Viral Vectors 101: Gamma-Retroviral Packaging Systems
Type
Blog Post
...ecotropic envelope, Phoenix-ECO, (for infection of mouse and rat cells), or an amphotropic envelope, Phoenix-AMPHO...polymerase.
Envelope gene (may be pseudotyped to alter infectivity): The use of the VSV-G envelope provides...γ-retrovirus using different envelopes to modify tropism. Briefly, different envelope plasmids can direct the...Purpose
Envelope
VSV-G
Vesicular stomatitis virus G glycoprotein; Broad tropism envelope protein;...packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G (Vesicular Stomatitis ...methods. The three components described above (envelope, packaging, and transfer) are supplied by three...nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses can transduce both dividing and ...