...and containers. Water baths can also be placed in walk-in refrigerators to achieve a temperature between...

...and purity click here . Top Protocol: Kit-free Alkaline Lysis Plasmid Miniprep Prepare the following solutions...

...Delta-lacX74 recA1 araDelta139 D(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG tonA::Ptrc ccdA DB3.1 Invitrogen...sr1-recA) mcrB mrr hsdS20 (rB- mB-) supE44 ara14 galK2 lacY1 proA2 rpsL20(StrR) xyl5 lambda- leu mtl1 DH5alpha...Tn10 (TetR) ∆(ara-leu) 7697 araD139 fhuA ∆lacX74 galK16 galE15 e14- Φ80dlacZ∆M15 recA1 relA1 endA1 nupG...mcrB mrr hsdS20 (rB–, mB–) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20 (StrR ) xyl-5 λ– leu mtl-1 Top10...Delta-lacX74 recA1 araD139 Delta(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG Antibiotics commonly used...

...Science Talk." 4. The chalk talk, simple in principle The first question I usually get about chalk talks...Developing your Chalk Talk These items should be part of the beginning of your chalk talk. These should ...what you expect. For the rest of the chalk talk, as with your job talk, avoid the temptation to provide too...This edition has substantial expansions on the chalk talks and Zoom interviews sections, along with new ...talks is, what is a Chalk Talk? It is a 60-90 min session with department members to discuss your research... whether there is chalk. Sometimes. It can also be a slide deck, markers, etc. Chalk or markers force ...alternatives for an approach, if asked. The key to a chalk talk is to effectively convey your message. Key Things...

...poster or talk at the conference, you can end by asking them to come to your poster or talk. After the...conference Take notes during talks. If you have questions during the talk, jot them down for the Q&A immediately...it down into the following: the elevator pitch, talks, the poster session, networking, and social media...information to prepare for the next meeting. Navigating talks Before the conference Is the conference you are ... At large conferences, chances are that several talks will be going on at once in different rooms. Before... a cool new method that you learned about at the talk, bring it to your next lab meeting. Don’t be afraid...reach out to them, pointing out that you heard their talk at the meeting and why you think a collaboration...

...address was the first of 19 talks given over the course of the day. His talk, which focused on the history...will not be attending the entire meeting, Monday's talks alone were worth the trip. Dr. Dana Carroll's excellent...Advances in Genome Engineering", brought us 8 short talks on every conceivable way of examining, modifying...malaria-causing Plasmodium falciparum. Throughout these talks, I oscillated between amazement at how much has ...implementation of this system. Monday evening's talks switched gears (or should I say circuits?) to more...fooled myself into thinking I was an engineer. The talks we heard Monday night represented engineering first...circuitry to the downstream output. Tuesday morning's talks were also about circuitry and synthetic biology,...

...Φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG fhuA::IS2 DB3.1 Streptomycin...glnV44 Δ(sr1-recA) mcrB mrr hsdS20(rB-, mB-) ara14 galK2 lacY1 proA2 rpsL20(Smr) xyl5 Δleu mtl1 DH10B Streptomycin...screening, leucine auxotroph. F- endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu...hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ- JM109   General...plasmid after preparation. rpsL thr leu thi lacY galK galT ara tonA tsx dam dcm glnV44 Δ(lac-proAB) e14...strain. F- Δ(ara-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15 λ- e14- mcrA0 relA1 rpsL150(strR) spoT1 ...Tn10 (TetR) ∆(ara-leu) 7697 araD139 fhuA ∆lacX74 galK16 galE15 e14-  Φ80dlacZ∆M15 recA1 relA1 endA1 nupG...

...option if there is no cross-talk between FRET pairs. Unfortunately cross-talk between fluorophores does ...biologist you would say. The student was of course talking about the now well-known FRET, aka Fluorescence...fluorophore pair, donor:acceptor stoichiometry, and cross-talk between the two fluorophore colours. Experts in ...keep in mind when analysing FRET results. The cross-talk between the two fluorophores is linked to their ...used to excite the donor. In that case the cross-talk is high and your background signal may be higher...too far from each other, you don’t have any cross-talk but you also don’t have any resonance transfer. ...find a balance between FRET efficiency and cross-talk. Methods to Measure FRET for Cell Biology Studies...

...fluorescent proteins at Addgene. 7. Designing Your Chalk Talk for the Academic Job Interview by Erik Snapp ...blogger Erik Snapp breaks down how to prepare a chalk talk for an academic job interview including defining...research vision, organizing your vision into a chalk talk, and tips for practicing.  6. The Fluorescent...

...   If you don’t already make it a habit to talk – really talk – about the research you are doing in the...'s important for scientists to get out there and talk about their work. To tell others why that work is...academia, and that means it’s important to know how to talk about science to everyone – not just to others working...   Consider starting up your own twitter feed or talking about and sharing some science on the one you already...Carolina Research Triangle area, scientists give talks and science cafés at the North Carolina Museum of...

...potential solutions. Prior to this event, I gave my own talk on reproducibility at Addgene and here I summarize...summarize what I learned both in preparation for my talk and at the minisymposium. You can find a variety of...reproducibility As Jeffrey Flier points out in his talk (1:24 - 29:31), there are a variety of ways of defining...pulled from a freezer. As you’ll see in Steve Almo’s talk about work at the Institute for Protein Innovation...more reliable reagents for future research. I’ve talked to many researchers who have complaints about the...want to read. As Susanna Bachle points out in her talk (29:31 - 49:45), there are many ways to publicise...better experimental design practices including talking to statisticians when necessary and encouraging...

...general intro until you know if the person you are talking to is really in your direct area.  Review the conference...problems or questions you are pondering and be ready to talk about them. People love to help especially when ...someone since you are both interested in the same talk. Read more on this advice to “never leave an empty...with, “Is this seat taken? Can I join you?” Next talk about the food and then move on to science. More...on conference meal etiquette are here. Don’t only talk about science and work – casual socializing is how...that are memorable. It isn’t always appropriate to walk up to someone much more senior than you, but you...

...the middle of your talk. Technical difficulties turn your slide deck into a chalk talk. Your translator ...keep going no matter what, to fill any silence, to talk. That moment taught me an incredibly valuable lesson...When my mind went blank, my options were to stop talking until I could present something coherent or to ...You get nervous and freeze, or forget a point, or talk extremely quickly. You realize, much too late, that...

...RNA. One talk that really brought this home was presented by Todd Lowe from UCSC. Dr. Lowe talked about ... those of the CRISPR variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and... at the bench. It was a fantastic opportunity to talk to so many scientists who were potential Addgene... there were no English speakers around. Opening talks – Genomics discoveries and moving beyond DNA Eric...started off with the first keynote presentation. He talked about the impact of the Human Genome project on...

...afford them). I am talking about making sure the audience is interested, educated and talking as a result of...chose good panelists, they are going to be good at talking, so they will easily take 5 minutes to introduce...if discussion is lively). Next time you are at a talk and the speaker goes over 1 hour, watch what happens...'s job to politely but firmly get them to stop talking and redirect the conversation. The person you cut...

...to give a job talk for a remote interview. Fielding questions can be hard during this talk since you’ll... deliverable. Find 10 steps to a perfect science talk Be prepared for potential technical difficulties... your interviewers took time out of their day to talk with you, so be engaged with their conversation.... questions after completing each section of your talk. Some video conferencing software allows participants...

...into volunteering, I was talking to a little girl about fossils. After we talked for about ten minutes, ... new friends. Without the encouragement and pep talks from these contacts, I would have quit my “nontraditional...opportunities with an open mind and open ears. Everyone I talked to provided new data. Every new experience taught...graduate office staff was amazingly supportive. I talked to them about my interests and my ideas all the...

...every person you talked to during the day was also a scientist. But now you also need to talk to Tony from... bay for 8-12 hours a day become your family. I talked to a graduate student at a networking event recently...Going home at 5 o’clock. You’re going to have to talk and interact with...non-scientists! Maybe you’ve...interactions, but you could also embrace them. Talking about science to non-scientists can be a fun and...

... based on a bacterial haloalkane dehalogenase that binds a synthetic chloralkane (CA) ligand. The current...magic, but it’s real! In this post, we’re going to talk about the power and flexibility of self-labeling...fluorophore in your protein or to incorporate an alkene to be labeled through click chemistry. Although...  (TMP-tag) Few Easy HaloTag 295 Chloralkane (CA) Many Easy CLIP-tag 182 Benzylcytosine...

...means that you need to introduce yourself to and talk with more senior scientists. 3. Work intensely, ...often Surprisingly, many PFs are reluctant to give talks or posters about their research. They dread giving...answering questions. Organizing a thoughtful engaging talk takes significant effort. It requires evaluation...group and tack on a few new pieces of data, your talk will probably not inspire you or the audience. Ask...the current literature. Finally, if you give your talk or poster to enough different kinds of audiences...