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We narrowed to 3 results for: amphotropic envelope

Showing: 1 - 3 of 3 results
  1. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post
    ...ecotropic envelope, Phoenix-ECO, (for infection of mouse and rat cells), or an amphotropic envelope, Phoenix-AMPHO...polymerase. Envelope gene (may be pseudotyped to alter infectivity): The use of the VSV-G envelope provides...γ-retrovirus using different envelopes to modify tropism. Briefly, different envelope plasmids can direct the...Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope protein;...packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G (Vesicular Stomatitis ...methods. The three components described above (envelope, packaging, and transfer) are supplied by three...nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses can transduce both dividing and ...
  2. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...used viral vector systems are made up of separate envelope and packaging plasmids as well as transfer plasmids...and find popular packaging and gamma-retroviral envelope plasmids here. Lentiviral vectors are very versatile...Lentiviral guide. Popular packaging and lentiviral envelope plasmids are listed here. Please note that both...packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G, may be used across both...genes via infection with a single ecotropic or amphotropic MuLE lentivirus provides significant genetic ...
  3. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...ecotropic envelope, Phoenix-ECO (for infection of mouse and rat cells), or an amphotropic envelope, Phoenix-AMPHO...changing the envelope gene, a process called pseudotyping. VSV-G is by far the most common envelope gene, as... and integrase components. Envelope plasmid env in trans The viral envelope gene; typically vesicular ...plasmid — contains packaging genes gag and pol Envelope plasmid — contains packaging gene env ; usually...stomatitis virus G protein) is the most common envelope gene, due to a wide range of infectivity for different... methods . The three plasmids described above (envelope, packaging, and transfer) are co-transfected into...to pseudotype gamma-retrovirus using different envelopes to modify tropism, as the env gene can easily ...
Showing: 1 - 3 of 3 results