...dSpyCas9-APEX2 fusion.  This Sontheimer lab used this technology, which they call C-BERST (dCas9–APEX2 biotinylation...tyrosine sulfation consensus sequence, fluorophore, or APEX2), allowing for quantitative analysis using different...catalytically inactive Cas9 (dSpyCas9) fused to APEX2 is expressed from a Tet/Shield1 inducible CMV promoter... cells are treated with biotin–phenol and H2O2, APEX2 labels electron-rich amino acid side chains (e.g...

...enzymes most frequently used for PL were APEX2 and BioID. APEX2 is fast, tagging proximal proteins within...yeast display-based directed evolution to create APEX2. They have now applied the same directed evolution...overcome both the speed and toxicity limitations of APEX2 and BioID. In the Ting Lab’s recent Nature Biotechnology...non-toxic biotin, but label nearly as quickly as APEX2. What’s more, since the enzymes were ”evolved” in...

...Lam et al. 2014). They named this mutant APEX2.  Find the APEX2 plasmids! TurboID and minturbo: Rapid and...locations in the cell.   Find BioID2 plasmids here APEX2: A more sensitive ascorbate peroxidase for proximity...and BioID2 can take hours to label proteins and APEX2, although it can rapidly label proteins, requires...Lam, Stephanie S., et al. "Directed evolution of APEX2 for electron microscopy and proximity labeling."...

..., TurboID and APEX2, are localized to different cellular compartments. They showed APEX2 can use an orthogonal...TransitID uses sequential labeling by TurboID and APEX2 at different cellular locations. C) Enrichment of...

...an improved peroxidase reporter, APEX2, through yeast display. APEX2 has been shown to exhibit superior...Brown et al., Genesis 2014 Jan;52(1):39-48. APEX2 for proteomic mapping and electron microscopy A ...greater sensitivity at lower expression levels. APEX2 is a monomeric, 27 kD genetic tag which can be fused...

...poor spatiotemporal resolution for in vivo work. APEX2, a faster peroxidase-based labeling approach, is...

...2017). In this technique, the receptor fused to APEX2 catalyzes the biotinylation of proteins near the...

...His T7 ALS Anni Vedeler 164622 3xflag-APEX2-LRRK2 LRRK2 Flag, APEX2 CMV Parkinson's Mark Cookson 165107 ...Cbh Episodic ataxia Walker Jackson 129705 Twinkle-APEX2-V5 TWNK CMV Spinocerebellar ataxia, infantile onset...pHAGE-eGFP-OPTN OPTN ALS Wade Harper 175760 pHAGE-FLAG-APEX2-OPTN OPTN Flag CMV ALS Wade Harper 175762 pHAGE-...