    Collection
    ...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently...genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone does not contain...unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root 73179...genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9...unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root Human... unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls. This backbone does not contain...

  4. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    ...All plasmids deposited at Addgene enhance scientific sharing worldwide but a select few are particularly...particularly special. Over 1000 of our 60,000+ deposited plasmids have been requested more than 100 times each...we know biological resource centers, including Addgene, positively affect scientific research. As Maria...Maria Castro of University of Michigan says, “Addgene has transformed the way in which we share plasmids...tool is so influential that it is requested from Addgene many times as the technology disseminates throughout...researchers who have at least one plasmid deposited at Addgene that has been distributed more than 100 times. ... on sharing because, as UNC’s Bryan Roth says, Addgene has “literally enabled sharing with 100's of investigators...

  5. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...As part of our mission, we’ve been working with Addgene to develop and apply our plexWell™ Library Preparation... for NGS-based sequencing and confirmation of Addgene’s large and growing collection of curated plasmids...are using this technique in our partnership with Addgene to sequence large numbers of plasmids. plexWell...barcodes. Rather than continuing to process 100s to 1000s of samples individually, plexWell™ reduces every...does not vary by DNA input amount. plexWell™ + Addgene = high quality NGS-assembled plasmids Because of...original sample. At seqWell, we have worked with Addgene to develop a highly optimized bioinformatics pipeline...circularized plasmids from plexWell™ NGS data. Once Addgene produces plates of plasmid DNA, they are shipped...

  6. Antibodies 101: ChIP

    Type
    Blog Post
    ... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...pieces of roughly equal size, typically between 200-1000 bp. This is to ensure that your downstream analysis...  References and Resources More resources on Addgene’s blog Antibodies 101: Immunoprecipitation  Antibodies...

  7. The time and cost required to make a plasmid

    Type
    Blog Post
    ...Plasmids 101 eBook Resources on Addgene.org Join the community of Addgene depositors here Check out our...intervening five years, inflation has occurred. Here at Addgene, the consumables cost per stab has increased 50%...plasmids. Use repositories Repositories such as Addgene help share materials quickly and cheaply. We save...last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means we virtually always...community engagement with and use of repositories like Addgene provides large-scale savings. How large? Using ...average of 5 hours of hands-on time per plasmid, Addgene, since its founding in 2004, has saved the scientific...and very cool 8,768,560 hours – or almost exactly 1000 calendar years!   *Dammit, man, I’m a scientist...

  8. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...proximity ligation." Nature methods 3.12 (2006): 995-1000. PubMed PMID: 17072308.  4. Dieck, Tom, et al. "...PMCID: PMC4121734. Additional Resources on the Addgene Blog Fluorescent microscopy techniques - Which...protein-protein interactions Deposit plasmids with Addgene! ... tagging strategies Additional Resources on Addgene.org Fluorescent protein plasmids Use FRET to study...

  9. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral Guide...particles. Studies have found that values can be 10-1000 fold higher than for functional assays depending... PMCID: PMC514534. Additional Resources on the Addgene Blog Read Our Lentivirus FAQ Learn How Lentivirus...

  10. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...More examples of these biosensors can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be...coefficient and quantum yield of the protein, divided by 1000. In many cases the brightness is compared to that...More Helpful Websites & Resources: FP guide at Addgene Interactive Visualization of Fluorescent Protein...

  11. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...Since Addgene began producing AAV vector preparations, we have used quantitative PCR to evaluate the ... qPCR can be challenging to get right. Here at Addgene, we use a linearized plasmid standard. However,...virus before adding your sample to a mastermix. At Addgene, we typically load 3 dilutions onto our ddPCR plate...used to calculate the viral titer: GC/mL = {[(R*C)(1000/V)]*D} R = Reaction volume C = Copies/uL V = Volume...PMCID: PMC5167268. Additional resources on the Addgene blog Read more about polymerase chain reaction... vector related protocols Learn more about the Addgene viral service ...of our viral vectors blog posts Resources on Addgene.org Learn our protocol for AAV titration by qPCR...

  12. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ... (response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target...writing of this article. Additional Resources on the Addgene Blog Browse All Plasmids 101 Posts Learn about...Reporter Gene like Luciferase and GFP Resources on Addgene.org Find Plasmids for Your Research     ...

  13. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...proteins are quite large — comprising approximately 1000–1400 amino acids — which poses a challenge for efficient...top-performing constructs are now available through Addgene (pKb-TnpB1 and pKb-TnpB2). How TnpB Works: The...025-02023-8    Additional Resources on the Addgene Blog CRISPR 101: Engineering the Plant Genome ... in Popular Culture Additional Resources on addgene.org Genome Engineering Guide Plant Plasmids and ...

  14. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...includes SpCas9, and shortern orthologs approximately 1000 amino acids in size. From the pool of shorter orthologs...j.cell.2020.03.023 Additional Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded...Cpf1 Read our Introduction to AAV Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids...

  15. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...of template should have homology arms between 500-1000 bps. HDR efficiency with circular plasmid templates... (2017). PubMed.  Additional Resources on the Addgene Blog Check out our CRISPR Featured Topic Page...CRISPR Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs...

  16. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...available through Addgene. This means you could perform a screen with one library from Addgene, and then use...design 5 guide RNAs (gRNAs) targeting each of the 1000 genes you’d like to investigate in your next CRISPR...PMCID: PMC5292764. Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design ...melanogaster, and T. gondii are available through www.addgene.org/crispr/libraries/. Additional libraries are...gRNA Tips for a 1st CRISPR User Resources on Addgene.org Learn More About CRISPR Find CRISPR Screening...

  17. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...4(9) (2015):987-1000. PubMed PMID: 25822415. Find plasmids from this paper at Addgene. 12. Ronda, Carlotta...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Browse Our CRISPR Topic...add multiple gRNAs on one plasmid. Sound good? Addgene has many tools to help you multiplex - we’ll use...Multiplexing gRNAs: the basics One common question Addgene Senior Scientists receive is: can I express more...Two Golden Gate options that are available from Addgene follow these same assembly principles, but they...cassette.    Find gRNA multiplexing vectors at Addgene! Multiplexing in plants Qi-Jun Chen Lab Golden ... PMC4270925. Find plasmids from this paper at Addgene. 2. Kabadi, Ami M., et al. “Multiplex CRISPR/Cas9...

  18. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...Low 572/ 610 FP595 Tetramer Green Dark/ Red 70 - 1000 Medium 590/ 600   Choosing a photoactivatable...PubMed PMID:18647110. Additional Resources on the Addgene Blog Read More about Fluorescent Protein Imaging...Catch Up on Your GFP Background Resources at Addgene.org Find Photoactivatable FPs in Empty Backbones...

  19. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...plasmids used for these methods can be found on Addgene’s stem cell page. On this page, you can also find...fibroblasts to myoblasts. Cell, 1987. 51(6): p. 987-1000. PubMed PMID: 3690668. 40. Ferber, S., et al., Pancreatic...PubMed PMID: 18940730. Additional Resources on the Addgene Blog Stem Cell Models for Disease & Open Science... can be used for gene delivery Resources on Addgene.org Visit our Stem Cell Pages Find Viral Vectors...

  20. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...also like... Viral Vector Guides Virus Blog Posts Addgene Protocols Viral Service Introduction This protocol...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette CO 2 incubator Pipet controller...concentration that was used for the initial selection). Addgene recommends that the use of selective medium throughout...cell lines. When this protocol was performed at Addgene on the A549 cells described in our sample data ...96-well plate, seed one of the corner wells with ~1000 cells. This will help you focus the microscope later... incubating the cells. Pro-Tip Use the well with 1000 cells to focus the microscope, then you can easily... 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved vectors...
Showing: 1 - 20 of 69 results